DOI QR코드

DOI QR Code

Draft genome sequence of Pseudoalteromonas sp. meg-B1 isolated from marine sediment

해양퇴적물로부터 분리된 Pseudoalteromonas sp. meg-B1의 유전체 분석

  • Park, Soo-Je (Department of Biology, Jeju National University) ;
  • Park, Sewook (Food Microbiology Division, Ministry of Food and Drug Safety)
  • 박수제 (제주대학교 생물학과) ;
  • 박세욱 (식품의약품안전처 식품의약품안전평가원 미생물과)
  • Received : 2018.05.28
  • Accepted : 2018.06.14
  • Published : 2018.09.30

Abstract

Pseudoalteromonas sp. meg-B1 belonging to Gammaproteobacteria was isolated from marine sediment in Jeju island. Here, we report the draft genome sequence of strain meg-B1 with a size of approximately 4.15 Mbp and a mean G + C content of 41.2%. The draft genome included 3,606 coding sequences, and 9 ribosomal RNA and 94 transfer RNA genes. In the draft genome, genes (e.g. choline dehydrogenase) involved in the accumulation of compatible solutes required for survival in marine environments have been identified.

Gammaproteobacteria에 속하는 Pseudoalteromonas sp. meg-B1을 제주도 해양 퇴적물로부터 분리하였다. 본 연구에서는 대략 4.15 Mb의 크기와 41.2%의 평균 G + C 함량을 가진 meg-B1 균주의 완전한 유전체를 보고한다. 유전체는 3,606개의 코딩 서열, 9개의 리보솜 RNA 및 94개의 전사 RNA 유전자가 존재하며, 한 개의 완전한 프로파지 영역이 발견되었다. 본 유전체는 해양환경에서 생존하기 위한 삼투화합성 용질합성과 관련된 유전자(예, choline dehydrogenase)들이 확인되었다.

Keywords

References

  1. Angiuoli SV, Gussman A, Klimke W, Cochrane G, Field D, Garrity G, Kodira CD, Kyrpides N, Madupu R, Markowitz V, et al. 2008. Toward an online repository of standard operating procedures (SOPs) for (meta)genomic annotation. OMICS 12, 137-141. https://doi.org/10.1089/omi.2008.0017
  2. Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M, Kulikov AS, Lesin VM, Nikolenko SI, Pham S, Prjibelski AD, et al. 2012. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J. Comput. Biol. 19, 455-477. https://doi.org/10.1089/cmb.2012.0021
  3. Gauthier G, Gauthier M, and Christen R. 1995. Phylogenetic analysis of the genera Alteromonas, Shewanella, and Moritella using genes coding for small-subunit rRNA sequences and division of the genus Alteromonas into two genera, Alteromonas (emended) and Pseudoalteromonas gen. nov., and proposal of twelve new species combinations. Int. J. Syst. Bacteriol. 45, 755-761. https://doi.org/10.1099/00207713-45-4-755
  4. Grissa I, Vergnaud G, and Pourcel C. 2007. CRISPRFinder: a web tool to identify clustered regularly interspaced short palindromic repeats. Nucleic Acids Res. 35, W52-57. https://doi.org/10.1093/nar/gkm360
  5. Ivanova EP, Ng HJ, and Webb HK. 2014. The family Pseudoalteromonadaceae, pp. 575-582. In Rosenberg E, DeLong EF, Lory S, Stackebrandt E, and Thompson F. (eds.), The prokaryotes: Gammaproteobacteria. Springer, Berlin, Heidelberg, Germany.
  6. Parks DH, Imelfort M, Skennerton CT, Hugenholtz P, and Tyson GW. 2015. CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes. Genome Res. 25, 1043-1055. https://doi.org/10.1101/gr.186072.114
  7. Zhou Y, Liang Y, Lynch KH, Dennis JJ, and Wishart DS. 2011. PHAST: a fast phage search tool. Nucleic Acids Res. 39, W347-352. https://doi.org/10.1093/nar/gkr485