생약학회지 (Korean Journal of Pharmacognosy)

  • 제49권1호
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  • Pages.70-75
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  • 2018
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  • 0253-3073(pISSN)
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  • 2288-9299(eISSN)
한국생약학회 (The Korean Society of Pharmacognosy)
권호 표지

UPLC-MS/MS를 이용한 산천목 중 10종 성분의 함량 분석

Quantitative Analysis of the Ten Phytochmicals in Acer tegmentosum Maxim by UPLC-MS/MS

  • 황윤환 (한국한의학연구원 한의기술응용센터) ;
  • 이위 (한국한의학연구원 한의기술응용센터) ;
  • 양혜진 (한국한의학연구원 한의기술응용센터) ;
  • 마진열 (한국한의학연구원 한의기술응용센터)
  • Hwang, Youn-Hwan (KM Application Center, Korea Institute of Oriental Medicine) ;
  • Li, Wei (KM Application Center, Korea Institute of Oriental Medicine) ;
  • Yang, Hye Jin (KM Application Center, Korea Institute of Oriental Medicine) ;
  • Ma, Jin Yeul (KM Application Center, Korea Institute of Oriental Medicine)
  • 투고 : 2017.12.12
  • 심사 : 2018.02.12
  • 발행 : 2018.03.31
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초록

Acer tegmentosum Maxim (ATM) has been used to treat hepatic disorders in traditional oriental medicine. However, there is little information about phytochemical constituents for quality control of ATM. In this study, we developed and established a simultaneous analytical method of the 10 marker compounds (three coumarins, 3 flavonoids, 1 lignan, 3 phenolics) in ATM using ultra-performance liquid chromatography-mass spectrometry (UPLC-MS/MS). Chromatographic separation of ten target analytes was achieved with a Waters Acquity UPLC BEH $C_{18}$ analytical column ($2.1{\times}100mm$, $1.7{\mu}m$), using a mobile phase of 0.1% (v/v) formic acid in water and acetonitrile with gradient elution. Identifications and quantitation of all analytes were performed using a Q-Exactive UPLC-MS/MS system. Correlation coefficients of the calibration curve for all analytes were ${\geq}0.9986$. The values of limits of detection and quantification of all analytes were 0.5-10.0 and 5.0-50.0 ng/mL, respectively. The established UPLC-MS/MS method successfully identified all target analytes in ATM, and the phytochemicals were 0.01-67.98 mg/g in its lyophilized water extract.

키워드

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