한국식품과학회지 (Korean Journal of Food Science and Technology)

한국식품과학회 (Korean Society of Food Science and Technology)
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꾸지뽕(Cudrania tricuspidata) 열매에서 분리된 조다당의 큰포식세포 면역 활성 조절

Immunomodulatory activities of polysaccharides extracted from Cudrania tricuspidata fruits in macrophage

  • 조은지 (공주대학교 식품공학과) ;
  • 김이은 (공주대학교 식품공학과) ;
  • 변의홍 (공주대학교 식품공학과)
  • Cho, Eun-Ji (Department of Food Science and Technology, Kongju National University) ;
  • Kim, Yi-Eun (Department of Food Science and Technology, Kongju National University) ;
  • Byun, Eui-Hong (Department of Food Science and Technology, Kongju National University)
  • 투고 : 2018.07.04
  • 심사 : 2018.08.21
  • 발행 : 2018.10.31
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초록

본 연구는 꾸지뽕 열매로부터 분리한 조다당류(CTPS)가 초기 면역반응에 중추적인 역할을 수행하는 큰포식세포에서 활성화를 유도하는지에 관한 여부를 알아보기 위하여 선천 및 적응면역에서 중추적인 역할을 수행하는 큰포식세포에 CTPS를 처리하여 세포 증식률, 산화질소 분비능 및 사이토카인($TNF-{\alpha}$ 및 IL-6) 분비능이 증가되는 것을 확인하였다. 또한 활성화된 큰포식세포의 세포 표면에서 발현되는 CD80과 CD86의 발현과 탐식세포의 항원 제시에 밀접한 관련이 있는 주조직적합성 복합체(MHC class I 및 II)의 발현이 CTPS 처리구에서 유의적으로 증가되는 것으로 관찰되었다. 이러한 산화질소 분비능과 사이토카인의 증가 원인에 관한 면역기전 분석 결과, MAPKs의 인산화와 $NF-{\kappa}B$의 핵 내 이동성을 증가시켜 면역활성을 증가시키는 것으로 관찰되었다.

Macrophages play a crucial role in the host immune defense system. The current study investigated immunomodulatory activities induced by polysaccharides extracted from Cudrania tricuspidata (CTPS) fruits in murine macrophages and their role in signaling pathways. In macrophages, CTPS predominantly induced nitric oxide (NO), tumor necrosis factor-a, and interleukin-6 production. In addition, CTPS significantly up-regulated expression of the macrophage surface marker (CD80/86 and MHC class I/II). These results indicate that polysaccharides extracted from CTPS may potentially play an immunomodulatory role in macrophages via mitogen-activated protein kinases and nuclear factor-B signaling. These findings may be useful in the development of immune enhancing adjuvant materials obtained from natural sources.

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참고문헌

  1. Bios MS. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1200 (1958) https://doi.org/10.1038/1811199a0
  2. Brewer MS, Ikins WG, Harbers CAAZ. TBA values, sensory characteristics, and volatiles in ground fork during long-term frozen storage: Effects of packaging. J. Food Sci. 57: 558-563 (1992) https://doi.org/10.1111/j.1365-2621.1992.tb08042.x
  3. Byun MW, Byun EH. Immunological synergistic effects of combined treatment with herbal preparation (HemoHIM) and red ginseng extracts. J. Korean Soc. Food Sci. Nutr. 44: 182-190 (2015) https://doi.org/10.3746/jkfn.2015.44.2.182
  4. Byun EB, Jang BS, Sung NY, Byun EH. Immunomodulatory activity of crude polysaccharide separated from Cudrania tricuspidata leaf. J. Korean Soc. Food Sci. Nutr. 45: 1099-1106 (2016) https://doi.org/10.3746/jkfn.2016.45.8.1099
  5. Cha JY, Kim HJ, Jun BS, Cho YS. Effects of water-extract of leaves from Morus alba and Cudrania tricuspi data on the lipid concentration of serum and liver in rats. J. Korean Soc. Agric. Chem. Biotechnol. 43: 303-308 (2000)
  6. Chen F, Nakashima N, Kimura I, Kimura M. Hypoglycemic activity and mechanisms of extracts from mulberry leaves (Folium mori) and cortex mori radicis in streptozotocin-induced diabetic mice. Yakugaku Zasshi 115: 476-482 (1995) https://doi.org/10.1248/yakushi1947.115.6_476
  7. Choi, SR, You, DH, Jang I, Ahn MS, Song EJ, Seo, S. Y, Choi MK, Kim YS, Kim MK. Choi DG. Cytotoxicity of methanol extracts from Cudrania tricuspidata Bureau. Korean J. Medicinal Corp Sci. 20: 153-158 (2012) https://doi.org/10.7783/KJMCS.2012.20.3.153
  8. Cobb MH, Goldsmith EJ. Dimerization in MAP-kinase signaling. Trends Biochem. Sci. 25: 7-9 (2000) https://doi.org/10.1016/S0968-0004(99)01508-X
  9. Hano Y, Matsumoto Y, Sun JY. Nomura T. Structures of three new isoprenylated xanthones, cudraxanthones E, F and G. Planta Med. 56: 478-481 (1990) https://doi.org/10.1055/s-2006-961016
  10. Hegde NR, Chevalier MS, Johnson DC. Viral inhibition of MHC class II antigen presentation. Trends Immunol. 24: 278-285 (2003) https://doi.org/10.1016/S1471-4906(03)00099-1
  11. Jang IM. Treatise on Asian Herbal Medicines. Natural Products Science Seoul National University Press, Seoul, Korea (2003)
  12. Kang DG, Hur TY, Lee GM, Oh HC, Kwon TO, Sohn EJ, Lee HS. Effects of Cudrania tricuspidata water extract on blood pressure and renal functions in NO-dependent hypertension. Life Sci. 70: 2599-2609 (2002) https://doi.org/10.1016/S0024-3205(02)01547-3
  13. Khawla BJ, Fatma C, Sameh M, Oumema NE, Claire BH, Raoudha EG. Structural, functional, and antioxidant properties of water-soluble polysaccharides from potatoes peels. Food Chem. 205: 97-105 (2016) https://doi.org/10.1016/j.foodchem.2016.02.108
  14. Kim JY, Jung KS, Jeong HG. Suppressive effects of the kahweol and cafestol on cyclooxygenase-2 expression in macrophages. FEBS Lett. 569: 321-326 (2004) https://doi.org/10.1016/j.febslet.2004.05.070
  15. Kim J, Ryu HS, Shin JH, Kim HS. In vitro and ex vivo supplementation of Houttuynia cordata extract and immunomodulating effect in mice. J. Korean Soc. Food Sci. Nutr. 34: 167-175 (2005) https://doi.org/10.3746/jkfn.2005.34.2.167
  16. Lee IK, Kim CJ, Song KS, Kim HM, Koshino H, Uramoto M, Yoo ID. Cytotoxic benzyl dihydroflavonols from Cudrania tricuspidata. Phytochemistry 41: 213-216 (1996) https://doi.org/10.1016/0031-9422(95)00609-5
  17. Lee JK, Lee MK, Yun YP, Kim Y, Kim JS, Kim YS, Kim K, Han SS, Lee CK. Acemannan purified from Aloevera induces phenotypic and functional maturation of immature dendritic cells. Int. Immunopharmacol. 1: 1275-1284 (2001) https://doi.org/10.1016/S1567-5769(01)00052-2
  18. Mo ZQ, Wang JL, Yang M, Ni LY, Wang HQ, Lao GF, Li YW, Li AX, Luo XC, Dan XM. Characterization and expression analysis of grouper (Epinephelus coioides) co-stimulatory molecules CD83 and CD80/86 post Cryptocaryon irritans infection. Fish Shellfish Immun. 67: 467-474 (2017) https://doi.org/10.1016/j.fsi.2017.05.064
  19. Ottersen T, Vance B, Doorenbos NJ, Chang BL, el-Feraly FS. The crystal structure of cudranone, 2,6,3'-trihydroxy-4-methoxy-2'-(3-methyl-2-butenyl)-benzophenone: a new antimicrobial agent from Cudrania chochinchinensis.Acta. Chem. Scand. B 31: 434-436 (1977)
  20. Piani A, Hossle JP, Birchler T, Siegrist CA, Heumann D, Davies G, Loeliger S, Seger R, Lauener RP. Expression of MHC class II molecules contributes to lipopolysaccharide responsiveness. Eur. J. Immunol. 30: 3140-3146 (2000) https://doi.org/10.1002/1521-4141(200011)30:11<3140::AID-IMMU3140>3.0.CO;2-O
  21. Schepetkin IA, Quinn MT. Botanical polysaccharides: macrophage immunomodulation and therapeutic potential. Int. Immunopharmacol. 6: 317-333 (2006) https://doi.org/10.1016/j.intimp.2005.10.005
  22. Seo MJ, Kang BW, Park JU, Kim MJ, Lee HH, Kim NH, Kim KH, Rhu EJ, Jeong YK. Effect of fermented Cudrania tricuspidata fruit extracts on the generation of the cytokines in mouse spleen cells. J. Life Sci. 23: 682-688 (2013) https://doi.org/10.5352/JLS.2013.23.5.682
  23. Sung NY, Park YY, Kim YE, Cho EJ, Kim MH, Ryu GH, Byun EH, Park YJ. Immuno-modulatory activiies of polysaccharides separated from Chrysanthenuon zawadskii var. latilobum in macrophage cells. Korean J. Food Nutr. 49: 431-437 (2016)
  24. Youn KS, Kim JW. Antioxidant and angiotensin converting enzyme I inhibitory activities of extracts from Mulberry (Cudrania tricuspidata) fruit subjected to different drying methods. J. Korean Soc. Food Sci. Nutr. 41: 1388-1394 (2012) https://doi.org/10.3746/jkfn.2012.41.10.1388
  25. Yu AR, Park HY, Kim YS, Ha SK, Hong HD, Choi HD. Immuno-enhancing effect of seed extracts on a RAW 264.7 macrophage cell line. J. Korean Soc. Food Sci. Nutr. 41: 1671-1676 (2012) https://doi.org/10.3746/jkfn.2012.41.12.1671