Korean Chemical Engineering Research

The Korean Institute of Chemical Engineers (한국화학공학회)
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Decreasing Particle Size of (+)-Dihydromyricetin Using Hydrophilic Polymer in Fractional Precipitation

분별침전에서 친수성 고분자 물질을 이용한 (+)-dihydromyricetin의 입자크기 감소

  • Ji, Seong Bin (Department of Chemical Engineering, Kongju National University) ;
  • Kim, Jin-Hyun (Department of Chemical Engineering, Kongju National University)
  • 지성빈 (공주대학교 화학공학부) ;
  • 김진현 (공주대학교 화학공학부)
  • Received : 2018.02.13
  • Accepted : 2018.05.01
  • Published : 2018.06.01
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Abstract

In this study, we have applied fractional precipitation with hydrophilic polymer in order to decrease the particle size of the (+)-dihydromyricetin from plant materials. When compared with the case where no hydrophilic polymer was employed, the addition of hydrophilic polymer in fractional precipitation resulted in a considerable decrease in the size of the (+)-dihydromyricetin precipitate. Among the polymers used, HPMC 2910 was the most effective for inhibition of precipitate growth. A polymer concentration of 0.1% (w/v) yielded the smallest particle size. The particle size was reduced by ~40% compared to control. In addition, the precipitate size was inversely correlated with the absolute value of the zeta potential of the suspension with polymer.

본 연구에서는 생리활성물질 (+)-dihydromyricetin의 입자크기 감소를 위하여 친수성 고분자 물질을 첨가하여 분별침전을 수행하였다. 고분자 물질(HPMC 2910, PVP K90, PVA)이 첨가된 분별침전을 통해 (+)-dihydromyricetin 입자크기를 감소시킬 수 있었다. 특히 분별침전 시 HPMC 2910 (0.1%, w/v)을 첨가할 경우, 대조군에 비하여 입자크기가 $38-68{\mu}m$ (32-40%) 정도 감소하여 입자크기 감소에 가장 효과적이었다. 또한 (+)-dihydromyricetin침전물의 입자크기는 친수성 고분자 물질 첨가에 따른 침전용액의 제타전위 절대값에 반비례함을 알 수 있었다.

Keywords

References

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