Growth Characterization and Cytogenetic Analysis of Three FA Interspecific Hybrid Lilies Bred from Korea

국내 육성 FA종간잡종 나리 3품종의 생육특성 및 세포유전학적 분석

  • Hwang, Yoon-Jung (Departmet of Chemistry & Life Science, Sahmyook University) ;
  • Yan, Wang (Department of Convergence Science, Sahmyook University) ;
  • Kim, Won Hee (Floriculture Research Division, National Institute of Horticultural & Herbal Science) ;
  • Lim, Ki-Byung (Deparment of Horticultural Science, Kyungpook National University) ;
  • Kang, Yun-Im (Floriculture Research Division, National Institute of Horticultural & Herbal Science)
  • 황윤정 (삼육대학교 화학생명과학과) ;
  • ;
  • 김원희 (국립원예특작과학원 화훼과) ;
  • 임기병 (경북대학교 원예과학과) ;
  • 강윤임 (국립원예특작과학원 화훼과)
  • Received : 2018.11.08
  • Accepted : 2018.12.19
  • Published : 2018.12.31


The plant morphological and chromosome characteristics of 'Bonanza', 'Coral Candy' and 'Purple Crystal', a formolongi-Asiatic (FA) interspecific hybrid species bred at the National Institute of Horticultural Science, Rural Development Administration (RDA), were investigated in this study. The flowering time of these species were found to have some variation. 'Bonanza' flowers in the middle to late June (medium-late maturing cultivar), 'Coral Candy' in the mid of June (medium maturing cultivar), and 'Purple Crystal' was observed to be in early June (early maturing cultivar). The flowering direction of all three cultivars are upward facing flowers and having a weak fragrance. The height of the plants was recorded in the range between 101.0 cm ('Purple Crystal') to 142.3 cm ('Bonanza'), thus they are able to develop cut flowers with excellent stem elongation. Flower diameters of 'Bonanza' (17.1 cm) and 'Coral Candy' (16.9 cm) were classified to be large sized flowers. On the other hand, 'Purple Crystal' had a narrow flower diameter (12.3 cm) with an outer petal width of more than 4.0 cm. Leaf length was observed for 'Bonanza' (15.7 cm), 'Coral Candy' (19.7 cm), and 'Purple Crystal' (11.1 cm). Chromosome analysis was done using FISH technique. Results revealed that all three cultivars were observed as triploids (2n=3x=36). FISH analysis also showed 5S/45S rDNA of 'Bonanza', 'Coral Candy' and 'Purple Crystal' as 4/11 loci, 4/12 loci, and 4/11 loci, respectively. The results of the FISH analysis are useful as markers to distinguish cultivars, since the patterns of rDNA observed on the remaining chromosomes are significantly different except FISH patterns of chromosome #3.


Supported by : 농촌진흥청


  1. Arens P, Shahin A, van Tuyl JM (2014) (Molecular) Breeding of Lilium. Acta Hort 1027:113-127
  2. Asano Y (1980) Studies on crosses between distantly related species of lilies. 5th vol. Characteristics of newly obtained hybrids through embryo culture. J Jpn Soc Hort Sci 49:241-250
  3. Chi HS (2000) Interspecific crosses of lily by in vitro pollinated ovules. Bot Bull Acad Sin 41:143-149
  4. Hwang YJ, Song CM, Kwon MK, Kim ST, Kim WH, Han YY, Han TH, Lim KB (2010) An increment of crossing efficiency with consideration of pollen viability analysis in rose. Flower Res J 18:193-200
  5. Kang YI, Joung HY, Goo DH, Choi YJ, Rhee JH (2015) A high propagative FA interspecific hybrids lily ‘Lime Glow’ with light yellow colored tepals for cut flower. Flower Res J 23:217-222
  6. Kim JY, KIM JH (2017) Color shifting cultivar 'Jeolseigain' of Lilium x formolongi Hort. from interspecific crosses with L. brownii var. colchesteri. Flower Res J 25:86-90
  7. Kim JT, Kim SH (2015) Analysis on the production and consumption of the cut lily bulb in world market. Korean J Agric Sci 42:73-79
  8. Kim YJ (1995) Lilium Spp. Bulb plant culture technology. pp 41-98 RDA, Suwon,
  9. Korea Korea Agricultural Trade Information (2016)
  10. Korea Chromosome Database.
  11. Lee HK (2002) Improvement of breeding efficiency in interspecific hybridization of lilies. PhD-thesis, Dept of Hoticulture, Graduate School, Seoul National University
  12. Lim KB, Barba-Gonzalez R, Zhou S, Ramanna MS, van Tuyl JM (2008) Interspecific hybridization in lily (Lilium): Taxonomic and commercial aspects of using species hybrids in breeding. In: Silva JAT (ed) Floriculture, ornamental and plant biotechnology. 5th vol, Global Science Books, Kagawa, Japan, pp 146-151
  13. Lim KB, Chung JD, Van Kronenburg BCE, Ramanna MS, De Jong JH, Van Tuyl JM (2000) Introgression of Lilium rubellum Baker chrmosomes into L. longiforum Thunb.: a genome painting study of the $F_1$ hybrid, $BC_1$ and $BC_2$ progenies. Chromosome Res 8:119-125
  14. Lim KB, Wennekes J, De Jong JH, Jacobsen E, Van Tuyl JM (2001) Karyotype analysis of Lilium longiflorum and Lilium rubellum by chromosome banding and fluorescence in situ hybridization. Genome 44:911-918
  15. McRae EA (1998) Lilies: A guide for growers and collectors. Timber Press, Portland, OG, USA
  16. Ministry for Food, Agriculture, Forestry and Fisheries (MIFAFF) (2016) Statistics for floricultural industry in 2015. Sejong, Korea, pp 346-347
  17. Rural Development Administration (RDA), National Institute of Horticultural & Herbal Science (2016) Lily cultivation manual for bulb and cut flower production. Wanju, Korea, p 40
  18. Waminal NE, Pellerin RJ, Kim N-S, Jayakodi M, Park JY, Yang T-J, Kim HH (2018) Rapid and efficient FISH using pre-labeled oligomer probes. Sci Rep 9:8224
  19. Park KH, Kwon HM (2010) A study on Japanese consumers’ preference for lilies and export expansion strategies. Korean J Food Marketing Economics 28:25-44
  20. Rhee HK, Cho HR, Kim MS, Park SK, Shin HK, Joung HY, Song JS, Lim JH, Kang YI (2013) A FA interspecific hybrid lily ‘Cream Star’ with ivory colored tepals for cut flower. Flower Res J 21:141-145
  21. Rhee HK, Cho HR, Lim JH, Kim MS, Choi SY (2011) A FA intersectional hybrid lily ‘Green Star’ with unspotted greenish yellow petals. Korean J Hort Sci Technol 29: 74-76
  22. Rhee HK, Cho HR, Lim JH, Kim MS, Park SK, Joung HY, Shin HK (2009) ‘Glory Pink’ a FA intersectional lily with unspotted pink petals. Flower Res J 17:40-43
  23. Rhee HK, Cho HR, Lim JH, Shin HK, Kim YJ, Na YJ (2006c) A new FA interspecific hybrid lily, ‘Salmon Bowl’ with orange colored petals. Korean J Breed Sci 38:53-54
  24. Rhee HK, Cho HR, Lim JH, Kim MS, Park SK (2010) A FA intersectional hybrid ‘Cherry Pink’ with deep pink petals. Flower Res J 18:220-223
  25. Rhee HK, Kim KS (2008) Interspecific hybridization and polyploidization in lily breeding. Acta Hort 766:441-445
  26. Rhee HK, Lim JH, Cho HR, Kim YJ (2006a) A new FA interspecific hybrid lily, ‘Goyo’ with trumpet shaped and light orange petals. Korean J Breed Sci 38:143-144
  27. Rhee HK, Lim JH, Cho HR, Kim YJ (2006b) A new FA interspecific hybrid lily, ‘Eunbi’ with unspotted light orange petals. Korean J Breed Sci 38:215-216
  28. Royal Horticultural Society (RHS) (2001) The royal horticultural society colour chart. Royal Hort Soc, London, UK
  29. Rural Development Administration (2015)
  30. Song CY, Park SC, Lee JS, Kim YA, Kim JH (2004) Correlation and combining ability of plant growth and flowering in $F_1$ Hybrids by Diallel cross in Lilium formolongi and L. formosanum. Korean J Hort Sci Technol 22: 107-113
  31. Van Tuyl JM (1997a) Lily production and breeding in the Netherlands. Lecture in RDA
  32. Van Tuyl JM, Chi HS, van Kronenburg BDM, Meijer B (1997b). Interspecif ic lily hybrids: A promise for the future. In: Lilien-Kipnis H, Halevy AH, Borochov A (Eds) Acta Hort 430 ISHS Proc Int' Symp On Flower Bulbs, pp 539-544
  33. Xuan YH, Kim JH (2014) Breeding of Lilium formolongi ‘Baikryungilho’ for summer season cultivation by bulb planting. Flower Res J 22:205-208
  34. Xuan YH, Kyung HY, Kim JH (2009a) Breeding of Lilium formolonga $F_1$ hybrid ‘Eorayeon 1ho’ for cut flower. J Korean Flower Res Soc 17:324-327
  35. Xuan YH, Kyung HY, Kim JH (2009b) Breeding of Lilium formolonga $F_1$ hybrid ‘Eorayeon 2ho’ for cut flower. J Korean Flower Res Soc 17:328-331