Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Investigation of the Effect of Sappan Lignum and Brazilin on Expression of Tight Junction Related-genes in Human Keratinocyte

소목(蘇木)과 그 지표물질인 brazilin이 인간 유래 각질 형성 세포의 tight junction 유전자 발현에 미치는 영향

  • Cheon, Seong Hye (Department of Biochemistry, Chungnam National University) ;
  • Choi, Sun Kyung (Department of Biochemistry, Chungnam National University) ;
  • Cho, Nam Joon (Department of Biochemistry, Chungnam National University) ;
  • Kim, Kee Kwang (Department of Biochemistry, Chungnam National University) ;
  • Lee, Woong Hee (Institute of Biotechnology, Chungnam National University) ;
  • Hwang, Hyung Seo (Department of Oriental Cosmetic Science, Semyung University) ;
  • Kim, Kyoon Eon (Department of Biochemistry, Chungnam National University) ;
  • Han, Hyosang (Department of Health Administration, College of Social Sciences, Joongbu University)
  • 천성혜 (충남대학교 생화학과) ;
  • 최선경 (충남대학교 생화학과) ;
  • 조남준 (충남대학교 생화학과) ;
  • 김기광 (충남대학교 생화학과) ;
  • 이웅희 (충남대학교 생물공학연구소) ;
  • 황형서 (세명대학교 한방화장품과학과) ;
  • 김균언 (충남대학교 생화학과) ;
  • 한효상 (중부대학교 보건행정학과)
  • Received : 2017.12.26
  • Accepted : 2018.03.06
  • Published : 2018.04.25
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Abstract

The aim of this research was to determine the diverse effects of Sappan Lignum extract and brazilin on human keratinocyte HaCaT cells. We confirmed the antioxidant effect of Sappan Lignum extract and brazilin was analyzed by using an ABTS assay, confirming the efficacy of water extraction method. Also, we examined effect of Sappan Lignum extract and brazilin on the cell viability, using the MTS assay in HaCaT cells. mRNA expression levels of tight junction-related genes associated with skin barrier in HaCaT cells were analyzed using quantitative real-time PCR analysis. Sappan Lignum extract increased the cellular activity of HaCaT cells and the expression of the tight junction-related genes claudin 3, claudin 6, and ZO-2. Brazilin displayed the same effects as that of the extract on HaCaT cells activity and tight junction-related genes expression. Furthermore, dispase assay demonstrated altered cell-cell adhesion strength of Sappan Lignum extract or brazilin treated HaCaT cells. Sappan Lignum extract or brazilin might be an useful ingredient in skin-mosturizinng and anti-wrinkle cosmetics, given its effects of altering mRNA expression of tight junction-related genes and enhancing cell-cell adhesion strength of HaCaT cells.

Keywords

References

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