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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Medicarpin induces lipolysis via activation of Protein Kinase A in brown adipocytes

  • Imran, Khan Mohammad (Institute of tissue regeneration, College of Medicine, Soonchunhyang University) ;
  • Yoon, Dahyeon (Institute of tissue regeneration, College of Medicine, Soonchunhyang University) ;
  • Lee, Tae-Jin (Department of Anatomy, College of Medicine, Yeungnam University) ;
  • Kim, Yong-Sik (Institute of tissue regeneration, College of Medicine, Soonchunhyang University)
  • Received : 2017.11.29
  • Accepted : 2018.01.18
  • Published : 2018.05.31
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Abstract

Natural pterocarpan Medicarpin (Med) has been shown to have various beneficial biological roles, including inhibition of osteoclastogenesis, stimulation of bone regeneration and induction of apoptosis. However, the effect of the Med on lipolysis in adipocytes has not been reported. Here, we show the effect of Med on lipolysis in different mouse adipocytes and elucidate the underlying mechanism. We observed that Med treatment promoted release of glycerol in the media. Differentiated mouse brown adipose tissue cells were treated with Med. RNA-Seq analysis was performed to elucidate the effect of med and subsequently was confirmed by qRT-PCR and western blotting analyses. Med treatment increased both protein and gene expression levels of hormone-sensitive lipase (Hsl) and adipose triglyceride lipase (Atgl), which are two critical enzymes necessary for lipolysis. Mechanistic study showed that Med activates Protein Kinase A (PKA) and phosphorylates Hsl at PKA target position at $Serine^{660}$. Silencing of PKA gene by short interfering RNA attenuated the Med-induced increase in glycerol release and Hsl phosphorylation. The results unveil that Med boosts lipolysis via a PKA-dependent pathway in adipocytes and may provide a possible avenue of further research of Med mediated reduction of body fat.

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References

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