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Characterization of Cellulase from Bacillus subtilis NSC Isolated from Soil

토양으로부터 단리한 Bacillus subtilis NSC 유래 Cellulase의 특성 규명

  • Kim, Sang Jin (Department of Food Science and Technology, Daegu Catholic University) ;
  • Park, Chang-Su (Department of Food Science and Technology, Daegu Catholic University)
  • 김상진 (대구가톨릭대학교 식품공학전공) ;
  • 박창수 (대구가톨릭대학교 식품공학전공)
  • Received : 2018.11.21
  • Accepted : 2018.11.27
  • Published : 2018.12.30

Abstract

We isolated microorganisms from soil, which is sampled at forest, Kyeonbuk, Korea, as cellulolytic microorganisms. The isolated strains were identified by analysis of 16S rRNA gene from the starins. The result, four kinds of Bacillus subtilis, one kind of Bacillus amyloliquefaciens, and one kind of Bacillus cereus were identified. Among these strains, Bacillus subtilis was selected due to its high cellulase activity and this strain was named as Bacillus subtilis CNS. The optimum pH and temperature of the cellulase from Bacillus subtilis CNS was pH 5.0 and $40^{\circ}C$, respectively. In the investigation of pH and temperature stability, the cellulase from Bacillus subtilis NSC stabled pH 4.0~6.0 range and until $40^{\circ}C$ for 30 min perfectly. In the enzyme activity for various cellulosic substrate, cellulase from Bacillus subtilis CNS showed the highest activity for CM-cellulose. And, the enzyme activities for alkali swollen cellulose, Alpha-cellulose, Sigmacell-cellulose, and Avicel were approximately 31%, 8%, 8% and 4% of activity for CM-cellulose, respectively. In the degradation of CM-cellulose, the 0.26 U/ml and 0.52 U/ml of cellulase showed 0.43 and 0.76 U/ml activity for CM-cellulose after the reaction of 120 min, respectively.

Acknowledgement

Supported by : 대구가톨릭대학교

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