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Toxicity Assessment of Antifouling Agent using the Survival and Population Growth Rate of Marine Rotifer, Brachionus plicatilis

해산로티퍼(Brachionus plicatilis)의 생존 및 개체군 성장률을 이용한 신방오도료(Zinc undecylenate)의 독성평가

  • Hwang, Un-Ki (Marine Ecological Risk Assessment Center, West Sea Fisheries Research Institute, NIFS) ;
  • Choi, Hoon (Marine Ecological Risk Assessment Center, West Sea Fisheries Research Institute, NIFS) ;
  • Park, Yun-Ho (Marine Ecological Risk Assessment Center, West Sea Fisheries Research Institute, NIFS) ;
  • Park, Na-Young (Marine Ecological Risk Assessment Center, West Sea Fisheries Research Institute, NIFS) ;
  • Jang, Soo-Jung (Marine Ecological Risk Assessment Center, West Sea Fisheries Research Institute, NIFS) ;
  • Lee, Seung Min (Marine Ecological Risk Assessment Center, West Sea Fisheries Research Institute, NIFS) ;
  • Choi, Yun-Seok (Marine Ecological Risk Assessment Center, West Sea Fisheries Research Institute, NIFS) ;
  • Yang, Joon-Yong (Marine Ecological Risk Assessment Center, West Sea Fisheries Research Institute, NIFS) ;
  • Lee, Ju-Wook (Marine Ecological Risk Assessment Center, West Sea Fisheries Research Institute, NIFS)
  • 황운기 (국립수산과학원 서해수산연구소 해양생태위해평가센터) ;
  • 최훈 (국립수산과학원 서해수산연구소 해양생태위해평가센터) ;
  • 박윤호 (국립수산과학원 서해수산연구소 해양생태위해평가센터) ;
  • 박나영 (국립수산과학원 서해수산연구소 해양생태위해평가센터) ;
  • 장수정 (국립수산과학원 서해수산연구소 해양생태위해평가센터) ;
  • 이승민 (국립수산과학원 서해수산연구소 해양생태위해평가센터) ;
  • 최윤석 (국립수산과학원 서해수산연구소 해양생태위해평가센터) ;
  • 양준용 (국립수산과학원 서해수산연구소 해양생태위해평가센터) ;
  • 이주욱 (국립수산과학원 서해수산연구소 해양생태위해평가센터)
  • Received : 2018.08.06
  • Accepted : 2018.09.07
  • Published : 2018.09.30

Abstract

Toxicity assessment of antifouling agent, zinc undecylenate (ZU) has been investigated using the rate of survival and population growth in marine rotifer, Brachionus plicatilis. The survival rate of Brachionus plicatilis was determined after 24 h of exposure to ZU and was not affected up to the maximum level of $100.0mg\;L^{-1}$ of ZU. The population growth rate (r) was determined after 72 h of exposure to ZU. It was observed that r in the controls (absence ZU) was greater than 0.5 but exhibited a sudden decrease with an increase in the concentration of ZU. ZU reduced r in a dose-dependent manner and a significant reduction occurred at a concentration greater than $12.5mg\;L^{-1}$. The 50% effective concentration ($EC_{50}$) value of r during ZU exposure was $26.4mg\;L^{-1}$, No-observed-effect-concentration (NOEC) was $6.3mg\;L^{-1}$ and Lowest-observed-effect-concentration (LOEC) was $12.5mg\;L^{-1}$, respectively. Based on the results, it is apparent that ZU concentration greater than $12.5mg\;L^{-1}$ exhibited a toxic effect on the r of zooplankton, B. plicatilis in natural ecosystems.

해산로티퍼(Brachionus plicatilis)의 생존 및 개체군 성장률(r)을 사용하여 Zinc undecylenate (ZU)에 대한 독성평가를 실시하였다. 24 h 동안 ZU에 노출된 B. plicatilis의 생존율은 실험 최고농도 $100mg\;L^{-1}$에서도 영향이 나타나지 않았으나, ZU에 72 h 노출된 개체군 성장률(r)은 농도 의존적으로 감소하는 경향을 나타내, $12.5mg\;L^{-1}$에서 유의적인 감소를 나타냈고 최고농도 $50.0mg\;L^{-1}$에서 개체군 성장률이 90% 이상 감소되었다. ZU에 노출된 B. plicatilis의 개체군 성장률의 반수영향농도($EC_{50}$)값은 $26.4mg\;L^{-1}$, 무영향농도(NOEC)는 $6.3mg\;L^{-1}$, 최소영향농도(LOEC)는 $12.5mg\;L^{-1}$로 나타났다. 자연생태계 내에서 ZU 물질이 해수 중에서 $12.5mg\;L^{-1}$ 이상을 초과하여 나타낼 때 B. plicatilis와 같은 동물성플랑크톤의 개체군 성장률이 영향을 받을 것으로 판단되며, 이러한 연구결과는 신방오도료물질의 생태안정성 평가를 위한 기준치 설정 및 다른 방오도료물질과의 독성치를 비교할 수 있는 유용한 자료로 활용될 것으로 판단된다.

Keywords

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