Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Effects of ID-CBT5101 in Preventing and Alleviating Osteoarthritis Symptoms in a Monosodium Iodoacetate-Induced Rat Model

  • Sim, Boo-Yong (Traditional and Biomedical Research Center, Daejeon University) ;
  • Choi, Hak-Joo (Traditional and Biomedical Research Center, Daejeon University) ;
  • Kim, Min-Goo (Research Laboratories, Ildong Pharmaceutical Company) ;
  • Jeong, Dong-Gu (Research Laboratories, Ildong Pharmaceutical Company) ;
  • Lee, Don-Gil (Research Laboratories, Ildong Pharmaceutical Company) ;
  • Yoon, Jong-Min (Research Laboratories, Ildong Pharmaceutical Company) ;
  • Kang, Dae-Jung (Research Laboratories, Ildong Pharmaceutical Company) ;
  • Park, Soobong (Research Laboratories, Ildong Pharmaceutical Company) ;
  • Ji, Joong-Gu (Department of Oriental Health Care, Joongbu University) ;
  • Joo, In-Hwan (Department of Pathology, College of Oriental Medicine, Daejeon University) ;
  • Kim, Dong-Hee (Traditional and Biomedical Research Center, Daejeon University)
  • Received : 2018.03.28
  • Accepted : 2018.04.28
  • Published : 2018.07.28
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Abstract

Osteoarthritis is a disease that affects the articular cartilage and osseous tissue, and can be worsened by aging, overweight status, and post-traumatic arthritis. The present study aimed to evaluate the effect of ID-CBT5101 (tyndallized Clostridium butyricum) on bone metabolism and the inflammatory response in a monosodium iodoacetate-induced rat model of osteoarthritis. ID-CBT5101 was administered orally at doses of $10^8$ or $10^{10}CFU/day$ for 2 weeks before direct injection of monosodium iodoacetate ($3mg/50{\mu}l$ of 0.9% saline) into the intra-articular space of the rats' right knees. The rats subsequently received the same doses of oral ID-CBT5101 for another 4 weeks. We evaluated the treatment effects based on serum biomarkers, mRNA expression, morphological and histopathological analyses of the knee joints, and weight-bearing distribution analysis. Compared with those in control rats, the ID-CBT5101 treatments significantly reduced the serum concentration of inflammation and bone metabolism markers (i.e., COX-2, IL-6, $LTB_4$, and COMP), and significantly increased the concentration of $IFN-{\gamma}$ and glycosaminoglycans. In addition, the ID-CBT5101 treatments inhibited the mRNA expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases (i.e., MMP-2, MMP-3, MMP-9, MMP-13, TIMP-1, and TIMP-2). Furthermore, the ID-CBT5101 treatments effectively preserved the knee cartilage and synovial membrane, and significantly decreased the amount of fibrous tissue. Moreover, compared with that of the negative control group, the ID-CBT5101 treatments increased the weight-bearing distribution by ${\geq}20%$. The results indicate that ID-CBT5101 prevented and alleviated osteoarthritis symptoms. Thus, ID-CBT5101 may be a novel therapeutic option for the management of osteoarthritis.

Keywords

References

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