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Verification of Biological Activities and Tyrosinase Inhibition of Ethanol Extracts from Hemp Seed (Cannabis sativa L.) Fermented with Lactic Acid Bacteria

대마씨 발효 추출물의 생리 활성 및 미백 활성 검증

  • Yoon, Yeo-Cho (BHNBIO Co., LTD, Institute for Developments of BioIndustrial-Materials) ;
  • Kim, Byung-Hyuk (BHNBIO Co., LTD, Institute for Developments of BioIndustrial-Materials) ;
  • Kim, Jung-Kyu (BHNBIO Co., LTD, Institute for Developments of BioIndustrial-Materials) ;
  • Lee, Jun-Hyeong (BHNBIO Co., LTD, Institute for Developments of BioIndustrial-Materials) ;
  • Park, Ye-Eun (BHNBIO Co., LTD, Institute for Developments of BioIndustrial-Materials) ;
  • Kwon, Gi-Seok (Department of Medicinal Plant Resources, Andong National University) ;
  • Hwang, Hak Soo (Kyochon F&B) ;
  • Lee, Jung-Bok (BHNBIO Co., LTD, Institute for Developments of BioIndustrial-Materials)
  • 윤여초 (비에이치앤바이오 기업 부설 생물산업소재개발연구소) ;
  • 김병혁 (비에이치앤바이오 기업 부설 생물산업소재개발연구소) ;
  • 김중규 (비에이치앤바이오 기업 부설 생물산업소재개발연구소) ;
  • 이준형 (비에이치앤바이오 기업 부설 생물산업소재개발연구소) ;
  • 박예은 (비에이치앤바이오 기업 부설 생물산업소재개발연구소) ;
  • 권기석 (안동대학교 생약자원학과) ;
  • 황학수 (교촌F&B(주)) ;
  • 이중복 (비에이치앤바이오 기업 부설 생물산업소재개발연구소)
  • Received : 2017.11.20
  • Accepted : 2018.06.27
  • Published : 2018.06.30

Abstract

Hemp seed (Cannabis sativa L.; HS), an annual herbaceous plant in the Cannabis genus, has been reported to play various biological functions in immunity increase, atherosclerosis, constipation, hyperlipidemia prevention, anti-inflammatory, and anti-cancer. In recently years, as superfood, the growing interest in the health care benefits of hemp seed has led to increased consumption. In this study, we investigated the effect of an ethanol extract of HS fermented with lactic acid bacteria (Lactobacillus plantarum KCTC 3107, L. plantarum KCTC 3108, L. brevis BHN-LAB128, L. paracasei BHN-LAB129). An antibacterial activity against Staphylococcus aureus and Bacillus cereus were 13.99 mm and 15.17 mm, respectively. The ethanol extracts of fermented hemp seed by lactic acid bacteria that the contents of total polyphenol, total flavonoid content, DPPH radical scavenging activity, SOD-like activity, and ${\alpha}$-glucosidase inhibitory activity were increased compared to non-fermented hemp seed. Also, tyrosinase inhibitory activity of the fermented hemp seed (FHS), known to melanin increasing substance was increased. In these results, we suggested that FHS have effects of anti-oxidant, ${\alpha}$-glucosidase inhibitory activity, and tyrosinase inhibitory activity. Hence, we proposed that FHS has possible to development as functional foods and cosmetics.

대마씨(Hemp seed; seed of Cannabis sativa L.)는 삼과에 속하는 1년생 초본 식물이며, 면역력 증가, 동맥 경화증, 변비, 고지혈증 예방, 항염증제, 항암제 등 다양한 생물학적 기능을 수행하는 것으로 보고되었다. 본 연구에서는 유산균을 이용한 발효 대마씨 추출물의 효능를 조사하였다. 그 결과, Staphylococcus aureus에 대한 항균 활성은 발효하지 않은 대마씨 추출물에 비해 현저히 증가되었으며, 특히 Bacillus cereus에 대하여 발효한 대마씨 추출물에서 항균 활성이 새롭게 나타났다. 또한, 유산균 발효 대마씨 추출물의 총 폴리페놀 함량과 DPPH 라디칼 소거능, SOD 유사 활성, ${\alpha}$-glucosidase 저해 활성은 발효하지 않은 대마씨 추출물에 비해 각각 증가됨을 확인하였다. 추가적으로 멜라닌 증가 물질로 알려진 tyrosinase의 저해 활성도 발효하지 않은 유산균에 비해 증가되었다. 이러한 결과들을 통해 유산균으로 발효한 대마씨 추출물은 항산화, ${\alpha}$-glucosidase 저해 활성 및 tyrosinase 저해 활성을 촉진시키며, 따라서 유산균으로 발효한 대마씨 추출물을 이용한 기능성 소재 및 식품 개발로의 활용이 가능할 것으로 기대한다.

Acknowledgement

Supported by : 안동대학교

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