Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Daegangwhal-Tang Hot Aqueous Extract on Anti-inflammation and Anti-oxidation in RAW 264.7 Macrophage

  • Lee, Jae Sung (Department of Acupuncture & Moxibustion Medicine, Semyung University Chungju Hospital of Korean Medicine) ;
  • Jo, Na Young (Department of Acupuncture & Moxibustion Medicine, Semyung University Jecheon Hospital of Korean Medicine) ;
  • Roh, Jeong Du (Department of Acupuncture & Moxibustion Medicine, Semyung University Jecheon Hospital of Korean Medicine) ;
  • Lee, Cham Kyul (Department of Acupuncture & Moxibustion Medicine, Semyung University Chungju Hospital of Korean Medicine) ;
  • Lee, Eun Yong (Department of Acupuncture & Moxibustion Medicine, Semyung University Chungju Hospital of Korean Medicine)
  • Received : 2018.05.21
  • Accepted : 2018.08.03
  • Published : 2018.08.30
Download PDF
⟨ Previous Next ⟩

Abstract

Background: The objective of this study was to determine the effects of Daegangwhal-Tang (DGHT) hot aqueous extract on production of inflammatory mediators and antioxidants in RAW 264.7 macrophage. Methods: DGHT was extracted with water, filtered, concentrated and freeze-dried to perform. Cytotoxicity of DGHT extract was performed by MTT assay. Activated macrophages were treated with varying concentrations of DGHT extract (10, 50, 100 and $200{\mu}g/mL$), and nitric oxide (NO) and prostaglandin E2 ($PGE_2$) concentrations were measured to detect anti-oxidative effects. Interleukin-6 (IL-6), interleukin-1 beta ($IL-1{\beta}$) and tumor necrosis factor-alpha($TNF-{\alpha}$) concentrations were also measured to detect inflammatory responses to DGHT Results: Cytotoxicity of DGHT extract at concentrations of 10, 50, 100 and $200{\mu}g/mL$ were not observed. NO production was significantly decreased in the DGHT hot aqueous extract $200{\mu}g/mL$ concentration group. $PGE_2$, IL-6, $IL-1{\beta}$ and $TNF-{\alpha}$ production was significantly decreased in the DGHT hot aqueous extract 100 and $200{\mu}g/mL$ concentration groups. DGHT hot aqueous extract appeared to have DPPH free radical scavenging capability at all of concentrations, but did not exceed 50%. Conclusion: These results suggest that DGHT hot aqueous extract has concentration-dependent anti-inflammatory and anti-oxidative effect.

Keywords

References

  1. Na CI. Uisangbogam. Beijing (China): People's Medical Publishing House; 1983. p.370-371. [in Chinese].
  2. Heo J. Donguibogam. Seoul (Korea): Namsandang; 1974. 533p. [in Korean].
  3. Hwang DY. Bangyakhappyun. Seoul (Korea): Namsandang; 1992. p.125-126. [in Korean].
  4. Gilroy DW, Lawrence T, Perretti M, and Rossi AG. Inflammatory resolution: New opportunities for drug discovery. Nat Rev Drug Discov 2004;3:401-416. https://doi.org/10.1038/nrd1383
  5. Korean Acupuncture &Moxibustion Medicine Society. The acupuncture and moxibustion medicine.Seoul (Korea): Hanmibook; 2016. p.573-580. [in Korean].
  6. The Korean Orthopaedic Association. Orthopedics. Seoul (Korea): Choisinuihaksa; 2013. p.209-293. [in Korean].
  7. Lee HK, Jeong SH, Kim SS. Effect of daiganghwaltang on change of IgG anticollagen antibody in type II collagen-induced arthritis. J Korean Rehab Med 1994;4:87-94. [in Korean].
  8. Seo YJ, Koo ST, Yang YJ, Kim S, Yoo IS, Lim KS. Analgesic effect of Daeganghwal-Tang on a model of CFA-induced arthritis. Korean J Acupunct 2004;21:83-99. [in Korean].
  9. Jeong SH, Kim SJ, Seo IB. Anti-pathogenetic and Curative Effects of Taeganghwal-tang (Daqianghou-tang) on the Collagen-Induced Arthritis in Wistar Rats. J Korean Rehab Med 2004;14:79-101. [in Korean].
  10. An JH, Lee MJ. Effects of Daeganghwal-tang(Daqianghuoshang) on the Carrageenan-Induced Arthritis in Animal Model. J Korean Rehab Med 2006;16:79-96. [in Korean].
  11. Kim MK, Oh MS. Inhibi tory Ef fect s of Gamidaeganghwal - tang(Jiaweidaqianghuo-tang) on Rheumatoid Arthritis Induced by Type II Collagen. J Korean Rehab Med 2009;19: 89-102. [in Korean].
  12. Braunwald E, Fauci A, Kasper D, Hauser S, Longo D. Harrison's principles of internal medicine 16th ed. Seoul (Korea):MIP Publisher; 2006. p.2083-2084, p.2222-2227.
  13. Turini ME, DuBois RN. Cyclooxygenase-2: a therapeutictarget. Annu Rev Med 2002;53:35-57. https://doi.org/10.1146/annurev.med.53.082901.103952
  14. Murakami M, Naraba H, Tanioka T, Semmyo N, Nakatani Y, Kojima F et al. Regulation of prostaglandin $E_{2}$ biosynthesis by inducible membrane-associated prostaglandin $E_{2}$ synthase that acts in concert with cyclooxygenase-2. J Biol Chem 2000;275:32783-32792. https://doi.org/10.1074/jbc.M003505200
  15. Merry P, Winyard P G, Morris C J, Grootveld M, Blake D R. Oxygen free radicals, inflammation and synovitis: the current status. Am Rheum Dis 1989;48:864-867. https://doi.org/10.1136/ard.48.10.864
  16. Yoon KJ, Lee EY. Effects of Hot Aqueous and Ethanol Extract from Lonicera japonica Flos on NO and $PGE_{2}$ in Macrophage. J Acupunct Res2012;29:67- 74. [in Korean].
  17. Han SY, Lee EY. Effect of Paeoniae Radix Rubra Extract on the Production of NO and Prostaglandin E2 in LPS-stimulated RAW264.7 Marcrophages. J Acupunct Res 2011;28:77-84. [in Korean].
  18. Kim MS, RohJD. Effects of Ampelopsis Radix Pharmacopuncture Solution on Nitric Oxide and Prostaglandin E2 Production in Macrophages. J Acupunct Res 2011;27:65-73. [in Korean].
  19. Lee SM, Lim SC, Lee YK, Kim JS. Effects of Angelica Gigantis Pharmacopuncture Extracts on the Elastase Activity and DPPH and NO Scavenging Activities. J Acupunct Res 2011;28:35-42. [in Korean].
  20. Lee KH. Effects of MeretrixPharmacopuncture Extracts on the Elastase Activity and DPPH and NO Scavenging Activities. J Acupunct Res 2011;28:117-123. [in Korean].
  21. Choi YJ, Roh JD. Effects of Gamioncheong-decoction Water-extract on Anti-inflammation, Anti-oxidation and Skin Whitening. J Acupunct Res 2015;32:117-126. [in Korean]. https://doi.org/10.13045/acupunct.2015044
  22. Bae BC. Pyojunimsangbangjehak. Seoul (Korea): Seongbosa; 1995. p.398-399. [in Korean].