Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
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Investigating Survival of Erwinia amylovora from Fire Blight-Diseased Apple and Pear Trees Buried in Soil as Control Measure

토양에 매몰 방제된 화상병 감염 사과와 배 나무로부터 화상병균 생존 조사

  • Kim, Ye Eun (Department of Microbiology and Institute of Biodiversity, College of Natural Science, Dankook University) ;
  • Kim, Jun Young (Department of Microbiology and Institute of Biodiversity, College of Natural Science, Dankook University) ;
  • Noh, Hyeong Jin (Department of Microbiology and Institute of Biodiversity, College of Natural Science, Dankook University) ;
  • Lee, Dong Hyeung (Department of Microbiology and Institute of Biodiversity, College of Natural Science, Dankook University) ;
  • Kim, Su San (Department of Microbiology and Institute of Biodiversity, College of Natural Science, Dankook University) ;
  • Kim, Seong Hwan (Department of Microbiology and Institute of Biodiversity, College of Natural Science, Dankook University)
  • 김예은 (단국대학교 자연과학대학 미생물학과 및 생물다양성연구소) ;
  • 김준영 (단국대학교 자연과학대학 미생물학과 및 생물다양성연구소) ;
  • 노형진 (단국대학교 자연과학대학 미생물학과 및 생물다양성연구소) ;
  • 이동형 (단국대학교 자연과학대학 미생물학과 및 생물다양성연구소) ;
  • 김수산 (단국대학교 자연과학대학 미생물학과 및 생물다양성연구소) ;
  • 김성환 (단국대학교 자연과학대학 미생물학과 및 생물다양성연구소)
  • Received : 2019.09.09
  • Accepted : 2019.11.08
  • Published : 2019.12.31
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Abstract

BACKGROUND: Since 2015, fire blight disease caused by Erwinia amylovora has been devastating apple and pear orchards every year. To quickly block the disease spreading, infected apple and pear trees have been buried in soil. However, concern on the possibility of the pathogen survival urgently requires informative data on the buried host plants. Therefore, this study was conducted to investigate the survival of the pathogen from the buried host plants. METHODS AND RESULTS: Apple trees buried in 42 months ago in a Jecheon site and pear trees buried in 30 months ago in an Anseong site were excavated using an excavator. Plant samples were taken from stems and twigs of the excavated trees. The collected 120 samples were checked for rotting and used for bacterial isolation, using TSA, R2A, and E. amylovora selection media. The purely isolated bacteria were identified based on colony morphology and 16S rDNA sequences. Wood rotting and decay with off smells and discoloring were observed from the samples. A total of 17 genera and 48 species of bacteria were identified but E. amylovora was not detected. CONCLUSION: Our investigation suggests that the survival of E. amylovora doesn't seem possible in the infected hosts which have been buried in soil for at least 30 months. Therefore, the burial control can be considered as a safe method for fire blight disease.

Keywords

References

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