Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
권호 표지
DOI QR코드

DOI QR Code

Genetic Diversity of Echinococcus granulosus Genotype G1 in Xinjiang, Northwest of China

  • Yan, Bin (School of Medicine, Shihezi University) ;
  • Liu, Xiafei (School of Medicine, Shihezi University) ;
  • Wu, Junyuan (College of Animal Science, Tarim University) ;
  • Zhao, Shanshan (School of Medicine, Shihezi University) ;
  • Yuan, Wumei (School of Medicine, Shihezi University) ;
  • Wang, Baoju (Department of Infectious Diseases, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology) ;
  • Wureli, Hazi (School of Animal Science and Technology, Shihezi University) ;
  • Tu, Changchun (Institute of Veterinary Sciences, Academy of Military Medical Sciences) ;
  • Chen, Chuangfu (School of Animal Science and Technology, Shihezi University) ;
  • wang, Yuanzhi (School of Medicine, Shihezi University)
  • Received : 2018.07.31
  • Accepted : 2018.08.18
  • Published : 2018.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

Cystic echinococcosis (CE) caused by E. granulosus is a serious helminthic zoonosis in humans, livestock and wildlife. Xinjiang is one of high endemic province for CE in China. A total of 55 sheep and cattle livers containing echinococcal cysts were collected from slaughterhouses in Changji and Yining City, northern region of Xinjiang. PCR was employed for cloning 2 gene fragments, 12S rRNA and CO1 for analysis of phylogenetic diversity of E. granulosus. The results showed that all the samples collected were identified as G1 genotype of E. granulosus. Interestingly, YL5 and CJ75 strains were the older branches compared to those strains from France, Argentina, Australia. CO1 gene fragment showed 20 new genotype haploids and 5 new genotype haplogroups (H1-H5) by the analysis of Network 5.0 software, and the YLY17 strain was identified as the most ancestral haplotype. The major haplotypes, such as CJ75 and YL5 strains, showed identical to the isolates from Middle East. The international and domestic trade of livestock might contribute to the dispersal of different haplotypes for E. granulosus evolution.

Keywords

References

  1. Eckert J, Gemmell MA, Meslin FX, Meslin, Pawlowski ZS. WHO/OIE manual on echinococcosis in humans and animals: a public health problem of global concern. Paris, France. World Organisation for Animal Health. 2002, pp 1717-1718.
  2. Omer RA, Dinkel A, Romig T, Mackenstedt U, Elnahas AA, Aradaib IE, Ahmed ME, Elmalik KH, Adam A. A molecular survey of cystic echinococcosis in Sudan. Vet Parasitol 2010 11; 169: 340-346. https://doi.org/10.1016/j.vetpar.2010.01.004
  3. Boubaker G, Macchiaroli N, Prada L, Cucher MA, Rosenzvit MC, Ziadinov I, Deplazes P, Saarma U, Babba H, Gottstein B, Spiliotis M. A Multiplex PCR for the Simultaneous Detection and Genotyping of the, Echinococcus granulosus, Complex. PLoS Negl Trop Dis 2013; 7: e2017. https://doi.org/10.1371/journal.pntd.0002017
  4. Obwaller A, Schneider R, Walochnik J, Gollackner B, Deutz A, Janitschke K, Aspock H, Auer H. Echinococcus granulosus strain differentiation based on sequence heterogeneity in mitochondrial genes of cytochrome c oxidase-1 and NADH dehydrogenase-1. Parasitology 2004; 128: 569-575. https://doi.org/10.1017/S0031182004004871
  5. Bowles J, Blair D, Mcmanus DP. Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Mol Biochem Parasitol 1992; 54: 165-173. https://doi.org/10.1016/0166-6851(92)90109-W
  6. Bart JM, Abdukader M, Zhang YL, Lin RY, Wang YH, Nakao M, Ito A, Craig PS, Piarroux R, Vuitton DA, Wen H. Genotyping of human cystic echinococcosis in Xinjiang, PR China. Parasitology 2006; 133: 571-579. https://doi.org/10.1017/S0031182006000734
  7. Zhao CY, Shi FZ, Sheng Y, Li J, Zhao ZM, Han M, Yimamu Y. Regional differentiation characteristics of precipitation changing with altitude in Xinjiang region in recent 50 years. J Glaciol Geocryol 2011; 33: 1203-1213.
  8. People U. Ili Kazakh Autonomous Prefecture. Area. 2015; 11-27.
  9. Zhang Y, Bart JM, Giraudoux P, Craig P, Vuitton D, Wen H. Morphological and molecular characteristics of Echinococcus multilocularis and Echinococcus granulosus mixed infection in a dog from Xinjiang, China. Vet Parasitol 2006; 30;139: 244-248. https://doi.org/10.1016/j.vetpar.2006.03.003
  10. Yu JF, Tan W, Li B, Liu XS, Chang JH, Xiaoye Yang, Rui Wang, Lianru Yang, Xiuxia Li. Gene sequence analysis on CO1 and ND1 of Echinococcus granulosus. Chin J Zoonoses 2014; 30: 812-815 (in Chinese).
  11. Ito A, Nakaya K, Qiu J, Nakao M, Zhen R, Xiao N, Chen X, Giraudoux P, Craig PS. Species identification of human echinococcosis using histopathology and genotyping in northwestern China. Trans R Soc Trop Med Hyg 2008; 102: 585-590. https://doi.org/10.1016/j.trstmh.2008.02.019
  12. Yang J, Jia W, Jing T, Tian G, Cheng X. Analysis of genetic variation of Echinococcus granulosus collected from three provinces in China. Chin J Vet Sci Tech 2004; 34: 12-16.
  13. Zhao SS, Li HY, Yin XP, Liu ZQ, Chen CF, Wang YZ. First detection of Candidatus Rickettsia barbariae, in the flea Vermipsylla alakurt, from north-western China. Parasit Vectors 2016; 9: 325. https://doi.org/10.1186/s13071-016-1614-2
  14. Laurimae T, Kinkar L, Andresiuk V, Haag KL, Ponce-Gordo F, Acosta-Jamett G, Garate T, Gonzalez LM, Saarma U. Genetic diversity and phylogeography of highly zoonotic Echinococcus granulosus genotype G1 in the Americas (Argentina, Brazil, Chile and Mexico) based on 8279 bp of mtDNA. Infect Genet Evol 2016; 45: 290-296. https://doi.org/10.1016/j.meegid.2016.09.015
  15. Kamenetzky L, Gutierrez AM, Canova SG, Haag KL, Guarnera EA, Parra A, Garcia GE, Rosenzvit MC. Several strains of Echinococcus granulosus infect livestock and humans in Argentina. Infect Genet Evol 2002; 2: 129-136. https://doi.org/10.1016/S1567-1348(02)00131-4
  16. Badaraco JL, Ayala FJ, Bart JM, Gottstein B, Haag KL. Using mitochondrial and nuclear markers to evaluate the degree of genetic cohesion among Echinococcus populations. Exp Parasitol 2008; 119: 453-459. https://doi.org/10.1016/j.exppara.2008.02.004
  17. Nakao M, McManus DP, Schantz PM, Craig PS, Ito A. A molecular phylogeny of the genus Echinococcus inferred from complete mitochondrial genomes. Parasitology 2007; 134: 713-722. https://doi.org/10.1017/S0031182006001934
  18. Gao P, Huang L, Guo T. A Study of Difficulties in Developing Herbivorous Livestock in Xinjiang. Finance & Economics of Xinjiang 2015.
  19. Craig P. Epidemiology of echinococcosis in China. Southeast Asian J Trop Med Public Health 2004; 35: 158-169.
  20. Mcmanus DP, Ding Z, Bowles J. A molecular genetic survey indicates the presence of a single, homogeneous strain of Echinococcus granulosus in north-western China. Acta Trop 1994; 56: 7-14. https://doi.org/10.1016/0001-706X(94)90035-3
  21. Anderson TJ, Blouin MS, Beech RN. Population biology of parasitic nematodes: applications of genetic markers. Adv Parasitol 1998; 41: 219-283.
  22. Pour AA, Hosseini SH, Shayan P. Comparative genotyping of Echinococcus granulosus infecting buffalo in Iran using cox1 gene. Parasitol Res 2011; 108: 1229-1234. https://doi.org/10.1007/s00436-010-2170-x
  23. Ma J, Wang H, Lin G, Craig PS, Ito A, Cai Z, Zhang T, Han X, Ma X, Zhang J, Liu Y, Zhao Y, Wang Y. Molecular identification of Echinococcus species from eastern and southern Qinghai, China, based on the mitochondrial cox1 gene. Parasitol Res 2012; 111: 179-184. https://doi.org/10.1007/s00436-012-2815-z
  24. Yanagida T, Mohammadzadeh T, Kamhawi S, Nakao M, Sadjjadi SM, Hijjawi N, Abdel-Hafez SK, Sako Y, Okamoto M, Ito A. Genetic polymorphisms of Echinococcus granulosus sensu stricto in the Middle East. Parasitol Int 2012; 61: 599-603. https://doi.org/10.1016/j.parint.2012.05.014
  25. Wang N, Gu XB, Wang T, Yang GY. Genetic Variability of Echinococcus granulosus Determined by the Mitochondrial Cytochrome c Oxidase Subunit 1 Gene in the Tibet Plateau of China. Chin J Anim Vet Sci 2015; 46: 453-460.
  26. Cai X, Mipam TD. Median-Joining Network Analysis of Phylogeny of Goat Breeds from South China. 2011 5th International Conference on Bioinformatics and Biomedical Engineering. Wuhan, China. IEEE. 2011, pp 1-4.
  27. Kong S, Sanchez-Pacheco SJ, Murphy RW. On the use of median-joining networks in evolutionary biology. Cladistics 2016; 32: 691-699. https://doi.org/10.1111/cla.12147
  28. Hoehe MR. Haplotypes and the systematic analysis of genetic variation in genes and genomes. Pharmacogenomics 2003; 4: 547-570. https://doi.org/10.2217/14622416.4.5.547
  29. Kinkar L Laurimae T, Simsek S, Balkaya I, Casulli A, Manfredi MT, Ponce-Gordo F, Varcasia A, Lavikainen A, Gonzalez LM, Rehbein S, VAN DER Giessen J, Sprong H, Saarma U. High-resolution phylogeography of zoonotic tapeworm Echinococcus granulosus sensu stricto genotype G1 with an emphasis on its distribution in Turkey, Italy and Spain. Parasitology 2016; 143: 1790-1801. https://doi.org/10.1017/S0031182016001530
  30. Daniel MK, Ponce-Gordo F, Cuesta-Bandera C. Genetic identification and host range of the Spanish strains of Echinococcus granulosus. Acta Trop 2004; 91: 87-93. https://doi.org/10.1016/j.actatropica.2004.04.001
  31. Liang R, Chen X, Sun H, Shen Z, Liu J, Wang J, Sun S, Dun W. The feasibility analysis of ovine mtDNA CO1 gene as DNA barcoding in breed identification and phylogenetic. J Yangzhou Univ 2017; 38: 27-32.
  32. Nakao M, Li T, Han X, Ma X, Xiao N, Qiu J, Wang H, Yanagida T, Mamuti W, Wen H, Moro PL, Giraudoux P, Craig PS, Ito A. Genetic polymorphisms of Echinococcus tapeworms in China as determined by mitochondrial and nuclear DNA sequences. Int J Parasitol 2010; 40: 379-385. https://doi.org/10.1016/j.ijpara.2009.09.006
  33. Li XJ, Shi BX, Zhao L, Zhang WB, Yan H, Wang GL, Mi XY. The Epidemic and Control Situation of Hydatid Disease in Xinjiang. Grass-Feeding Livestock 2012; 4: 157.

Cited by

  1. Molecular characterization of Echinococcus granulosus in livestock of Al-Madinah (Saudi Arabia) vol.94, pp.None, 2018, https://doi.org/10.1017/s0022149x20000395
  2. miRNAs and lncRNAs in Echinococcus and Echinococcosis vol.21, pp.3, 2020, https://doi.org/10.3390/ijms21030730
  3. Theileria, Hepatozoon and Taenia infection in great gerbils (Rhombomys opimus) in northwestern China vol.15, pp.None, 2018, https://doi.org/10.1016/j.ijppaw.2021.04.002