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The Effects of Flammulina velutipes Water Extract on the Activation of Spleen Cell and Macrophage in Mice

팽이버섯 열수추출물이 마우스 비장세포와 대식세포의 증식 및 활성에 미치는 효과

  • Kim, Kyoung-Ok (Dept. of Food Service Management and Nutrition, Kongju National University) ;
  • Ryu, Hye-Sook (Dept. of Food and Nutrition, College of Health Sciences, Sangji University)
  • 김경옥 (공주대학교 외식상품학과) ;
  • 류혜숙 (상지대학교 보건과학대학 식품영양학과)
  • Received : 2017.12.18
  • Accepted : 2018.02.06
  • Published : 2018.04.30

Abstract

Flammulina velutipes is an edible mushroom and contains a lot of fiber, vitamin $B_1$, $B_2$, niacin and folic acid. This study was conducted to explore the effects of the Flammulina velutipes mushroom on immune cells and immunity. Th1 cytokine productions as $IFN-{\gamma}$, $TNF-{\alpha}$, and IL-2 were measured in an activated macrophage by Flammulina velutipes water extract in seven concentrations (0, 5, 10, 50, 100, 250, 500, and $1,000{\mu}g/mL$). Also, the splenocyte proliferation index was measured at 48 hours after treatment of the Flammulina velutipes water extract in seven concentrations or mitogen, LPS and ConA. The $IFN-{\gamma}$ and $TNF-{\alpha}$ productions were increased by treatment of the Flammulina velutipes water extract. The $TNF-{\alpha}$ production was significantly higher in the $50{\sim}1,000{\mu}g/mL$ Flammulina velutipes water extract treated macrophages. The $IFN-{\gamma}$ production of macrophages treated with the Flammulina velutipes water extract increased significantly in all groups, and the highest $1000{\mu}g/mL$ concentration. The splenocyte proliferation index was enhanced when the $10{\sim}1,000{\mu}g/mL$ Flammulina velutipes water extracts were treated compared to the control. These primary results suggest that Flammulina velutipes may enhance the immune function by activation of the macrophage and spleen cell.

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