Mycobiology

  • 제46권3호
  • /
  • Pages.278-282
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  • 2018
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  • 1229-8093(pISSN)
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  • 2092-9323(eISSN)
한국균학회 (The Korean Society of Mycology)
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Morphology and Molecular Characterization of Alternaria argyranthemi on Chrysanthemum coronarium in China

  • Luo, Huan (Department of Plant Protection, College of Agriculture, Yangtze University) ;
  • Xia, Zhen Zhou (Department of Plant Protection, College of Agriculture, Yangtze University) ;
  • Chen, Yun Yun (Plant Protection, College of Plant Protection, Fujian Agriculture and Forestry University) ;
  • Zhou, Yi (Department of Plant Protection, College of Agriculture, Yangtze University) ;
  • Deng, Jian Xin (Department of Plant Protection, College of Agriculture, Yangtze University)
  • 투고 : 2018.04.04
  • 심사 : 2018.06.21
  • 발행 : 2018.09.01
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초록

Chrysanthemum coronarium is an economically important plant in Asia, and used medicinally, ornamentally and as a vegetable. In April 2017, leaf spot disease on C. coronarium was observed in Shiyan, Hubei, China. A single-spore isolate was obtained and identified based on morphology and sequence analysis using four regions (rDNA ITS, GAPDH, $EF-1{\alpha}$, and RPB2). The results indicated that the fungus is Alternaria argyranthemi. The pathogenicity tests revealed that the species could cause severe leaf spot and blight disease on the host. This is the first report of leaf spot disease on C. coronarium caused by A. argyranthemi in the world, which is also a new record of Alternaria species in China.

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참고문헌

  1. Basta A, Pavlovi M, Couladis M, et al. Essential oil composition of the flower heads of Chrysanthemum coronarium L from Greece. Flavour Fragr J. 2007;22:197-200. https://doi.org/10.1002/ffj.1781
  2. Chung K, Park Y. Development of an agrobacterium-mediated transformation system for regenerating garland chrysanthemum (Chrysanthemum coronarium L.). J Plant Biol. 2005;48:136-141. https://doi.org/10.1007/BF03030573
  3. Bar-Eyal M, Sharon E, Spiegel Y. Nematicidal activity of Chrysanthemum coronarium. Eur J Plant Pathol. 2006;114:427-433. https://doi.org/10.1007/s10658-006-0011-7
  4. Haouas D, Cioni PL, Halima-Kamel MB, et al. Chemical composition and bioactivities of three Chrysanthemum essential oils against Tribolium confusum, (du Val) (Coleoptera: Tenebrionidae). J Pest Sci. 2012;85:367-379. https://doi.org/10.1007/s10340-012-0420-7
  5. Hosni K, Hassen I, Sebei H, et al. Secondary metabolites from Chrysanthemum coronarium (Garland) flowerheads: chemical composition and biological activities. Ind Crop Prod. 2013;44:263-271. https://doi.org/10.1016/j.indcrop.2012.11.033
  6. Uematsu S, Kageyama K, Moriwaki J, et al. Colletotrichum carthami comb. nov., an anthracnose pathogen of safflower, garland chrysanthemum and pot marigold, revived by molecular phylogeny with authentic herbarium specimens. J Gen Plant Pathol. 2012;78:316-330. https://doi.org/10.1007/s10327-012-0397-3
  7. Kawaradani M, Taguchi K, Okada K, et al. Seedling rot of garland chrysanthemum caused by Gibellulopsis chrysanthemi and ecological characters of the causal fungus. J Gen Plant Pathol. 2013;79:346-349. https://doi.org/10.1007/s10327-013-0462-6
  8. Hirooka Y, Kawaradani M, Sato T. Description of Gibellulopsis chrysanthemi sp. nov. from leaves of garland chrysanthemum. Mycol Progress. 2014;13:13-19. https://doi.org/10.1007/s11557-012-0887-x
  9. Simmons EG. Alternaria: an identification manual. CBS Biodiversity Series 6. Utrecht: CBS Fungal Biodiversity Centre; 2007.
  10. Cenis JL. Rapid extraction of fungal DNA for PCR amplification. Nucleic Acids Res. 1992;20:2380 https://doi.org/10.1093/nar/20.9.2380
  11. White TJ, Bruns TD, Lee SB, et al. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (Eds.). PCR protocols: a guide to methods and applications. San Diego: Academic Press. 1990. p. 315-322.
  12. Berbee ML, Pirseyedi M, Hubbard S. Cochliobolus phylogenetics and the origin of known, highly virulent pathogens, inferred from its and glyceraldehyde-3-phosphate dehydrogenase gene sequences. Mycologia. 1999;91:964-977. https://doi.org/10.2307/3761627
  13. Carbone I, Kohn LM. A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia. 1999;91:553-556. https://doi.org/10.2307/3761358
  14. Liu YJ, Whelen S, Hall BD. Phylogenetic relationships among ascomycetes: evidence from an RNA Polymerse II Subunit. Mol Biol Evol. 1999;16:1799-1808. https://doi.org/10.1093/oxfordjournals.molbev.a026092
  15. Kumar S, Stecher G, Tamura K. Mega7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol. 2016;33:1870. https://doi.org/10.1093/molbev/msw054
  16. Woudenberg JHC, Groenewald JZ, Binder M, et al. Alternaria redefined. Stud Mycol. 2013;75:171-212. https://doi.org/10.3114/sim0015
  17. Hong SG, Cramer RA, Lawrence CB, et al. Alt a 1 allergen homologs from Alternaria, and related taxa: analysis of phylogenetic content and secondary structure. Fungal Genet Biol. 2005;42:119-129. https://doi.org/10.1016/j.fgb.2004.10.009
  18. Lawrence DP, Gannibal PB, Dugan FM, et al. Characterization of Alternaria isolates from the infectoria species-group and a new taxon from Arrhenatherum, Pseudoalternaria arrhenatheria sp. nov. Mycol Progress. 2014;13:257-276. https://doi.org/10.1007/s11557-013-0910-x
  19. Nishikawa J, Nakashima C. Morphological variation and experimental host range of Alternaria cinerariae. Mycoscience. 2015;56:141-149. https://doi.org/10.1016/j.myc.2014.05.001

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  2. Morpho-molecular characterization of causative agents of wilting, leaf spot, fruit blight and stem canker of okra (Abelmoschus esculentus L.) vol.54, pp.17, 2018, https://doi.org/10.1080/03235408.2021.1917283