Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Changes in Water Quality and Bacterial Compositions in Culture Water of an Ozonated Flounder Farm

오존 처리한 넙치 양식장 사육수의 수질과 미생물 변동

  • Park, Seongdeok (Gangwon Province Fisheries Resources Institute) ;
  • Kim, You Hee (Department of Aquaculture and Seafood, Gangwon State University) ;
  • Park, Jeonghwan (Department of Marine Bio-Materials and Aquaculture, Pukyong National University) ;
  • Kim, Pyong-Kih (Department of Aquaculture and Seafood, Gangwon State University)
  • 박성덕 (강원도수산자원연구원) ;
  • 김유희 (강원도립대학교 해양양식식품과) ;
  • 박정환 (부경대학교 해양바이오신소재학과) ;
  • 김병기 (강원도립대학교 해양양식식품과)
  • Received : 2017.09.25
  • Accepted : 2018.03.19
  • Published : 2018.03.31
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Abstract

This study assessed the effect of ozone to control pathogenic bacteria in inlet water flowing to flounder farms, establishing operational parameters of ozonation at seawater conditions. Hydraulic retention time in a reaction pipeline after ozonation was fixed at 3 minutes in a flow through system. Concentrations of total residual oxidant (TRO) by ozonation were measured according to different ozonation intensities. The oxidant reduction potential (ORP), which is indirect but enables real-time measurement, was measured in relation to TRO values. TRO values were $0.01{\pm}0.01mg\;L^{-1}$ at an ORP range of 320-410 mV, $0.07{\pm}0.02mg\;L^{-1}$ at 600 mV, and $0.16{\pm}0.03mg\;L^{-1}$ at 700 mV. A heterotrophic marine bacteria colony was reduced by 80.6-97.9%, showing the suppression effect of ozonation on total bacteria in inlet water. At an ORP range of 400-500 mV, colonies of heterotrophic marine bacteria, Vibrio spp., and gram negative bacteria were significantly reduced in outlet water from a culture tank with ongrowing flounder (230 g) at a stocking density of $8kg\;m^{-2}$. Especially, Vibrio spp. and gram negative bacteria were seldom found at 400-500 mV. The daily feeding rate was from over 0.7% to total body weight at 300-500 mV, showing better performance than that in the control. The pathogenic bacteria entering the flounder farm were effectively removed when the ORP range to 400 mV or less.

본 연구는 넙치 양식장 시설 내로 유입되는 병원 미생물을 효과적으로 관리하기 위한 오존 처리 기준을 마련하고자 수행되었다. 유수식 환경에서 오존 주입량을 $0.3{\sim}3.0mg\;O_3L^{-1}$의 범위로 주입하면서 3분의 반응시간을 가진 후 ORP 320~410 mV에서 TRO가 $0.01{\pm}0.01mg\;L^{-1}$가 검출되었으며, 600 mV와 700 mV에서 $0.07{\pm}0.02mg\;L^{-1}$와 $0.16{\pm}0.03mg\;L^{-1}$가 검출되었다. 오존 처리에 따라 총세균수는 80.6~97.9%의 제거율을 나타내어 오존 처리를 통한 미생물 제거효과는 분명하게 나타났다. 230 g 넙치를 $8.0kg\;m^{-2}$ 밀도로 사육한 300~400 mV 실험구에서는 총세균수는 가장 낮은 값을 보였다. 특히 400~500 mV에서 Vibrio spp.와 그램음성균은 거의 검출되지 않았다. 일간사료섭취율은 300~500 mV에서 0.7% 이상을 나타내어 대조구인 자연해수보다 감소하지 않아 부작용이 적은 것으로 나타났다. 넙치 양식장으로 유입되는 병원 미생물은 유입수의 ORP 400 mV 수준에서 효율적으로 제거되는 것으로 조사되었다.

Keywords

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