Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Effects of Dietary Probiotic Mixture on Growth Performance, Caecal Microorganism and Immune Response in Broiler Chickens under Heat Stress

혼합 생균제가 열 스트레스에 노출된 브로일러의 면역반응, 맹장 미생물과 성장능력에 미치는 효과

  • Song, Young-Han (College of Animal Life Science, Kangwon National University) ;
  • Goh, Yong Gyun (College of Animal Life Science, Kangwon National University) ;
  • Um, Kyung-Hwan (College of Animal Life Science, Kangwon National University) ;
  • Park, Byung-Sung (College of Animal Life Science, Kangwon National University)
  • 송영한 (강원대학교 동물생명과학대학) ;
  • 고용균 (강원대학교 동물생명과학대학) ;
  • 엄경환 (강원대학교 동물생명과학대학) ;
  • 박병성 (강원대학교 동물생명과학대학)
  • Received : 2018.08.02
  • Accepted : 2018.09.19
  • Published : 2018.09.30
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Abstract

This study was investigated the effect of dietary probiotic mixture on blood biomarkers, immune responses, caecal microorganism and productivity in broiler chickens under heat stress (HS). A total of 400 broiler chick males (Ross 308) were divided into four groups of 100 heads each, group C (control, room temperature $25^{\circ}C$), HS (heat stress $33^{\circ}C$) and HSP (HS plus probiotic mixture 500, 750 mg/kg of diet), respectively. Broiler body weight gain, feed intake, feed conversion ratio and immune organ weight increased in the HSP group compared to the HS group. The concentrations of blood IgG and lymphocyte were increased in the HSP group compared to the HS group, and the heterophil:lymphocyte (H:L) ratio, corticosterone concentration and mortality were low. Lactobacillus in the cecum increased in the HSP group compared to the HS group, but was lower in Escherichiacoli (E. coli), coliform bacteria and aerobic bacteria. These results indicate that feeding probitic mixture including three strains such as B. subtilis, S. galilaeus and Sphingobacteriaceae to broiler exposed to heat stress can improve production with lowering mortality by improving immune response and microbial balance.

본 연구는 열 스트레스 (heat stress, HS)에 노출된 브로일러에서 혼합 생균제의 급여가 혈액 생체지표, 면역반응, 맹장 미생물 및 생산성에 미치는 영향을 조사하였다. 총 400마리의 브로일러 수컷 (Ross 308)을 각각 100마리씩 4그룹, C (대조군, 실온 $25^{\circ}C$), HS (열 스트레스 $33^{\circ}C$), HSP (HS플러스 혼합 생균제 500, 750 mg/kg) 그룹으로 배치하였다. 브로일러의 증체량, 사료섭취, 사료요구율 및 면역기관 무게는 HSP 그룹에서 HS 그룹과 비교했을 때 증가하였다. 혈액 IgG, lymphocytes 농도는 HS 그룹에서 HSP그룹과 비교했을 때 증가하였고, heterophil과 lymphocyte(H:L)비율, 코르티코스테론 농도 및 폐사율은 낮았다. 맹장의 Lactobacillus는 HS 그룹과 비교했을 때 HSP 그룹에서 증가하였으나 Escherichia coli (E. coli), coliform bacteria, aerobic bacteria는 낮아졌다. 본 연구결과는 열 스트레스에 노출된 브로일러에게 B. subtilis, S. galilaeus 및 Sphingobacteriaceae등 3가지 균주가 포함된 혼합 생균제를 급여해주면 면역반응 증진, 미생물 균형을 유지해줌으로써 폐사율을 낮추고 생산성을 개선할 수 있음을 시사해준다.

Keywords

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