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Concentration Range Analysis for Fluorescence Expression of Indocyanine Green

Indocyanine green 형광조영제의 형광발현을 위한 농도 범위 분석

  • Kim, Yong Jae (Dept. of Bionano Technology, College of Bionano Technology, Gachon University) ;
  • Lee, Da Ae (Dept. of Nanophysics, College of Bionano Technology, Gachon University) ;
  • Yoon, Ki-Cheol (Dept. of Premedical, College of Medicine, Gachon University) ;
  • Kim, Kwang Gi (Medical Devices R&D Center, Gachon University Gil Hospital)
  • Received : 2019.08.13
  • Accepted : 2019.11.18
  • Published : 2019.12.31
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Abstract

In the characteristic of the brain malignant, the blood vessels and tumors have the same color and shape, and the boundary distinction is not clear, Therefore, it is difficult to observe the naked eye. Because of the high invasiveness, the risk of recurrence is high. Therefore, complete resection of the tumor is essential. The method for distinguishing the boundary between blood vessels and tumors is a fluorescence contrast method using indocyanine green (ICG), a fluorescence contrast agent. In ICG, the concentration range analysis is very important because the fluorescence expression state varies depending on the concentration. However, since the analysis result of the fluorescence expression condition is insufficient according to the current concentration, this paper proposes by analyzing the initial protocol of the concentration range. 780 nm infrared light was irradiated to the ICG sample to observe the fluorescence expression through a near infrared (NIR) camera. The wavelength is measured by using a spectrum instrument (ocean view) to observe the fluorescence expression wavelength of 811nm. As a result of analyzing the mol concentration according to each sample, the fluorescence expression range was found to be 16.15-0.16M and the optimum fluorescence concentration on the brightest part was found to be 3.23-0.81M.

Keywords

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