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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Human amnion-derived mesenchymal stem cells induced osteogenesis and angiogenesis in human adipose-derived stem cells via ERK1/2 MAPK signaling pathway

  • Wang, Yuli (Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University) ;
  • Chen, Xichen (Analysis Center, Nanjing Medical University) ;
  • Yin, Ying (Nanjing Stomatological Hospital, Medical School of Nanjing University) ;
  • Li, Song (Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University)
  • Received : 2018.01.11
  • Accepted : 2018.02.06
  • Published : 2018.04.30
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Abstract

Mesenchymal stem cells (MSCs) have shown great potential in treating bone deficiency. Human adipose-derived stem cells (HASCs) are multipotent progenitor cells with multi-lineage differentiation potential. Human amnion-derived mesenchymal stem cells (HAMSCs) are capable of promoting osteogenic differentiation of MSCs. In this study, we investigated the effect of HAMSCs on HASCs by a transwell co-culture system. HAMSCs promoted proliferation, osteogenic differentiation, angiogenic potential and adiponectin (APN) secretion of HASCs. Moreover, the positive effect of HAMSCs was significantly inhibited by U0126, a highly selective inhibitor of extracellular signaling-regulated kinase 1/2 (ERK1/2) mitogen-activated protein kinase (MAPK) signaling pathway. These observations suggested that HAMSCs induced bone regeneration in HASCs via ERK1/2 MAPK signaling pathway.

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References

  1. Kassebaum NJ, Bernabe E, Dahiya M, Bhandari B, Murray CJ and Marcenes W (2014) Global burden of severe periodontitis in 1990-2010: a systematic review and meta-regression. J Dent Res 93, 1045-1053 https://doi.org/10.1177/0022034514552491
  2. Liu Y, Sun H, Hu M et al (2017) Human Corneal Endothelial Cells Expanded In Vitro Are a Powerful Resource for Tissue Engineering. Int J Med Sci 14, 128-135 https://doi.org/10.7150/ijms.17624
  3. Zuk PA, Zhu M, Ashjian P et al (2002) Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell 13, 4279-4295 https://doi.org/10.1091/mbc.e02-02-0105
  4. Hong JM, Kim BJ, Shim JH et al (2012) Enhancement of bone regeneration through facile surface functionalization of solid freeform fabrication-based three-dimensional scaffolds using mussel adhesive proteins. Acta Biomater 8, 2578-2586 https://doi.org/10.1016/j.actbio.2012.03.041
  5. Park KH, Kim H, Moon S and Na K (2009) Bone morphogenic protein-2 (BMP-2) loaded nanoparticles mixed with human mesenchymal stem cell in fibrin hydrogel for bone tissue engineering. J Biosci Bioeng 108, 530-537 https://doi.org/10.1016/j.jbiosc.2009.05.021
  6. Sheridan RL and Moreno C (2001) Skin substitutes in burns. Burns 27, 92 https://doi.org/10.1016/S0305-4179(00)00076-0
  7. Alviano F, Fossati V, Marchionni C et al (2007) Term Amniotic membrane is a high throughput source for multipotent Mesenchymal Stem Cells with the ability to differentiate into endothelial cells in vitro. BMC Dev Biol 7, 11 https://doi.org/10.1186/1471-213X-7-11
  8. Wolbank S, Peterbauer A, Fahrner M et al (2007) Dose-dependent immunomodulatory effect of human stem cells from amniotic membrane: a comparison with human mesenchymal stem cells from adipose tissue. Tissue Eng 13, 1173-1183 https://doi.org/10.1089/ten.2006.0313
  9. Wang Y, Yin Y, Jiang F and Chen N (2015) Human amnion mesenchymal stem cells promote proliferation and osteogenic differentiation in human bone marrow mesenchymal stem cells. J Mol Histol 46, 13-20 https://doi.org/10.1007/s10735-014-9600-5
  10. Wang Y, Jiang F, Liang Y, Shen M and Chen N (2016) Human Amnion-Derived Mesenchymal Stem Cells Promote Osteogenic Differentiation in Human Bone Marrow Mesenchymal Stem Cells by Influencing the ERK1/2 Signaling Pathway. Stem Cells Int 2016, 4851081
  11. Wang Y, Ma J, Du Y, Miao J and Chen N (2016) Human Amnion-Derived Mesenchymal Stem Cells Protect Human Bone Marrow Mesenchymal Stem Cells against Oxidative Stress-Mediated Dysfunction via ERK1/2 MAPK Signaling. Mol Cells 39, 186-194 https://doi.org/10.14348/molcells.2016.2159
  12. Wang Y, Wu H, Shen M, Ding S, Miao J and Chen N (2017) Role of human amnion-derived mesenchymal stem cells in promoting osteogenic differentiation by influencing p38 MAPK signaling in lipopolysaccharide-induced human bone marrow mesenchymal stem cells. Exp Cell Res 350, 41-49 https://doi.org/10.1016/j.yexcr.2016.11.003
  13. Lee HW, Kim SY, Kim AY, Lee EJ, Choi JY and Kim JB (2009) Adiponectin stimulates osteoblast differentiation through induction of COX2 in mesenchymal progenitor cells. Stem Cells 27, 2254-2262 https://doi.org/10.1002/stem.144
  14. Jiang X, Song D, Ye B et al (2011) Effect of intermittent administration of adiponectin on bone regeneration following mandibular osteodistraction in rabbits. J Orthop Res 29, 1081-1085 https://doi.org/10.1002/jor.21355
  15. Park M, Wu D, Park T et al (2013) APPL1 transgenic mice are protected from high-fat diet-induced cardiac dysfunction. Am J Physiol Endocrinol Metab 305, E795-804 https://doi.org/10.1152/ajpendo.00257.2013
  16. Corbella S, Taschieri S, Francetti L, Weinstein R and Del Fabbro M (2017) Histomorphometric Results After Postextraction Socket Healing with Different Biomaterials: A Systematic Review of the Literature and Meta-Analysis. Int J Oral Maxillofac Implants 32, 1001-1017 https://doi.org/10.11607/jomi.5263
  17. Gimble JM, Katz AJ and Bunnell BA (2007) Adiposederived stem cells for regenerative medicine. Circ Res 100, 1249-1260 https://doi.org/10.1161/01.RES.0000265074.83288.09
  18. Tsuji W, Rubin JP and Marra KG (2014) Adipose-derived stem cells: Implications in tissue regeneration. World J Stem Cells 6, 312-321 https://doi.org/10.4252/wjsc.v6.i3.312
  19. Ruetze M and Richter W (2014) Adipose-derived stromal cells for osteoarticular repair: trophic function versus stem cell activity. Expert Rev Mol Med 16, e9 https://doi.org/10.1017/erm.2014.9
  20. Kempen DH, Lu L, Heijink A et al (2009) Effect of local sequential VEGF and BMP-2 delivery on ectopic and orthotopic bone regeneration. Biomaterials 30, 2816-2825 https://doi.org/10.1016/j.biomaterials.2009.01.031
  21. Kanczler JM and Oreffo RO (2008) Osteogenesis and angiogenesis: the potential for engineering bone. Eur Cell Mater 15, 100-114 https://doi.org/10.22203/eCM.v015a08
  22. Carano RA and Filvaroff EH (2003) Angiogenesis and bone repair. Drug Discov Today 8, 980-989 https://doi.org/10.1016/S1359-6446(03)02866-6
  23. Hsiao ST, Asgari A, Lokmic Z et al (2012) Comparative analysis of paracrine factor expression in human adult mesenchymal stem cells derived from bone marrow, adipose, and dermal tissue. Stem Cells Dev 21, 2189-2203 https://doi.org/10.1089/scd.2011.0674
  24. Konig J, Huppertz B, Desoye G et al (2012) Amnionderived mesenchymal stromal cells show angiogenic properties but resist differentiation into mature endothelial cells. Stem Cells Dev 21, 1309-1320 https://doi.org/10.1089/scd.2011.0223
  25. Fierro FA, O'Neal AJ, Beegle JR et al (2015) Hypoxic pre-conditioning increases the infiltration of endothelial cells into scaffolds for dermal regeneration pre-seeded with mesenchymal stem cells. Front Cell Dev Biol 3, 68
  26. Oshima K, Nampei A, Matsuda M et al (2005) Adiponectin increases bone mass by suppressing osteoclast and activating osteoblast. Biochem Biophys Res Commun 331, 520-526 https://doi.org/10.1016/j.bbrc.2005.03.210
  27. Williams GA, Wang Y, Callon KE et al (2009) In vitro and in vivo effects of adiponectin on bone. Endocrinology 150, 3603-3610 https://doi.org/10.1210/en.2008-1639
  28. Yamaguchi N, Kukita T, Li YJ et al (2008) Adiponectin inhibits induction of TNF-alpha/RANKL-stimulated NFATc1 via the AMPK signaling. FEBS Lett 582, 451-456 https://doi.org/10.1016/j.febslet.2007.12.037
  29. Chen T, Wu YW, Lu H, Guo Y and Tang ZH (2015) Adiponectin enhances osteogenic differentiation in human adipose-derived stem cells by activating the APPL1-AMPK signaling pathway. Biochem Biophys Res Commun 461, 237-242 https://doi.org/10.1016/j.bbrc.2015.03.168
  30. Li S, Huang L, Sun Y et al (2015) Slit2 Promotes Angiogenic Activity Via the Robo1-VEGFR2-ERK1/2 Pathway in Both In Vivo and In Vitro Studies. Invest Ophthalmol Vis Sci 56, 5210-5217 https://doi.org/10.1167/iovs-14-16184
  31. Hammond CL and Schulte-Merker S (2009) Two populations of endochondral osteoblasts with differential sensitivity to Hedgehog signalling. Development 136, 3991-4000 https://doi.org/10.1242/dev.042150
  32. Krum SA, Chang J, Miranda-Carboni G and Wang CY (2010) Novel functions for NFkappaB: inhibition of bone formation. Nat Rev Rheumatol 6, 607-611 https://doi.org/10.1038/nrrheum.2010.133
  33. Hu N, Feng C, Jiang Y, Miao Q and Liu H (2015) Regulative Effect of Mir-205 on Osteogenic Differentiation of Bone Mesenchymal Stem Cells (BMSCs): Possible Role of SATB2/Runx2 and ERK/MAPK Pathway. Int J Mol Sci 16, 10491-10506 https://doi.org/10.3390/ijms160510491
  34. Franceschi RT and Xiao G (2003) Regulation of the osteoblast-specific transcription factor, Runx2: responsiveness to multiple signal transduction pathways. J Cell Biochem 88, 446-454 https://doi.org/10.1002/jcb.10369
  35. Zhang D, Jiang M and Miao D (2011) Transplanted human amniotic membrane-derived mesenchymal stem cells ameliorate carbon tetrachloride-induced liver cirrhosis in mouse. PLoS One 6, e16789 https://doi.org/10.1371/journal.pone.0016789
  36. Chen YJ, Chung MC, Jane Yao CC et al (2012) The effects of acellular amniotic membrane matrix on osteogenic differentiation and ERK1/2 signaling in human dental apical papilla cells. Biomaterials 33, 455-463 https://doi.org/10.1016/j.biomaterials.2011.09.065
  37. Kim KI, Park S and Im GI (2014) Osteogenic differentiation and angiogenesis with cocultured adipose-derived stromal cells and bone marrow stromal cells. Biomaterials 35, 4792-4804 https://doi.org/10.1016/j.biomaterials.2014.02.048

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