Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Inhibition of adhesion and biofilm formation in Escherichia coli O157:H7 by diosmin

다이오스민(diosmin)에 의한 병원성 대장균 세포부착 및 생물막 형성 억제

  • Kim, Hyun Jung (Research Group of Consumer Safety, Korea Food Research Institute) ;
  • Kim, Seung Min (Department of Human Ecology, Korea National Open University)
  • 김현정 (한국식품연구원 소비안전연구단) ;
  • 김승민 (한국방송통신대학교 생활과학과)
  • Received : 2018.07.18
  • Accepted : 2018.08.13
  • Published : 2018.08.31
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Abstract

Escherichia coli O157:H7 is one of the most common foodborne pathogens responsible for outbreaks of hemorrhagic colitis, which can lead to the life-threatening hemolytic-uremic syndrome. In this study, we identified phytochemicals that specifically inhibit the expression of LEE operon in E. coli O157:H7. Among phytochemicals, diosmin decreased the adherence of E. coli O157:H7 towards Caco-2 cells in vitro (p<0.01) and its biofilm formation activity (p<0.05). Quantitative RT-PCR analysis revealed that the transcripts of Ler-regulated genes and genes related to curli production were significantly reduced in the presence of diosmin. However, diosmin does not affect bacterial viability, indicating that the resistance rate to diosmin was remarkably low. Overall, these results provide significant insights into the development of a novel anti-infective agent that is different from conventional antibiotics.

병원성 대장균 O157:H7은 식중독 사고를 일으키는 주된 원인균으로서 항생제 내성 문제를 극복하기 위해서는 병원성 대장균 O157:H7을 제어하기 위한 새로운 방법이 개발되어야 한다. 따라서 본 연구에서는 식물화학물질을 스크리닝하여 병원성 대장균 O157:H7의 부착에 주된 역할을 하는 LEE 오페론의 발현을 감소시키는 물질을 찾고자 하였다. 스크리닝을 통해 선발된 식물화학물질 중 다이오스민은 사람 결장 상피세포 부착능을 3.62배 감소시킴으로써(p<0.01) 양성대조군으로 사용된 미리세틴과 유사한 정도의 효과를 나타냈다. 생물막 형성에 있어서는 다이오스민 처리 시 표현형이 25.6% 감소하여 유의적 차이가 확인되었고(p<0.05), curli 유전자의 발현 역시 미리세틴 처리 때보다 1.57-2.60배 더 유의미하게 감소하는 것으로 나타나 양성대조군보다 더 좋은 효과를 보였다. 한편 다이오스민은 병원성 대장균 O157:H7의 생장에는 아무런 영향을 미치지 않는 것으로 나타나 내성 발생률이 저감화된 새로운 항미생물제재로서 사용 가능하리라 판단된다.

Keywords

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