The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Ethanol extract of medicinal herbal mixture accelerates hair growth and melanogenesis in vivo and in vitro

한약재 복합추출물이 모발 성장 및 멜라닌 생성 촉진에 미치는 영향

  • Kim, Ji Yoon (Department of Beauty science of Arts, Dongbang Culture University) ;
  • Kim, Yoo-Jin (Department of Herbal Pharmacology, Daegu Haany University) ;
  • Kim, Moon Ju (Department of Beauty science of Arts, Dongbang Culture University) ;
  • Kim, Mi Ryeo (Department of Herbal Pharmacology, Daegu Haany University)
  • 김지윤 (동방문화대학원대학교 뷰티예술학과) ;
  • 김유진 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 김문주 (동방문화대학원대학교 뷰티예술학과) ;
  • 김미려 (대구한의대학교 한의과대학 본초약리학교실)
  • Received : 2018.06.12
  • Accepted : 2018.09.25
  • Published : 2018.09.30
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Abstract

Objectives : This study was performed to determine the transdermal effects of ethanol extract from medicinal herbal mixture (SHJ) on hair growth in C57BL/6 mice and melanogenesis in melanoma cells. Methods : Mice were divided into 3 experimental groups including vehicle (CON), SHJ extract and 5% minoxidil (MNXD, positive control)-treated group. SHJ was applied topically on the hair-shaved skin of C57BL/6 mice everyday for 15 days. The thickness and density of hair with a folliscope and morphometry of hair follicle with a H&E staining were monitored at last day. Also then, hair growth-associated gene expressions were measured by immunoblot assay. Results : The MNXD or SHJ-treated group promoted on hair growth compared to that of vehicle-treated group (CON). Hair density and thickness of MNXD or SHJ treated-group increased compared to that of vehicle application on the 15 days, respectively. Induction of insulin-like growth factor (IGF)-1 and vascular endothelial growth factor (VEGF) were also accelerated by application of SHJ extract compared to those of CON group. But expression of transforming growth factor (TGF)-${\beta}1$ decreased in SHJ treated-group compared to that of CON group. Furthermore, SHJ extract showed to increase melanin contents in a dose-dependent manner. Tyrosinase activity significantly increased in SHJ-treated group compared with CON group in dose-dependant manner. Conclusions : These results suggest that SHJ can be used as a component of cosmeceuticals for hair care via promoting growth and melanogenesis of hair.

Keywords

References

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