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Effect of carbohydrase treatments on phenolics content and antioxidant activity of maize flour

탄수화물 가수분해효소 처리가 옥수수 가루의 페놀산과 항산화활성에 미치는 영향

  • Cho, Dong-Hwa (Department of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Mi Jung (Research Policy Bureau, Rural Development Administration) ;
  • Sim, Eun-Yeong (Department of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration) ;
  • Jeon, Yong Hee (Department of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration) ;
  • Lee, Choon Ki (Department of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration) ;
  • Woo, Koan Sik (Department of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration)
  • 조동화 (농촌진흥청 국립식량과학원 중부작물부) ;
  • 김미정 (농촌진흥청 연구정책국) ;
  • 심은영 (농촌진흥청 국립식량과학원 중부작물부) ;
  • 전용희 (농촌진흥청 국립식량과학원 중부작물부) ;
  • 이춘기 (농촌진흥청 국립식량과학원 중부작물부) ;
  • 우관식 (농촌진흥청 국립식량과학원 중부작물부)
  • Received : 2017.11.30
  • Accepted : 2018.01.03
  • Published : 2018.04.30

Abstract

Enzymatic treatments of maize flour (MF) were investigated using commercial carbohydrases (Ultraflo L and Pentopan 500 BG) to enhance the phenolic acid content and antioxidant property. The total phenolic acid content of the MF was 3.76 mg/100 g, whereas those of the Pentopan 500 BG and Ultraflo L treated MF were 6.85 and 39.55 mg/100 g, respectively. Particularly, ferulic acid content of Pentopan 500 BG-treated MF was 20.0 times higher than that of untreated MF (1.7 vs. 33.9 mg/100 g). Pentopan 500 BG appeared to be more effective than Ultraflo L in increasing the free phenolic acid content. Antioxidant activities of enzyme treated MF were significantly higher than untreated MF. In particular, the Pentopan 500 BG-treated MF (16.0 mmol TE/100 g) was approximately 1.5 times higher than untreated MF (12.6 mmol TE/100 g). Enzymatic hydrolysis of cell wall polysaccharides in MF could be used as an effective procedure for not only increasing phenolic content but also antioxidant activities.

본 연구에서는 탄수화물 가수분해효소가 옥수수 가루의 페놀 화합물 및 항산화성에 미치는 영향을 조사하였다. 옥수수 가루는 빵, 과자 등 다양한 가공 식품 뿐만 아니라 가축용 사료로도 이용되고 있으며, 이에 대한 기능성의 증가는 국민 및 반려동물의 건강성 증진에 기여할 수 있을 것이다. 본 실험에서 탄수화물 가수분해효소 처리는 옥수수 가루의 유리 페놀산 및 총 페놀 화합물 함량을 유의적으로 증가시켰으며, 이는 효소 처리가 세포벽 구성성분과 강하게 결합하고 있는 불용성 페놀산을 유리화하기 때문이다. 또한 옥수수 가루의 항산화활성 또한 효소처리에 의해 유의적으로 증가하였는데, 이는 강한 항산화활성을 가진 유리 페놀산이 탄수화물 가수분해효소에 의해 증가하였기 때문이다. 상용 탄수화물 가수분해효소 처리는 친환경적일 뿐만 아니라 공정이 단순하여 상업적으로 이용하기 쉬울 것이라 생각된다. 하지만 아직 탄수화물 가수분해효소 처리가 옥수수 가루의 가공 적성에 미치는 영향 및 생산법 최적화에 대한 연구가 이루어져 있지 않으며 이에 대한 추가적인 연구가 필요할 것이다.

Keywords

References

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