The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Effects of aqueous extract from Taxillus chinensis on monosodium iodoacetate-induced osteoarthritis in rats

Monosodium iodoacetate로 유도된 골관절염 동물모델에서 상기생 물추출물의 효과

  • Kim, Joong Sun (Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Lee, A Yeong (Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Moon, Byeong Cheol (Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Kim, Hyo Seon (Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Kim, Wook Jin (Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Kim, Chul (Future Medicine Division, Korea Institute of Oriental Medicine) ;
  • Choi, Goya (Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Kim, Seung-Hyung (Institute of Traditional Medicine and Bioscience, Daejeon University) ;
  • Chun, Jin Mi (Herbal Medicine Research Division, Korea Institute of Oriental Medicine)
  • 김중선 (한국한의학연구원 한약연구부) ;
  • 이아영 (한국한의학연구원 한약연구부) ;
  • 문병철 (한국한의학연구원 한약연구부) ;
  • 김효선 (한국한의학연구원 한약연구부) ;
  • 김욱진 (한국한의학연구원 한약연구부) ;
  • 김철 (한국한의학연구원 미래의학부) ;
  • 최고야 (한국한의학연구원 한약연구부) ;
  • 김승형 (대전대학교 동서생명과학연구원) ;
  • 천진미 (한국한의학연구원 한약연구부)
  • Received : 2018.07.19
  • Accepted : 2018.09.25
  • Published : 2018.09.30
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Abstract

Objectives : The aim of this study was to investigate the protective effects of an aqueous extract from Taxillus chinensis (DC.) Danser (TCE) in Monosodium iodoacetate (MIA)-induced osteoarthritis (OA) rat model. Methods : Sprague Dawley male rats were divided into the following four groups (n=6 per group): Normal (saline control), MIA (MIA-induced OA with vehicle), TCE (MIA-induced with TCE treatment), and IM (MIA-induced with indomethacin treatment). Rats in which OA was induced by MIA were treated with TCE (200 mg/kg) or indomethacin (1 mg/kg) for 4 weeks. Weight-bearing on the hind legs and body weights were measured weekly. At the end of the experiment (3 weeks after MIA injection), serum aspartate aminotransferase and alanine aminotransferase levels were measured to assess the liver toxicity induced by TCE. Its effects on serum inflammatory cytokine levels and tissue histopathology were also evaluated. Results : TCE restored the hind limb weight-bearing distribution. Serum levels of Interleukin 6 (IL-6), Tumor necrosis factor alpha (TNF-${\alpha}$) and Leukotriene B4 (LTB4) were significantly higher in the MIA group than in the Normal group, but serum IL-6 levels were significantly lower in the TCE group. In the TCE group, the synovial membrane was protected in hematoxylin and eosin and Safranin-O staining, respectively. Conclusions : TCE recovered the hind paw weight bearing distribution, inhibited the production of inflammatory cytokine, and protected synovial tissue and cartilage in the OA rat model. Therefore, TCE appears to be an effective therapeutic agent for treating OA and OA-related symptoms.

Keywords

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