Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effect of Salicornia herbacea L. Supplementation on Tissue Triglyceride Concentrations and PGC-1α & PPAR-γ Expression of Skeletal Muscle of Rats Fed a High-fat Diet

함초의 보충식이가 고지방식이 흰쥐의 혈청 및 조직의 중성지방 농도와 골격근 내 PGC-1α 및 PPAR-γ 단백질 발현에 미치는 영향

  • Cho, Hahyoung (Department of Sports Science, Graduate school, Sunmoon University) ;
  • Kwon, Daekeun (Institute of Sports Health Science, Sunmoon University) ;
  • Kim, JinWoo (Department of Food Science, Sunmoon University) ;
  • Song, Youngju (Institute of Sports Health Science, Sunmoon University)
  • 조하형 (선문대학교 일반대학원 체육학과) ;
  • 권대근 (선문대학교 스포츠건강과학연구소) ;
  • 김진우 (선문대학교 식품과학과) ;
  • 송영주 (선문대학교 스포츠건강과학연구소)
  • Received : 2018.03.02
  • Accepted : 2018.06.21
  • Published : 2018.07.30
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Abstract

This study examined whether the supplementation of Salicornia herbacea L. (SH), a member of the Chenopodiaceae subfamily, affects tissue specific triglyceride (TG) accumulation and the peroxisome proliferator-activated $receptor-{\gamma}$ $coactivator-1{\alpha}$ ($PGC-1{\alpha}$) and peroxisome proliferator-activated $receptor-{\gamma}$ ($PPAR-{\gamma}$) protein expressions of skeletal muscle in rats with a high-fat diet. Sprague-Dawley male rats were randomly divided into three groups: control normal diet group (CD), high-fat diet group (HD), and 5.0% SH supplemented high-fat diet group (SD). The weights of fat tissue of the SD group were reduced by approximately 25%(p<0.01), while the skeletal muscle weight of the SD group increased approximately 5% compared to those in the HD group (p<0.01). The serum and hepatic TG of the SD group decreased approximately 20% compared to those of the HD group (p<0.05). In the protein expression levels in the skeletal muscle, the $PGC-1{\alpha}$ and $PPAR-{\gamma}$ expressions of the SD group were 1.5-folds higher than those of the HD group (p<0.01). From these results, SH supplementation contributes to the improvement of the serum and hepatic TG concentrations, and the $PGC-1{\alpha}$ and $PPAR-{\gamma}$ protein expression levels in the skeletal muscle of fed a high-fat diet. Thus, SH supplementation was effective in reducing fat mass and increasing muscle mass.

본 연구는 함초의 보충식이가 고지방식이 흰쥐의 혈청 및 조직의 중성지방 농도와 골격근 내 $PGC-1{\alpha}$와 $PPAR-{\gamma}$ 단백질 발현에 미치는 영향에 대하여 연구하였다. SD계 수컷 흰쥐를 대조군(CD, n=8), 고지방식이군(HD, n=8), 고지방식이+ 5% 함초 보충식이군(SD, n=8)으로 분류하여 8주간 사육하였다. 그 결과 SD군의 지방조직 중량은 HD군에 비해 약 25%정도 유의하게 감소한 반면 골격근의 중량은 SD군이 HD군에 비해 약 5%정도 유의하게 증가하였다(p<0.01). SD군의 혈청과 간장 내 중성지방은 HD군에 비해 약 20% 유의하게 감소하였다(p<0.05). SD군의 골격근 내 $PGC-1{\alpha}$ 와 $PPAR-{\gamma}$ 단백질 발현은 HD군에 비해 1.5배 유의하게 높게 나타났다(p<0.01). 이상의 결과로 부터 함초 보충은 골격근 내 $PGC-1{\alpha}$와 $PPAR-{\gamma}$ 단백질 발현의 증가를 통하여 지방감소 및 근육량 증가에 효과적이었음이 시사되었다.

Keywords

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