Korean Journal of Ecology and Environment (생태와환경)

The Korean Society of Limnology (한국수생태학회)
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Temporal and Spatial Distribution of Microbial Community and Odor Compounds in the Bukhan River System

북한강 수계 미소생물 군집 및 이취미 물질의 시공간적 분포 특성

  • Byun, Jeong-Hwan (Han River Environment Research Center, National Institute of Environmental Research) ;
  • Yu, Mina (Han River Environment Research Center, National Institute of Environmental Research) ;
  • Lee, Eunjeong (Han River Environment Research Center, National Institute of Environmental Research) ;
  • Yoo, Soon-Ju (Han River Environment Research Center, National Institute of Environmental Research) ;
  • Kim, Baik-Ho (Department of Life Science, Hanyang University) ;
  • Byun, Myeong-Seop (Han River Environment Research Center, National Institute of Environmental Research)
  • 변정환 (국립환경과학원 한강물환경연구소) ;
  • 유미나 (국립환경과학원 한강물환경연구소) ;
  • 이은정 (국립환경과학원 한강물환경연구소) ;
  • 유순주 (국립환경과학원 한강물환경연구소) ;
  • 김백호 (한양대학교 생명과학과) ;
  • 변명섭 (국립환경과학원 한강물환경연구소)
  • Received : 2018.10.29
  • Accepted : 2018.12.12
  • Published : 2018.12.31
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Abstract

Odor compounds (geosmin, 2-MIB) have been causing problems in the Bukhan River system, but the causative organisms have not been clearly identified. To evaluate the relationship between dynamics of microbial community and odor compounds, two times monthly monitoring of water quality and microbial community from the three serial lakes (Lake Uiam, Lake Cheongpyeong and Lake Paldang) in the Bukhan River system were conducted from April to October 2017. The odor compounds were analyzed by HS-SPME analysis method using GC/MS. Bacteria communities were identified at the class level by NGS analysis. Actinobacteria and Betaproteobacteria were dominant taxon in bacteria community of three serial lakes. In the case of phytoplankton communities showed that seasonal changes by Bacillariophyceae and Cryptophyceae in spring, Cyanobacteria in summer, and Bacillariophyceae and Cryptophyceae in autumn. Dominant species was Dolichospermum (=Anabaena), Microcystis and Pseudanabaena in Bukhan River system in all study period. At the same time the odors geosmin and 2-MIB were also detected at high concentration. There is a significant positive correlation between proportion of Actinobaceria and 2-MIB concentration (r=0.491, p<0.01). In addition, proportion of cyanobacteria showed a significant correlation of geosmin (r=0.381, p<0.05) and 2-MIB (r=0.386, p<0.05) concentration. In this study, odor compounds in the Bukhan River system are considered to be a direct relationship between with Actinobacteria and cyanobacteria.

본 연구는 2017년 4월부터 10월까지 북한강 수계 3개의 연속댐(의암호(UA), 청평호(CP), 팔당호(PD))의 미소생물 군집과 이취미 농도와 관계를 알아보기 위해 환경요인, 이 취미 물질, 미소생물 군집 등을 조사하였다. 3개 저수지의 박테리아 군집은 주로 Actinobacteria와 Betaproteobacteria가 우점 분류군으로 나타났으며, 계절성은 나타나지 않았다. 식물플랑크톤 군집은 봄철, 규조류 및 은편모조류 여름철 남조류, 가을철 규조류 및 은편모조류 순서로 우점하는 계절성을 보였으며, 북한강 수계에서 출현한 남조류는 Dolichospermum spp., Microcystis aeruginosa, Pseudanabaena spp. 속이 우점 출현하였다. 북한강 수계에 출현한 미소생물 중 이취미 물질을 발생하는 분류군은 Actinobacteria와 남조 Anabaena, Pseudanabaena 속 등이며, 이취미 물질인 Geosmin과 2-MIB가 높게 나타났을 때, 높은 현존량으로 출현하였다. 미소생물과 이취미 물질의 상관관계는 Actinobacteria의 경우 2-MIB (r=0.491, p<0.01)와 유의한 상관성을 나타냈으며, 남조류의 경우 geosmin (r=0.381, p<0.05), 2-MIB (r=0.386, p<0.05)와 유의한 상관성을 나타냈다. 따라서, 북한강 수계에서 나타나는 이취미 물질은 Actinobacteria 및 남조류의 출현과 직접적인 관계가 있을 것으로 사료되며, 남조류 미출현 시 발생하는 높은 농도의 이취미 물질은 Actinobacteria가 생성하는 것으로 판단된다.

Keywords

Acknowledgement

Grant : 남조류 기원 이취미 발생에 대한 분자생물학적 특성

Supported by : 한강물환경연구소

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