Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
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Assessment of the Cardioprotection Offered by Fisetin in H2O2-induced Zebrafish (Danio rerio)-Tg (cmlc2: egfp)

  • Lee, Jeong-Soo (Department of Senior Healthcare, BK21 Plus Program, Graduate School, Eulji University) ;
  • Park, Eun-Seok (Department of Biomedical Laboratory Science, Kyungbok University) ;
  • Kim, In-Sik (Department of Senior Healthcare, BK21 Plus Program, Graduate School, Eulji University)
  • Received : 2017.12.21
  • Accepted : 2018.06.15
  • Published : 2018.06.30
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Abstract

The aim of this study was to evaluate the protective function of fisetin, a natural flavonoid in zebrafish heart for the treatment of myocardial infarction in coronary and ischemic heart disease. For this purpose, we induced oxidative stress zebrafish (Danio rerio)-Tg (cmlc2: egfp) by $H_2O_2$ and then administered fisetin, the protective effect of fisetin was determined by measuring the heart rate following fisetin administration. After testing the toxicity of fisetin, we found that the heartt increased in a concentration-dependent manner, however there was no difference between the heart rates of embryos and adults. The improved heart rate demonstrated the cardioprotective effect of fisetin. The result showed that fisetin, at concentration of 3and $5{\mu}M$, significantly increased heart rate compared with the heart with $H_2O_2$ alone. This indicates that fisetin plays an important role in the prevention of heart damage and treatment of cardiovascular diseases caused by oxidative stress due to ischemia / reperfusion.

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