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Characterization of Ribose-5-Phosphate Isomerase B from Newly Isolated Strain Ochrobactrum sp. CSL1 Producing ʟ-Rhamnulose from ʟ-Rhamnose

  • Shen, Min (School of Chemistry, Biology, and Material Engineering, Suzhou University of Science and Technology) ;
  • Ju, Xin (School of Chemistry, Biology, and Material Engineering, Suzhou University of Science and Technology) ;
  • Xu, Xinqi (Fujian Key Laboratory of Marine Enzyme Engineering, Fuzhou University) ;
  • Yao, Xuemei (School of Chemistry, Biology, and Material Engineering, Suzhou University of Science and Technology) ;
  • Li, Liangzhi (School of Chemistry, Biology, and Material Engineering, Suzhou University of Science and Technology) ;
  • Chen, Jiajia (School of Chemistry, Biology, and Material Engineering, Suzhou University of Science and Technology) ;
  • Hu, Cuiying (School of Chemistry, Biology, and Material Engineering, Suzhou University of Science and Technology) ;
  • Fu, Jiaolong (School of Chemistry, Biology, and Material Engineering, Suzhou University of Science and Technology) ;
  • Yan, Lishi (School of Chemistry, Biology, and Material Engineering, Suzhou University of Science and Technology)
  • 투고 : 2018.02.14
  • 심사 : 2018.04.13
  • 발행 : 2018.07.28

초록

In this study, we attempted to find new and efficient microbial enzymes for producing rare sugars. A ribose-5-phosphate isomerase B (OsRpiB) was cloned, overexpressed, and preliminarily purified successfully from a newly screened Ochrobactrum sp. CSL1, which could catalyze the isomerization reaction of rare sugars. A study of its substrate specificity showed that the cloned isomerase (OsRpiB) could effectively catalyze the conversion of $\text\tiny{L}$-rhamnose to $\text\tiny{L}$-rhamnulose, which was unconventional for RpiB. The optimal reaction conditions ($50^{\circ}C$, pH 8.0, and 1 mM $Ca^{2+}$) were obtained to maximize the potential of OsRpiB in preparing $\text\tiny{L}$-rhamnulose. The catalytic properties of OsRpiB, including $K_m$, $k_{cat}$, and catalytic efficiency ($k_{cat}/K_m$), were determined as 43.47 mM, $129.4sec^{-1}$, and 2.98 mM/sec. The highest conversion rate of $\text\tiny{L}$-rhamnose under the optimized conditions by OsRpiB could reach 26% after 4.5 h. To the best of our knowledge, this is the first successful attempt of the novel biotransformation of $\text\tiny{L}$-rhamnose to $\text\tiny{L}$-rhamnulose by OsRpiB biocatalysis.

키워드

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피인용 문헌

  1. Exploring Multifunctional Residues of Ribose-5-phosphate Isomerase B from Ochrobactrum sp. CSL1 Enhancing Isomerization of D-Allose vol.68, pp.11, 2018, https://doi.org/10.1021/acs.jafc.9b07855
  2. Phosphate sugar isomerases and their potential for rare sugar bioconversion vol.58, pp.9, 2018, https://doi.org/10.1007/s12275-020-0226-x
  3. Engineering ribose-5-phosphate isomerase B from a central carbon metabolic enzyme to a promising sugar biocatalyst vol.105, pp.2, 2018, https://doi.org/10.1007/s00253-020-11075-z
  4. Enhanced isomerization of rare sugars by ribose-5-phosphate isomerase A from Ochrobactrum sp. CSL1 vol.148, pp.None, 2021, https://doi.org/10.1016/j.enzmictec.2021.109789