Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Genetic Authentication of Cynanchi Wilfordii Radix and Cynanchi Auriculati Radix by Using Conventional-PCR and Real-time PCR

Conventional-PCR 및 Real-time PCR을 이용한 백수오와 이엽우피소의 유전자 종감별 시험법 비교

  • Ryu, Hoe Jin (Medicinal Herb Inspection Team, Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Kim, Ae Kyung (Medicinal Herb Inspection Team, Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Kim, Sung Dan (Medicinal Herb Inspection Team, Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Jung, Sam Joo (Medicinal Herb Inspection Team, Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Jang, Jung Im (Medicinal Herb Inspection Team, Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Lee, Hee Jin (Medicinal Herb Inspection Team, Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Lee, Jung Mi (Medicinal Herb Inspection Team, Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Yu, In Sil (Medicinal Herb Inspection Team, Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Jung, Kweon (Medicinal Herb Inspection Team, Seoul Metropolitan Government Research Institute of Public Health and Environment)
  • 류회진 (서울특별시보건환경연구원 한약재검사팀) ;
  • 김애경 (서울특별시보건환경연구원 한약재검사팀) ;
  • 김성단 (서울특별시보건환경연구원 한약재검사팀) ;
  • 정삼주 (서울특별시보건환경연구원 한약재검사팀) ;
  • 장정임 (서울특별시보건환경연구원 한약재검사팀) ;
  • 이희진 (서울특별시보건환경연구원 한약재검사팀) ;
  • 이정미 (서울특별시보건환경연구원 한약재검사팀) ;
  • 유인실 (서울특별시보건환경연구원 한약재검사팀) ;
  • 정권 (서울특별시보건환경연구원 한약재검사팀)
  • Received : 2018.01.03
  • Accepted : 2018.02.23
  • Published : 2018.03.31
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Abstract

Recently, it has been a big issue to distinguish the dried roots of Cynanchum wilfordii and C. auriculatum in health functional food market. The original plant species of Cynanchi Wilfordii Radix belong to the Asclepiadaceae family is differentially described in the national pharmacopoeia of Korea, China and Japan. Owing to the morphological similarities of the dried roots of this plant to those of C. auriculatum, which is often misidentified in Korean herbal medicine marketplace, distinguishing these two species is exceedingly difficult. The purpose of this study was to compare the conventional-PCR with the real-time PCR for detection of C. wilfordii and C. auriculatum DNA. We also tried to realize a quantitative real-time PCR assay using species-specific matK primers, which allowed us to estimate the ratio of C. willfordii and C. auriculatum using varying ratios of mixed genomic DNA template from the two species. The differentiation of intentional and unintentional mixture in this study would be applied to food safety management and can be helpful for protection of consumer's right and cultivators.

Keywords

References

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