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Opisthorchis felineus and Metorchis bilis Metacercariae in Cyprinid Fish Leuciscus idus in Nura-Sarysu River, Kazakhstan

  • Kiyan, Vladimir S. (Department of Microbiology and Biotechnology, Saken Seifullin Kazakh Agrotechnical University) ;
  • Bulashev, Aitbay K. (Department of Microbiology and Biotechnology, Saken Seifullin Kazakh Agrotechnical University) ;
  • Katokhin, Aleksey V. (Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences)
  • 투고 : 2017.11.06
  • 심사 : 2018.05.28
  • 발행 : 2018.06.30

초록

Aim of the present study was to provide presence of opisthorchiid metacercariae in cyprinid fish Leuciscus idus in Nura-Sarysu river, Kazakhstan. Infection rate of the ides by the metacercariae was 42%. The metacercariae, similar morphologically to those of the liver flukes, were found: elliptical in shape, $0.19-0.25{\times}0.15-0.22mm$, oral and ventral suckers nearly equal size, and excretory bladder O-shape with black content, occupying posterior part of the body. The metacercariae were divided into 2 groups with differences in size and thickness of cyst wall. Adult flukes were recovered from the Syrian hamsters infected with the opisthorch metacercariae and identified with morphological characters to Opisthorchis felineus and Metorchis bilis. DNA sequences of ITS1, ITS2, and cox1 supported the taxonomic assignment.

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참고문헌

  1. IARC. Infection with liver flukes (Opisthorchis viverrini, Opisthorchis felineus and Clonorchis sinensis). IARC Monogr Eval Carcinog Risks Hum1994; 61: 121-175.
  2. Sripa B, Bethony JM, Sithithaworn P, Kaewkes S, Mairiang E, Loukas A, Mulvenna J, Laha T, Hotez PJ, Brindley PJ. Opisthorchiasis and Opisthorchis - associated cholangiocarcinoma in Thailand and Laos. Acta Trop 2011; 120: 158-168. https://doi.org/10.1016/j.actatropica.2010.07.006
  3. Armignacco O, Ferri F, Gomez-Morales MA, Caterini L, Pozio E. Cryptic and asymptomatic Opisthorchis felineus infections. Am J Trop Med Hyg 2013; 88: 364-366. https://doi.org/10.4269/ajtmh.2012.12-0280
  4. Chai JY. Epidemiology of trematode infections. In Toledo R, Fried B eds, Digenetic Trematodes. New York, USA. Springer. 2014, pp 241-292.
  5. Fedorova OS, Kovshirina YV, Kovshirina AE, Fedotova MM, Deev IA, Petrovskiy FI, Filimonov AV, Dmitrieva AI, Kudyakov LA, Saltykova IV, Odermatt P, Ogorodova LM. Opisthorchis felineus infection and cholangiocarcinoma in the Russian Federation: a review of medical statistics. Parasitol Int 2016; 66: 365-371.
  6. King S, Scholz T. Trematodes of the family Opisthorchiidae: a minireview. Korean J Parasitol 2001; 39: 209-221. https://doi.org/10.3347/kjp.2001.39.3.209
  7. Kaewkes S. Taxonomy and biology of liver flukes. Acta Trop 2003; 88: 177-186. https://doi.org/10.1016/j.actatropica.2003.05.001
  8. Beer SA. Biology of opisthorchosis invasion agent. 1st ed. Moscow. 2005, pp 115-164.
  9. Kaewpitoon N, Kootanavanichpong N, Kompor P, Chavenkun W, Kujapun J, Norkaew J, Ponphimai S, Matrakool L, Tongtawee T, Panpimanmas S, Rujirakul R, Padchasuwan N, Pholsripradit P, Eksanti T, Phatisena T, Loyd RA, Kaewpitoon SJ. Review and Current Status of Opisthorchis viverrini Infection at the Community Level in Thailand. Asian Pac J Cancer Prev 2015; 16: 6825-6830. https://doi.org/10.7314/APJCP.2015.16.16.6825
  10. Tang ZL, Huang Y, Yu XB. Current status and perspectives of Clonorchis sinensis and clonorchiasis: epidemiology, pathogenesis, omics, prevention and control. Infect Dis Poverty 2016; 5: 71. https://doi.org/10.1186/s40249-016-0166-1
  11. Mordvinov VA, Yurlova NI, Ogorodova LM, Katokhin AV. Opisthorchis felineus and Metorchis bilis are the main agents of liver fluke infection of humans in Russia. Parasitol Int 2012; 61: 25-31. https://doi.org/10.1016/j.parint.2011.07.021
  12. Schuster RK. Opisthorchiidosis--a review. Infec Disord Drug Targets 2010; 10: 402-415. https://doi.org/10.2174/187152610793180902
  13. Pozio E, Armignacco O, Ferri F, Gomez Morales MA. Opisthorchis felineus, an emerging infection in Italy and its implication for the European Union. Acta Trop 2013; 126: 54-62. https://doi.org/10.1016/j.actatropica.2013.01.005
  14. WHO. Control of food borne trematode infections. Report of a WHO study group. World Health Organ Tech Rep Ser 1995; 849: 1-157.
  15. Sultanov A, Abdybekova A, Abdibaeva A, Shapiyeva Z, Yeshmuratov T, Torgerson PR. Epidemiology of fish-borne trematodiasis in Kazakhstan. Acta Trop 2014; 138: 60-66. https://doi.org/10.1016/j.actatropica.2014.04.030
  16. Kuznetsova VG, Naumov VA, Belov GF. Methorchiasis in the residents of Novosibirsk area, Russia. Cytobios 2000; 102: 33-34.
  17. Fedorov KP, Naumov VA, Kuznetsova VG, Belov GF. Some real problems of human opisthorchiasis. Med Parazitol (Mosk) 2002; 3: 7-9 (in Russian).
  18. Teimoori S, Arimatsu Y, Laha T, Kaewkes S, Sereerak P, Sripa M, Tangkawattana S, Brindley PJ, Sripa B. Chicken IgY-based coproantigen capture ELISA for diagnosis of human opisthorchiasis. Parasitol Int 2017; 66: 443-447. https://doi.org/10.1016/j.parint.2015.10.011
  19. Bulashev AK, Borovikov SN, Serikova SS, Suranshiev ZA, Kiyan VS, Eskendirova SZ. Development of an ELISA using anti-idiotypic antibody for diagnosis of opisthorchiasis. Folia Parasitol (Praha) 2016; 63: 025.
  20. Waikagul J, Dekumyoy P, Chaichana K, Thairungroje Anantapruti M, Komalamisra C, Kitikoon V. Serodiagnosis of human opisthorchiasis using cocktail and electroeluted Bithynia snail antigens. Parasitol Int 2002; 51: 237-247. https://doi.org/10.1016/S1383-5769(02)00013-2
  21. Starkova TV, Poletaeva OG, Kovrova EA, Krasovskaia NN, Tkachenko TN, Masiago AV, Ofitserov VI, Tereshchenko AIu. The efficiency of the enzyme immunoassay test system opisthorchiasis-CIC-EIA-best to detect circulating immune complexes containing opisthorchis antigens in the serum of patients with opisthorchiasis. Med Parazitol (Mosk) 2011; 3: 44-45 (in Russian).
  22. Nockler K, Dell K, Schuster R, Voigt WP. Indirect ELISA for the detection of antibodies against Opisthorchis felineus (Rivolta, 1884) and Metorchis bilis (Braun, 1790) in foxes. Vet Parasitol 2003; 110: 207-215. https://doi.org/10.1016/S0304-4017(02)00324-2
  23. Muller B, Schmidt J, Mehlhorn H. PCR diagnosis of infections with different species of Opisthorchiidae using a rapid clean-up procedure for stool samples and specific primers. Parasitol Res 2007; 100: 905-909. https://doi.org/10.1007/s00436-006-0321-x
  24. Aung WPP, Htoon TT, Tin HH, Thinn KK, Sanpool O, Jongthawin J, Sadaow L, Phosuk I, Rodpai R, Intapan PM, Maleewong W. First report and molecular identification of Opisthorchis viverrini infection in human communities from Lower Myanmar. PLoS One 2017; 12: e0177130. https://doi.org/10.1371/journal.pone.0177130
  25. Sahu R, Biswal DK, Roy B, Tandon V. Molecular characterization of Opisthorchis noverca (Digenea: Opisthorchiidae) based on nuclear ribosomal ITS2 and mitochondrial CO1 genes. J Helminthol 2016; 90: 607-614. https://doi.org/10.1017/S0022149X15000851
  26. Brusentsov II, Katokhin AV, Sakharovskaia ZV, Sazonov AE, Ogorodova LM, Fedorova OS, Kolchanov NA, Mordvinov VA. DNA diagnosis of mixed invasions of Opisthorchis felineus and Metorchis bilis by polymerase chain reaction. Med Parazitol (Mosk) 2010; 2: 10-13 (in Russian).
  27. Pavliukobv IA, Berezantsev IuA. The ethyl acetate method of studying feces for Opisthorchis eggs. Med Parazitol (Mosk) 1991; 3: 57 (in Russian).
  28. Boom R, Sol CJ, Salimans MM, Jansen CL, Wertheim-van Dillen PM, van der Noordaa J. Rapid and simple method for purification of nucleic acids. J Clin microbial 1990; 28: 495-503.
  29. Muller B, Schmidt J, Mehlhorn H. Sensitive and species-specific detection of Clonorchis sinensis by PCR in infected snails and fishes. Parasitol Res 2007; 100: 911-914. https://doi.org/10.1007/s00436-006-0322-9
  30. Kang S, Sultana T, Loktev VB, Wongratanacheewin S, Sohn WM, Eom KS, Park JK. Molecular identification and phylogenetic analysis of nuclear rDNA sequences among three opisthorchid liver fluke species (Opisthorchiidae: Trematoda). Parasitol Int 2008; 57: 191-197. https://doi.org/10.1016/j.parint.2007.12.007
  31. Katokhin AV, Shekhovtsov SV, Konkow S, Yurlova NI, Serbina EA, Vodianitskai SN, Fedorov KP, Loktev VB, Muratov IV, Ohyama F, Makhnev TV, Pel'tek SE, Mordvinov VA. Assessment of the genetic distinctions of Opisthorchis felineus from O. viverrini and Clonorchis sinensis by ITS2 and CO1 sequences. Dokl Biochem Biophys 2008; 421: 214-217. https://doi.org/10.1134/S1607672908040133
  32. Brusentsov II, Katokhin AV, Brusentsova IV, Shekhovtsov SV, Borovikov SN, Goncharenko GG, Lider LA, Romashov BV, Rusinek OT, Shibitov SK, Suleymanov MM, Yevtushenko AV, Mordvinov VA. Low genetic diversity in wide-spread Eurasian liver fluke Opisthorchis felineus suggests special demographic history of this trematode species. PLoS One 2013; 8: e62453. https://doi.org/10.1371/journal.pone.0062453
  33. Pauly A, Schuster R, Steuber S. Molecular characterization and differentiation of opisthorchiid trematodes of the species Opisthorchis felineus (Rivolta, 1884) and Metorchis bilis (Braun, 1790) using polymerase chain reaction. Parasitol Res 2003; 90: 409-414. https://doi.org/10.1007/s00436-003-0851-4
  34. Parvathi A, Umesha KR, Kumar S, Sithithaworn P, Karunasagar I, Karunasagar I. Development and evaluation of a polymerase chain reaction (PCR) assay for the detection of Opisthorchis viverrini in fish. Acta Trop 2008; 107: 13-16. https://doi.org/10.1016/j.actatropica.2008.04.001
  35. Romashov BV, Romashov VA, Semenov VA, Filimonova LV. Opisthorchiasis in the Upper Don Basin (Voronezh Oblast): The liver fluke fauna, ecological and biological patterns of circulation and foci of Opisthorchiases. 1st ed. Voronezh, Russia. Voronezh State University. 2005, pp 201 (in Russian).
  36. Filimonova LV. A taxonomic review of two subfamilies (Metorchiinae Luhe, 1909 and Pseudamphistominae Yamaguti, 1958) of the family Opisthorchiidae Faust 1929 of the Russian fauna. Top Probl Parasitol Sci 1998; 42: 244-253.
  37. Sitko J, Bizos J, Sherrard-Smith E, Stanton DW, Komorova P, Heneberg P. Integrative taxonomy of European parasitic flatworms of the genus Metorchis Looss, 1899 (Trematoda: Opisthorchiidae). Parasitol Int 2016; 65: 258-267. https://doi.org/10.1016/j.parint.2016.01.011
  38. Brusentsov II, Katokhin AV, Sakharovskaia ZV, Sazonov AE, Ogorodova LM, Fedorova OS, Kolchanov NA, Mordvinov VA. DNA diagnosis of mixed invasions of Opisthorchis felineus and Metorchis bilis by polymerase chain reaction. Med Parazitol (Mosk) 2010; 2: 10-13 (in Russian).
  39. Kiyan VS, Bulashev AK, Tokpan SS, Baibolin ZhK, Alyoshina A. The study of opisthorchiasis infection in the Akmola region. Bulletin of ENU LN Gumilev. Biology series 2015; 6: 134-142 (in Russian).

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