Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Antioxidative and Inhibitory Activities of Extract and Juice Powder from Hippophae rhamnoides L. against Nitric Oxide and Elastase Production

비타민나무 추출물 및 착즙 분말의 항산화 활성 및 Nitric Oxide 생성과 Elastase에 대한 저해활성

  • Park, Ju Hee (Institute of Samsung Herb Medicine) ;
  • Lee, Chan Ok (Bioherb Research Institute, Kangwon National University) ;
  • Yoo, Ji Hye (Bioherb Research Institute, Kangwon National University) ;
  • An, Nguyen Thi Lan (Department of Bio-Resource Sciences, Kangwon National University) ;
  • Yoo, Nam Ho (Department of Bio-Resource Sciences, Kangwon National University) ;
  • Kim, Myong Jo (Department of Bio-Resource Sciences, Kangwon National University)
  • 박주희 (삼성생약(주)부설 바이오생명공학연구소) ;
  • 이찬옥 (강원대학교 한방바이오연구소) ;
  • 유지혜 (강원대학교 한방바이오연구소) ;
  • ;
  • 유남호 (강원대학교 생물자원과학과) ;
  • 김명조 (강원대학교 생물자원과학과)
  • Received : 2018.02.06
  • Accepted : 2018.04.05
  • Published : 2018.04.30
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Abstract

Background: The flowering plant Hippophae rhamnoides L. has been used for many studies on fruit or leaf extracts. This study was conducted to investigate the development of a new cosmetic material from H. rhamnoides fruits and leaves that have by antioxidant, anti-inflammatory and wrinkle improvement activities. Methods and Results: The antioxidant abilities of H. rhamnoides extracts, including of a water-soluble fruit powder (FW), a fatsoluble fruit powder (FF), a supercritical extract of fruit by-product (BS), and a mixture of leaf and fruit (MIX), were investigated in vitro. A DPPH radical assay for antioxidant activity was performed for these fractions alongside assay to evaluate the total phenolic and flavonoid content (TPC and TFC). As expected, the MIX had the highest DPPH radical scavenging activity ($RC_{50}=10.27{\mu}g/m{\ell}$), and the TPC and TFC also were highest in MIX ($225.7mg{\cdot}GAE/g$, and $25.18mg{\cdot}QE/g$, respectively). Nitric oxide (NO) production in LPS-induced RAW264.7 cells was estimated and the results indicated an over 75% decrease of NO production in FF and MIX. In other assays, the highest elastase inhibitory activity was found in FW. Conclusions: These results revealed that H. rhamnoides extracts have a high potential for antioxidant, anti-inflammatory and antiwrinkle activities. H. rhamnoides products are suggested to be applied as the functional materials of cosmetic ingredients.

Keywords

References

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