Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
권호 표지
DOI QR코드

DOI QR Code

Cardamonin Inhibits the Expression of Inducible Nitric Oxide Synthase Induced by TLR2, 4, and 6 Agonists

  • Kim, Ah-Yeon (Department of Biomedical Laboratory Science, College of Medical Sciences, SoonChunHyang University) ;
  • Shim, Hyun-Jin (Department of Biomedical Laboratory Science, College of Medical Sciences, SoonChunHyang University) ;
  • Kim, Su-Yeon (Department of Biomedical Laboratory Science, College of Medical Sciences, SoonChunHyang University) ;
  • Heo, Sung-Hye (Department of Biomedical Laboratory Science, College of Medical Sciences, SoonChunHyang University) ;
  • Youn, Hyung-Sun (Department of Biomedical Laboratory Science, College of Medical Sciences, SoonChunHyang University)
  • Received : 2018.04.16
  • Accepted : 2018.06.15
  • Published : 2018.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Toll-like receptors (TLRs) play an important role for host defense against invading pathogens. The activation of TLRs signaling leads to the activation of $NF-{\kappa}B$ and the expression of pro-inflammatory gene products such as cytokines and inducible nitric oxide synthase (iNOS). To evaluate the therapeutic potential of cardamonin, which is a naturally occurring chalcone from Alpinia species (zingiberaceous plant species), $NF-{\kappa}B$ activation and iNOS expression induced by MALP-2 (TLR2 and TLR6 agonist) or LPS (TLR4 agonist) were examined. Cardamonin inhibited the activation of $NF-{\kappa}B$ induced by MALP-2 or LPS. Cardamonin also suppressed the iNOS expression induced by MALP-2 or LPS. These results suggest that cardamonin has the specific mechanism for anti-inflammatory responses by regulating of TLRs signaling pathway.

Keywords

References

  1. Akira S, Takeda K. Toll-like receptor signalling. Nat Rev Immunol. 2004. 4: 499-511. https://doi.org/10.1038/nri1391
  2. Chow YL, Lee KH, Vidyadaran S, Lajis NH, Akhtar MN, Israf DA, Syahida A. (Cardamonin from Alpinia rafflesiana inhibits inflammatory responses in IFN-gamma/LPS-stimulated BV2 microglia via NF-kappaB signalling pathway. Int Immunopharmacol. 2012. 12: 657-665. https://doi.org/10.1016/j.intimp.2012.01.009
  3. Ferrero-Miliani L, Nielsen OH, Andersen PS, Girardin SE. Chronic inflammation: importance of NOD2 and NALP3 in interleukin-1beta generation. Clin Exp Immunol. 2007. 147: 227-235.
  4. Ghosh S, Karin M. Missing pieces in the NF-kappaB puzzle. Cell. 2002. 109 Suppl: S81-96. https://doi.org/10.1016/S0092-8674(02)00703-1
  5. Goncalves LM, Valente IM, Rodrigues JA. An overview on cardamonin. J Med Food. 2014. 17: 633-640. https://doi.org/10.1089/jmf.2013.0061
  6. Green SJ, Meltzer MS, Hibbs JB Jr, Nacy CA. Activated macrophages destroy intracellular Leishmania major amastigotes by an L-arginine-dependent killing mechanism. J Immunol. 1990. 144: 278-283.
  7. Hanafy KA, Krumenacker JS, Murad F. NO, nitrotyrosine, and cyclic GMP in signal transduction. Med Sci Monit. 2001. 7:801-819.
  8. Jantan I, Raweh SM, Sirat HM, Jamil S, Mohd Yasin YH, Jalil J, Jamal JA. Inhibitory effect of compounds from Zingiberaceae species on human platelet aggregation. Phytomedicine. 2008. 15: 306-309. https://doi.org/10.1016/j.phymed.2007.08.002
  9. Kawai T, Akira S. The role of pattern-recognition receptors in innate immunity: update on Toll-like receptors. Nat Immunol. 2010. 11: 373-384. https://doi.org/10.1038/ni.1863
  10. Lim HJ, Lee HS, Ryu JH. Suppression of inducible nitric oxide synthase and cyclooxygenase-2 expression by tussilagone from Farfarae flos in BV-2 microglial cells. Arch Pharm Res. 2008. 31: 645-652. https://doi.org/10.1007/s12272-001-1207-4
  11. Lin YM, Zhou Y, Flavin MT, Zhou LM, Nie W, Chen FC. Chalcones and flavonoids as anti-tuberculosis agents. Bioorg Med Chem. 2002. 10: 2795-2802. https://doi.org/10.1016/S0968-0896(02)00094-9
  12. Moncada S. Nitric oxide: discovery and impact on clinical medicine. J R Soc Med. 1999. 92: 164-169. https://doi.org/10.1177/014107689909200402
  13. Murakami A, Ohigashi H. Targeting NOX, INOS and COX-2 in inflammatory cells: chemoprevention using food phytochemicals. Int J Cancer. 2007. 121: 2357-2363. https://doi.org/10.1002/ijc.23161
  14. Pahl HL. Activators and target genes of Rel/NF-kappaB transcription factors. Oncogene. 1999. 18: 6853-6866. https://doi.org/10.1038/sj.onc.1203239
  15. Palmer RM, Hickery MS, Charles IG, Moncada S, Bayliss MT. Induction of nitric oxide synthase in human chondrocytes. Biochem Biophys Res Commun. 1993. 193: 398-405. https://doi.org/10.1006/bbrc.1993.1637
  16. Park S, Gwak J, Han SJ, Oh S. Cardamonin suppresses the proliferation of colon cancer cells by promoting beta-catenin degradation. Biol Pharm Bull. 2013. 36: 1040-1044. https://doi.org/10.1248/bpb.b13-00158
  17. Turini ME, DuBois RN. Cyclooxygenase-2: a therapeutic target. Annu Rev Med. 2002. 53: 35-57. https://doi.org/10.1146/annurev.med.53.082901.103952
  18. Vallance P. Nitric oxide: therapeutic opportunities. Fundam Clin Pharmacol. 2003. 17: 1-10. https://doi.org/10.1046/j.1472-8206.2003.00124.x
  19. Youn HS. The Anti-Inflammatory Effects of Phytochemicals by the Modulation of Innate Immunity. J Exp Biomed Sci. 2012. 18: 181-192.
  20. Youn HS, Lee JY, Fitzgerald KA, Young HA, Akira S, Hwang DH. Specific inhibition of MyD88-independent signaling pathways of TLR3 and TLR4 by resveratrol: molecular targets are TBK1 and RIP1 in TRIF complex. J Immunol. 2005. 175: 3339-3346. https://doi.org/10.4049/jimmunol.175.5.3339
  21. Youn HS, Lee JY, Saitoh SI, Miyake K, Hwang DH. Auranofin, as an anti-rheumatic gold compound, suppresses LPS-induced homodimerization of TLR4. Biochem Biophys Res Commun. 2006a. 350: 866-871. https://doi.org/10.1016/j.bbrc.2006.09.097
  22. Youn HS, Lee JY, Saitoh SI, Miyake K, Kang KW, Choi YJ, Hwang DH. Suppression of MyD88- and TRIF-dependent signaling pathways of Toll-like receptor by (-)-epigallocatechin-3-gallate, a polyphenol component of green tea. Biochem Pharmacol. 2006b. 72: 850-859. https://doi.org/10.1016/j.bcp.2006.06.021
  23. Youn HS, Saitoh SI, Miyake K, Hwang DH. Inhibition of homodimerization of Toll-like receptor 4 by curcumin. Biochem Pharmacol. 2006c. 72: 62-69. https://doi.org/10.1016/j.bcp.2006.03.022