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Alternaria brassicifolii sp. nov. Isolated from Brassica rapa subsp. pekinensis in Korea

  • Deng, Jian Xin (Department of Plant Protection, College of Agriculture, Yangtze University) ;
  • Li, Mei Jia (Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agriculture Sciences) ;
  • Paul, Narayan Chandra (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Oo, May Moe (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Lee, Hyang Burm (Division of Applied Bioscience & Biotechnology, College of Agriculture & Life Sciences, Chonnam National University) ;
  • Oh, Sang-Keun (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Yu, Seung Hun (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
  • 투고 : 2018.01.12
  • 심사 : 2018.03.18
  • 발행 : 2018.06.01

초록

A new species belonging to the genus Alternaria was isolated from the necrotic leaf spots of Brassica rapa subsp. pekinensis in Yuseong district, Daejeon, Korea. It is an occasional isolate, not an etiological agent, which is morphologically similar to A. broccoli-italicae, but differs in conidial size and conidiophore shape. Phylogenetic analysis using the sequence datasets of the internal transcribed spacer (ITS) region of the rDNA, glyceraldehyde-3-phosphate dehydrogenase (gpd), and plasma membrane ATPase genes showed that it is distantly related to A. broccoli-italicae and closely related to Alternaria species in the section Pseudoalternaria, which belonged to a clade basal to the section Infectoriae. Morphologically, the species is unique because it produces solitary conidia or conidial chains (two units), unlike the four members in the section Pseudoalternaria that produce conidia as short branched chains. It exhibits weak pathogenicity in the host plant. This report includes the description and illustration of A. brassicifolii as a new species.

키워드

참고문헌

  1. Yu SH. Alternaria species and allied genera. Incheon: National Institute of Biological Resources Ministry of Environment; 2015.
  2. Nowicki M, Nowakowska M, Niezgoda A, et al. Alternaria black spot of crucifers: symptoms, importance of disease, and perspectives of resistance breeding. Veg Crops Res Bull. 2012;76:5-19.
  3. Kumar D, Maurya N, Bharati YK, et al. Alternaria blight of oilseed Brassicas: a comprehensive review. Afr J Microbiol Res. 2014;8:2816-2829. https://doi.org/10.5897/AJMR2013.6434
  4. Simmons EG. Alternaria: an identification manual. CBS Biodiversity Series 6. Utrecht: Centraalbureau voor Schimmelcultures; 2007.
  5. Andersen B, Kroger E, Roberts RG. Chemical and morphological segregation of Alternaria alternata, A. gaisen and A. longipes. Mycol Res. 2001;105:291-299. https://doi.org/10.1017/S0953756201003446
  6. Zhang TY. Flora fungorum sinicorum, vol. 16 Alternaria. Beijing: Science Press; 2003.
  7. Nishikawa J, Nakashima C. Taxonomic characterization and experimental host ranges of four newly recorded species of Alternaria from Japan. J Phytopathol. 2013;161:604-616. https://doi.org/10.1111/jph.12108
  8. Pryor BM, Bigelow DM. Molecular characterization of Embellisia and Nimbya species and their relationship to Alternaria, Ulocladium and Stemphylium. Mycologia. 2003;95:1141-1154. https://doi.org/10.1080/15572536.2004.11833024
  9. Hong SG, Cramer RA, Lawrence CB, et al. Alt a 1 allergen homologs from Alternaria and related taxa: analysis of phylogenetic content and secondary structure. Fungal Genet Biol. 2005;42:119-129. https://doi.org/10.1016/j.fgb.2004.10.009
  10. Park MS, Romanoski CE, Pryor BM. A re-examination of the phylogenetic relationship between the causal agents of carrot black rot, Alternaria radicina and A. carotiincultae. Mycologia. 2008;100:511-527. https://doi.org/10.3852/07-186R1
  11. Toth B, Csosz M, Szabo-Hever A, et al. Alternaria hungarica sp. nov., a minor foliar pathogen of wheat in Hungary. Mycologia. 2011;103:94-100. https://doi.org/10.3852/09-196
  12. Deng JX, Cho HS, Paul NC, et al. A novel species belonging to Alternaria isolated from Peucedanum japonicum in Korea. Mycobiology. 2014;42:12-16. https://doi.org/10.5941/MYCO.2014.42.1.12
  13. Woudenberg J, Truter M, Groenewald J, et al. Large-spored Alternaria pathogens in section Porri disentangled. Stud Mycol. 2014;79:1-47. https://doi.org/10.1016/j.simyco.2014.07.003
  14. Woudenberg JH, Groenewald JZ, Binder M, et al. Alternaria redefined. Stud Mycol. 2013;75:171-212. https://doi.org/10.3114/sim0015
  15. Lawrence DP, Rotondo F, Gannibal PB. Biodiversity and taxonomy of the pleomorphic genus Alternaria. Mycol Prog. 2016;15:3. https://doi.org/10.1007/s11557-015-1144-x
  16. Park MS, Seo GS, Bae KS, et al. Characterization of Trichoderma spp. associated with green mold of Oyster mushroom by PCR-RFLP and sequence analysis of ITS regions of rDNA. Plant Pathol J. 2005;21:229-236. https://doi.org/10.5423/PPJ.2005.21.3.229
  17. White TJ, Bruns T, Lee S, et al. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, editors. PCR protocols: a guide to methods and applications. San Diego: Academic Press; 1990. p. 315-322.
  18. Berbee ML, Pirseyedi M, Hubbard S. Cochliobolus phylogenetics and the origin of known, highly virulent pathogens, inferred from ITS and glyceraldehyde-3-phosphate dehydrogenase gene sequences. Mycologia. 1999;91:964-977. https://doi.org/10.2307/3761627
  19. Lawrence DP, Gannibal PB, Dugan FM, et al. Characterization of Alternaria isolates from the infectoria species-group and a new taxon from Arrhenatherum, Pseudoalternaria arrhenatheria sp. nov. Mycol Progress. 2014;13:257-276. https://doi.org/10.1007/s11557-013-0910-x
  20. Poursafar A, Ghosta Y, Orina AS, et al. Taxonomic study on Alternaria sections Infectoriae and Pseudoalternaria associated with black (sooty) head mold of wheat and barley in Iran. Mycol Progress. 2018;17:343-356. https://doi.org/10.1007/s11557-017-1358-1
  21. Thompson JD, Gibson TJ, Plewniak F, et al. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 1997;25:4876-4882. https://doi.org/10.1093/nar/25.24.4876
  22. Stamatakis A, Hoover P, Rougemont J. A rapid bootstrap algorithm for the RAxML Web servers. Syst Biol. 2008;57:758-771. https://doi.org/10.1080/10635150802429642
  23. Tamura K, Peterson D, Peterson N, et al. MEGA5:molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Bio Evol. 2011;28:2731-2739. https://doi.org/10.1093/molbev/msr121
  24. Gannibal PB, Lawrence DP. Distribution of Alternaria species among sections. 3. Sections Infectoriae and Pseudoalternaria. Mycotaxon. 2016;131:781-790. https://doi.org/10.5248/131.781

피인용 문헌

  1. A novel species and a new record of Alternaria isolated from two Solanaceae plants in China vol.18, pp.8, 2018, https://doi.org/10.1007/s11557-019-01504-3
  2. Polyphasic identification of three new species in Alternaria section Infectoriae causing human cutaneous infection vol.63, pp.2, 2018, https://doi.org/10.1111/myc.13026
  3. Morphological and molecular identification of two new Alternaria species (Ascomycota, Pleosporaceae) in section Radicina from China vol.78, pp.None, 2021, https://doi.org/10.3897/mycokeys.78.64853