Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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Antioxidant effects and tyrosinase and elastase inhibitory activities of mountain ginseng adventitious roots extracts at different ethanol concentrations

  • Ko, Hyun Min (Major in Plant Resource and Environment, SARI, Jeju National University) ;
  • Eom, Tae Kil (Subtropical/Tropical Organism Gene Bank, SARI, Jeju National University) ;
  • Kim, Kyeoung Cheol (Major in Plant Resource and Environment, SARI, Jeju National University) ;
  • Kim, Chul Joong (Hwajinbiocosmetics) ;
  • Lee, Jae Geun (Hwajinbiocosmetics) ;
  • Kim, Ju-Sung (Major in Plant Resource and Environment, SARI, Jeju National University)
  • Received : 2018.01.15
  • Accepted : 2018.05.23
  • Published : 2018.09.30
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Abstract

To use mountain ginseng adventitious roots (MGAR), which is superior in functionality compared to ginseng, and to mass produce raw materials as a functional cosmetic material, the antioxidant and cosmeceutical activities of the extracts of MGAR at different ethanol concentrations (0, 20, 40, 60 and 80%) were compared and analyzed. At first, different ethanol concentrations were compared to determine the best solvent for the extraction of phenolic compounds from MGAR. An 80% ethanol extract with 10.07 mg of gallic acid equivalents/g sample and 0.94 mg of quercetin equivalents/g sample was the best solvent in the extraction of phenolic compounds. However, the 60% ethanol extract had the highest antioxidant activity, evident by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, Trolox equivalent antioxidant capacity (35.01 mM Trolox/g sample) and ferric reducing antioxidant power (460.94 mM $FeSO_4/g$ sample). However, the inhibitory activities of the 80% ethanol extract from the MGAR against tyrosinase and elastase were higher than those of the other extracts. The results of this study show that the extract of MGAR can be used as an easily accessible source of natural antioxidant and as a possible cosmetic ingredient in the cosmeceutical industry. However, the components responsible for the antioxidant and cosmeceutical activities of the MGAR extracts are currently unclear. Therefore, it is suggested that further studies should be carried out to isolate and identify the antioxidant and cosmeceutical components in MGAR.

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References

  1. Ahn YM, Park HS, Kwon KR. 2007. Anti-cancer anti-oxidant efficacies of wild ginseng and cultivated wild ginseng of Korea and China. Korean Journal of pharmacopuncture 10:5-16. [in Korean].
  2. Benzie IFF, Strain JJ. 1996. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": The FRAP assay. Analytical Biochemistry 39:70-76.
  3. Blois MS. 1958. Antioxidant determinations by use of a stable free radical. Nature 181:1199-1200. https://doi.org/10.1038/1811199a0
  4. Chang TS. 2009. An updated review of tyrosinase inhibitors. International Journal of Molecular Science 10:2440-2475. https://doi.org/10.3390/ijms10062440
  5. Cho S, Lee J, Kim YM, Jung YS, Kim HB, Cho EJ, Lee S. 2017. Chemical composition of different parts of ramie (Boehmeria nivea). Korean Journal of Agricultural Science 44:95-103. [in Korean]
  6. Choi WY, Lee CG, Seo YC, Song CH, Lim HW, Lee HY. 2012. Effect of high pressure and steaming extraction processes on ginsenosides Rg3 and Rh2 contents of cultured-root in wild ginseng (Panax ginseng C. A. Meyer). Korean Journal of Medicinal Crop Science 20:270-276. [in Korean] https://doi.org/10.7783/KJMCS.2012.20.4.270
  7. Choi YE, Jeong JH. 2003. Recent progress of ginseng biotechnology and progress toward food application. Food Industry and Nutrition 8:24-29. [in Korean]
  8. Gilchrest BA, Park HY, Eller MS, Yaar M. 1996. Mechanisms of ultraviolet light induced pigmentation. Photochemistry and Photobiology 63:1-10. https://doi.org/10.1111/j.1751-1097.1996.tb02988.x
  9. Heim KE, Tagliaferro AR, Bobilya DJ. 2002. Flavonoid antioxidant: Chemistry, metabolism and structure activity relationships. The Journal of Nutritional Biochemistry 13:572-584. https://doi.org/10.1016/S0955-2863(02)00208-5
  10. Ichihashi M, Ueda M, Budiyanto A, Bito T, Oka M, Fukunaga M, Tsuru K, Horikawa T. 2003. UV-induced skin damage. Toxicology 189:21-39. https://doi.org/10.1016/S0300-483X(03)00150-1
  11. Imokawa G. 2009. Mechanism of UVB-induced wrinkling of skin: Paracrine cytokine linkage between keratinocytes and fibroblasts leading to the stimulation of elastase. Journal of Investigative Dermatology Symposium Proceedings 14:36-43. https://doi.org/10.1038/jidsymp.2009.11
  12. Kang KM, Lee JY, Kim MU, Lee SH. 2016. Effects of quality characteristics and antioxidant activity of Korean cultivated wild ginseng extract. Journal of Korean Society Food Science and Nutrition 45:1740-1746. [in Korean] https://doi.org/10.3746/jkfn.2016.45.12.1740
  13. Kim CJ, Seong ES, Yoo JH, Lee JG, Kim NJ, Choi SK, Lim JD, Yu CY. 2016. Biological activity of Panax ginseng CA Meyer culture roots fermented with microorganisms. Korean Journal of Medicinal Crop Science 24:191-197. [in Korean] https://doi.org/10.7783/KJMCS.2016.24.3.191
  14. Kim EJ, Choi JY, Yu M, Kim MY, Lee S, Lee BH. 2012. Total polyphenols, total flavonoid contents, and antioxidant activity of Korean natural and medicinal plants. Korean Journal of Food Science and Technology 44:337-342. [in Korean] https://doi.org/10.9721/KJFST.2012.44.3.337
  15. Kim JW, Lee SH, No HK, Hong JH, Youn KS. 2010. Antioxidant properties of cultured wild ginseng root extracts. The Korean Society of Food Preservation 17:861-866. [in Korean]
  16. Lee BB, Park SR, Han CS, Han DY, Park EJ, Park HR, Lee SC. 2008. Antioxidant activity and inhibition activity against ${\alpha}$-amylase and ${\alpha}$-glucosidase of Viola mandshurica extracts. Journal of the Korean Society of Food Science and Nutrition 37:405-409. https://doi.org/10.3746/jkfn.2008.37.4.405
  17. Lee KK, Kim JH, Cho JJ, Choi JD. 1999. Inhibitory effects of 150 plant extracts on elastase activity, and their anti-inflammatory effects. International Journal of Cosmetic Science 21:71-82. https://doi.org/10.1046/j.1467-2494.1999.181638.x
  18. Lee MS. 2010. Cisplatin and extract of tissue cultured mountain ginseng-induced apoptosis in human cervical cancer cells. Korean Journal of Microscopy 40:133-138. [in Korean]
  19. Lee SE, Kim YS, Kim JE, Bang JK, Seong NS. 2004. Antioxidant activity of Ulmus davidiana var. japonica N. and Hemipteleae davidii P. Korean Journal of Medicinal Crop Science 12:321-327. [in Korean]
  20. Manach C, Scalbert A, Morand C, Remesy C, Jimenez L. 2004. Polyphenols: Food sources and bioavailability. The American Journal of Clinical Nutrition 79:727-747. https://doi.org/10.1093/ajcn/79.5.727
  21. Mizuno M, Yamada J, Terai H, Kozukue N, Lee YS, Tsuchida H. 1994. Differences in immunomodulating effects between wild and cultured Panax ginseng. Biochemical and Biophysical Research Communications 200:1672-1678. https://doi.org/10.1006/bbrc.1994.1644
  22. Park H, Park AK. 2007. Research on mountain ginseng. Korean Society of Ginseng 12:311-320. [in Korean]
  23. Park SJ, Yoo SM, Kim YE. 2012. Nutritional characteristics and screening of biological activity of cultured wild ginseng roots. Korean Journal of Food and Nutrition 25:729-736. [in Korean] https://doi.org/10.9799/ksfan.2012.25.4.729
  24. Pillai S, Oresajo C, Hayward J. 2005. Ultraviolet radiation and skin aging: Roles of reactive oxygen species, inflammation and protease activation, and strategies for prevention of inflammation induced matrix degradation - a review. International Journal of Cosmetic Science 27:17-34. https://doi.org/10.1111/j.1467-2494.2004.00241.x
  25. Saha AK, Rahman MR, Shahriar M, Saha SK, Azad NA, Das S. 2013. Screening of six ayuvedic medicinal plant extracts for antioxidant and cytotoxic activity. Journal of Pharmacognosy and Phytochemistry 2:181-188.
  26. Shin MH. 2001. Study of mountain ginseng adventitious culture and application. Journal of the Society of Cosmetic Scientists of Korea 27:45-56. [in Korean]
  27. Siedle B, Hrenn A, Merfort I. 2007. Natural compounds as inhibitors of human neutrophil elastase. Planta Medica 73:401-420. https://doi.org/10.1055/s-2007-967183
  28. Yamaguchi Y, Brenner M, Hearing VJ. 2007. The regulation of skin pigmentation. Journal of Biological Chemistry 282:27557-27561. https://doi.org/10.1074/jbc.R700026200
  29. Yang SJ, Choe TB. 2011. Antioxidant activity and whitening effect of Forsuthiae fructus extracts. Korean Journal of Medicinal Crop Science 19:472-477. [in Korean] https://doi.org/10.7783/KJMCS.2011.19.6.472
  30. Yang WT, Kim KS, Kwon YS, Kim DH, Kim DH. 2016. Whitening and anti-aging of Cistanche deserticola extract. Journal of Plant Biotechnology 43:492-499. [in Korean] https://doi.org/10.5010/JPB.2016.43.4.492
  31. Yoo BS, Chang MS, Byun SY. 2003. Characterization of cell cultures and ginsenoside production by cultured ginseng and wild mountain ginseng. Korean Journal of Biotechnology and Bioengineering 18:133-139. [in Korean]
  32. Yoo YG, Joung MS, Lee YH, Choi JW, Kim JH, Paek KY. 2004. A study on the effect of mountain ginseng adventitious roots extract. Journal of the Society of Cosmetic Scientists of Korea 30:377-383. [in Korean]
  33. Yoon NY, Eom TK, Kim MM, Kim SK. 2009. Inhibitory effect of phlorotannins isolated from Ecklonia cava on mushroom tyrosinase activity and melanin formation in mouse B16F10 melanoma cells. Journal of Agricultural and Food Chemistry 57:4124-4129. https://doi.org/10.1021/jf900006f
  34. Zulueta A, Esteve MJ, Frigola A. 2009. ORAC and TEAC assays comparison to measure the antioxidant capacity of food products. Food Chemistry 114:310-316. https://doi.org/10.1016/j.foodchem.2008.09.033

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