Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

The Korea Academia-Industrial cooperation Society (한국산학기술학회)
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Association with Kinetic Characteristics of sperm in Duroc Boar and the Zygote Arrest 1 gene Polymorphism (g.2540T>C)

Zygote arrest 1 유전자 변이(g.2540T>C)와 두록 정액의 운동학적 특성과의 연관성 분석

  • Lee, Mi Jin (National Institute of Animal Science, RDA) ;
  • Ko, Jun Ho (National Institute of Animal Science, RDA) ;
  • Cho, Kyu Ho (National Institute of Animal Science, RDA) ;
  • Choi, Tae Jeong (National Institute of Animal Science, RDA) ;
  • Kim, Yong Min (National Institute of Animal Science, RDA) ;
  • Kim, Young Sin (National Institute of Animal Science, RDA) ;
  • Jin, Dong Il (Department of Animal Science & Biotechnology, Chungnam National University) ;
  • Cho, Eun seok (National Institute of Animal Science, RDA) ;
  • Kim, Nam Hyung (College of Animal Resource Sciences, Chungbuk National University)
  • 이미진 (농촌진흥청 국립축산과학원 양돈과) ;
  • 고준호 (농촌진흥청 국립축산과학원 양돈과) ;
  • 조규호 (농촌진흥청 국립축산과학원 양돈과) ;
  • 최태정 (농촌진흥청 국립축산과학원 양돈과) ;
  • 김용민 (농촌진흥청 국립축산과학원 양돈과) ;
  • 김영신 (농촌진흥청 국립축산과학원 양돈과) ;
  • 진동일 (충남대학교 농업생명과학대학 동물자원과학부) ;
  • 조은석 (농촌진흥청 국립축산과학원 양돈과) ;
  • 김남형 (충북대학교 농업생명환경대학 축산학과)
  • Received : 2018.06.11
  • Accepted : 2018.09.07
  • Published : 2018.09.30
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Abstract

The sperm quality is determined by the kinetic characteristics and acrosome integrity of the sperm. In the previous studies, analysis of semen quality had large errors because those experiments by using microscope had been conducted by people. In recent years, the molecular biological methods have been newly developed to complement the previous techniques. The ZAR1 gene is known to be a gene that affects early embryonic development in vertebrates, but there is no study of the association with semen. In this study, we analyzed the association between the kinetic characteristics and ZAR1 single nucleotide polymorphism (SNP) genotype. To detect the SNPs, we performed sequencing using genomic DNA from the whole bloods of Duroc pigs. We identified an SNP in the ZAR1 gene g.2540T>C. ZAR1 SNP genotypeing in 105 pigs revealed that the major and minor alleles were T and C, respectively. After we analyzed the association between the kinetic characteristics of sperm and the ZAR1 SNP genotype, we found a significant association in MOT (p<0.01), VSL (p<0.05) of the kinetic characteristics in the Duroc boars. It was confirmed that the boars with T allele were lower in MOT and VSL than C allele. Therefore, pigs with C allele are judged to be better at the MOT and VSL of semen. Based on these results, ZAR1 SNP genotyping may be a useful molecular biomarker to improve semen quality by applying molecular breeding technology.

정액의 품질은 정자의 운동학적 특성 및 첨체의 온전성 등에 의해서 결정된다. 이전 연구들에서 정액 품질 검사는 현미경을 이용하여 사람이 직접적으로 수행하기 때문에 오차가 컸다. 최근에는 이러한 기법을 보완하고자 분자생물학적 방법을 통한 검사 방법이 새로이 대두되고 있다. ZAR1 유전자는 척추동물의 초기 배아 발달에 영향을 미치는 유전자로 알려져 있지만 정액과 연관성 연구는 진행되어 있지 않다. 본 연구는 ZAR1 유전자의 SNP을 탐색하고 정액의 운동학적 특성과의 연관성을 규명하였다. SNP을 탐색 및 연관성 분석을 하고자 두록 수퇘지 105두의 혈액으로부터 추출한 DNA로 부터 ZAR1 유전자의 전체 염기서열 분석을 실시하였고, 105두의 정액의 운동학적 특성을 분석하였다. 그 결과, ZAR1의 염색체 2540번째 T 서열이 C로 변환되는 것을 확인하였고, ZAR1 SNP의 유전자형을 분석한 결과 major allel은 T 서열이며 minor allele은 C로 확인되었다. ZAR1의 유전자형과 정액의 운동학적 특성과의 연관성 분석 결과 MOT (Motility) (p<0.01)와 VSL (Straight-line Velocity) (p<0.05)에서 유의적인 차이가 나타났다. 또한, 운동학적 특성과 ZAR1 SNP을 비교하였을 때, T allele을 가진 유전자형이 C allele에 비해 MOT와 VSL을 감소시키는 것으로 확인하였다. 따라서 C allele을 가진 돼지가 정액의 MOT와 VSL에서 더 좋은 것으로 판단된다. 이러한 결과들은 우수한 정액을 생산하는 돼지를 판별하는 유전자 진단 기법의 기초자료로 활용할 수 있을 것으로 사료된다.

Keywords

References

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