Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Production of bioactive ginsenoside Rg3(S) and compound K using recombinant Lactococcus lactis

  • Li, Ling (Zhejiang Provincial Key Laboratory for Chemistry and Biology Processing Technology of Farm Produces, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology) ;
  • Lee, Soo Jin (Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural, and Food Sciences, Chungbuk National University) ;
  • Yuan, Qiu Ping (Zhejiang Provincial Key Laboratory for Chemistry and Biology Processing Technology of Farm Produces, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology) ;
  • Im, Wan Taek (Department of Biotechnology, Hankyong National University) ;
  • Kim, Sun Chang (Department of Biological Science, KAIST) ;
  • Han, Nam Soo (Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural, and Food Sciences, Chungbuk National University)
  • Received : 2016.12.27
  • Accepted : 2017.04.17
  • Published : 2018.10.15
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Abstract

Background: Ginsenoside Rg3(S) and compound K (C-K) are pharmacologically active components of ginseng that promote human health and improve quality of life. The aim of this study was to produce Rg3(S) and C-K from ginseng extract using recombinant Lactococcus lactis. Methods: L. lactis subsp. cremoris NZ9000 (L. lactis NZ9000), which harbors ${\beta}$-glucosidase genes (BglPm and BglBX10) from Paenibacillus mucilaginosus and Flavobacterium johnsoniae, respectively, was reacted with ginseng extract (protopanaxadiol-type ginsenoside mixture). Results: Crude enzyme activity of BglBX10 values comprised 0.001 unit/mL and 0.003 unit/mL in uninduced and induced preparations, respectively. When whole cells of L. lactis harboring pNZBglBX10 were treated with ginseng extract, after permeabilization of cells by xylene, Rb1 and Rd were converted into Rg3(S) with a conversion yield of 61%. C-K was also produced by sequential reactions of the permeabilized cells harboring each pNZBgl and pNZBglBX10, resulting in a 70% maximum conversion yield. Conclusion: This study demonstrates that the lactic acid bacteria having specific ${\beta}$-glucosidase activity can be used to enhance the health benefits of Panax ginseng in either fermented foods or bioconversion processes.

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References

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