Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Characterization and application of the proteins isolated from edible insects

식용곤충 분리단백의 특성 및 활용연구

  • Kim, Sun-Min (Department of Food and Nutrition, Sangmyung University) ;
  • An, Chae-Won (Department of Food and Nutrition, Sangmyung University) ;
  • Han, Jung-Ah (Department of Food and Nutrition, Sangmyung University)
  • 김선민 (상명대학교 식품영양학과) ;
  • 안채원 (상명대학교 식품영양학과) ;
  • 한정아 (상명대학교 식품영양학과)
  • Received : 2019.09.27
  • Accepted : 2019.11.14
  • Published : 2019.12.31
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Abstract

Proteins were isolated from four species of edible insects, Bombyx mori, B; Tenebrio molitor, T; Protaetia brevitarsis, P; and Gryllus bimaculatus, G, and their properties were analyzed and compared with those of whey proteins. The yield of the protein isolated from raw materials was 65.0-75.0%. Among three branched chain amino acids (BCAA), the amounts of valine in all the insects were similar to that of the whey protein. The BCAA content was 16.8 and 16.4% in G and T, respectively. The total polyphenolic content and the antioxidant activity of the insect proteins were significantly higher than those of the whey proteins. Based on the sensory test of the protein powder, the whey protein could be replaced by 25% of the isolated protein from the insects, but the taste and flavor needs to be improved. Although the BCAA content of insect proteins was lower than that of the whey protein (by 73-76%), their application could be useful because of high polyphenolic content and the anti-oxidative activity.

국내에서 식용으로 유통 및 판매가 가능한 7종의 곤충 중 누에(Bombyx mori), 갈색거저리(Tenebrio molitor) 유충, 흰점박이꽃무지(Protaetia brevitarsis) 유충, 쌍별귀뚜라미(Gryllus bimaculatus)의 4종을 대상으로 단백질을 분리하고, 유청 단백질과 비교하여 항산화 및 영양 특성을 분석하였다. 원물시료에서 분리한 단백질의 수율은 65.0-75.0%로 나타났다. 각 단백질의 아미노산을 분석한 결과, 모든 곤충 시료에서 필수 아미노산인 Phe, Trp은 유청 단백질보다 높은 함량을 보였다. 근육생성에 도움이 되는 세 종류의 BCAA아미노산 중 Val은 모든 곤충단백질에서 유청단백과 유사한 함량을 보였으며, BCAA함량은 곤충분리단백질 중 쌍별 귀뚜라미와 갈색거저리 유충에서 그 함량이 각각 16.8과 16.4%로 가장 높아 유청단백(22.2-23%)의 73-76% 수준인 것으로 나타났다. 곤충 단백질의 총 폴리페놀함량은 유청단백보다 유의적으로 많았으며 곤충단백중에는 누에의 함량이 유의적으로 낮게 측정되었다. 항산화활성 또한 곤충단백이 유청단백보다 유의적으로 높았으며, 특히 갈색거저리 유충에서의 활성이 가장 높게 측정되었다. 관능검사결과에서는 시판 단백질파우더와 동일한 조성으로 제조했을 경우, 유청단백의 25%의 대체가 가능할 것으로 보이나 맛과 향이 대조군에 비해 많이 떨어지므로 보완할 필요가 있다. 곤충단백질은 유청단백질에 비해 BCAA함량은 다소 낮았지만 폴리페놀 함량이 높고 항산화활성이 있으므로 아미노산 조성을 참고하여 특정목적 단백질 제품으로 사용될 가능성이 높음이 확인되었다.

Keywords

References

  1. Ahn MY, Hwang JS, Yoon HJ, Park KHY, Kim SH and Kim EM. Fasting conditions and dietary phenamena of edible cricket (Gryllus bimaculatus). J. Seric. Entomol. Sci. 53: 78-81 (2015) https://doi.org/10.7852/jses.2015.53.2.78
  2. Baek MH, Hwang JS, Kim MA, Kim SH, Goo TW and Yun EY. Comparative analysis of nutritional components of edible insects registered as novel foods. J. Life Sci. 27:334-338 (2017a) https://doi.org/10.5352/JLS.2017.27.3.334
  3. Baek MH, Seo MC, Kim MA, Yun EY and Hwang JS. The antioxidant activities and hair-growth promotion effects of Tenebrio molitor Larvae Extracts (TMEs). J. Life Sci. 27: 1269-1275 (2017b) https://doi.org/10.5352/JLS.2017.27.11.1269
  4. Blois MS. Antioxidant determination by the use of a stable free radical. Nature 26: 1199-1200 (1958) https://doi.org/10.1038/1811199a0
  5. Bukkens SG. The nutritional value of edible insects. Ecol. Food Nutr. 36: 287-319 (1997) https://doi.org/10.1080/03670244.1997.9991521
  6. Choi JH, Kim DI, Park SH, Kim JM, Cho WK, lee KG, Yeo JH, Lee YW. Effects of silkworm (Bombyx mori L.) powder on oxygen radicals and their scavenger enzymes in liver of SD rats. Korean J. Life Sci. 10: 347-353 (2000)
  7. Chung MY, Gwon EY, Hwang JS, Goo TW and Yun EY. Pre-treatment conditions on the powder of Tenebrio molitor for using as a novel food ingredient. J. Life Sci. 51: 664-668 (2013a)
  8. Chung MY, Hwang JS, Goo TW and Yun EY. Analysis of general composition and harmful material of Protaetia brevitarsis. J. Life Sci. 23: 664-668 (2013b) https://doi.org/10.5352/JLS.2013.23.5.664
  9. Churchward-Venne TA, Murphy CH, Longland TM, Phillips SM. Role of protein and amino acids in promoting lean mass accretion with resistance exercise and attenuating lean mass loss during energy deficit in humans. Amino Acids 45: 231-240 (2013) https://doi.org/10.1007/s00726-013-1506-0
  10. da Luz CR, Nicastro H, Zanchi NE, Chaves DF, Lancha AH. Potential therapeutic effects of branched-chain amino acids supplementation on resistance exercise-based muscle damage in humans. J. Int. Soc. Sports Nutr. 8: 23-26 (2011) https://doi.org/10.1186/1550-2783-8-23
  11. Dickinson JM, Gundermann DM, Walker DK, Reidy PT, Borack MS, Drummond MJ, Arora M, Volpi E, Rasmussen BB. Leucineenriched amino acid ingestion after resistance exercise prolongs myofibrillar protein synthesis and amino acid transporter expression in older men. J. Nutr. 144: 1694-1702 (2014) https://doi.org/10.3945/jn.114.198671
  12. Folin O, Denis W. On phosphotungastic-phosphomolybdic compounds as color reagents. J. Biol. Chem. 12: 239-249 (1912) https://doi.org/10.1016/S0021-9258(18)88697-5
  13. Glass DJ. Signaling pathways that mediate skeletal muscle hypertrophy and atrophy. Nat. Cell Biol. 5, 87-90 (2003) https://doi.org/10.1038/ncb0203-87
  14. Hwang SY, Choi SK. Quality characteristics of muffins containing mealworm (Tenebrio molitor). Culi. Sci. Hos. Res. 21: 104-115 (2015) https://doi.org/10.20878/cshr.2015.21.3.009009009
  15. Jeong HJ, Park SB, Kim SA and Kim HK. Total polyphenol content and antioxidative activity of wild grape (Vitis coignetiae) extracts depending on ethanol concentrations. J. Korean Soc. Food Sci. Nutr. 36: 1491-1496 (2007) https://doi.org/10.3746/JKFN.2007.36.12.1491
  16. Kalman D. Amino acid composition of an organic brown rice protein concentrate and isolate compared to soy and whey concentrates and isolates. Foods 3: 394-402 (2014) https://doi.org/10.3390/foods3030394
  17. Kim YM. Quality characteristics of white bread with hot air-dried Tenebrio molitor Larvae Linne powder. Korean J. Food Cook Sci. 33: 513-522 (2017) https://doi.org/10.9724/kfcs.2017.33.5.513
  18. Kim HJ, Kang SJ, Kim SG, Kim JE and Koo HY. Antioxidant activity and antimicrobial activity of the grasshopper, Oxya chinensis sinuosa. J. Seric. Entomol. Sci. 53: 130-134 (2015a) https://doi.org/10.7852/jses.2015.53.2.130
  19. Kim HM, Kim JN, KiM JS, Jeong MY, Yun EY, Hwang JS and Kim AJ. Quality characteristics of patty prepared with mealworm powder. J. Korean Soc. Food Sci. Nutr. 28: 813-820 (2015b) https://doi.org/10.9799/ksfan.2015.28.5.813
  20. Kim SH, Kim KB, Noh JS, Yun EY and Choi SK. Quality characteristics of pasta with addition of Mealworm (Tenebrio molitor). Food Serv. Ind. J. 10: 55-64 (2014)
  21. Kim SA, Lee JA, Kim JM, Kim HA, Kim YA, Yun HJ and Cho YH. Effect of ascorbic acid, silicon, Fe, proline and lysine on proliferation and collagen synthesis in the human dermal fibroblast cell (HS27). J. Korean Soc. Food Sci. Nutr. 38: 1492-1498 (2009) https://doi.org/10.3746/JKFN.2009.38.11.1492
  22. Kwon EY, Yoo JM, Yoon YI, Hwang JS, Goo TW, Kim MY, Choi YC and Yun EY. Pre-treatment of the white-spotted flower chafer (Protaetia brevitarsis) as an ingredient for novel foods. J. Korean Soc. Food Sci. Nutr. 42: 397-402 (2013) https://doi.org/10.3746/JKFN.2013.42.3.397
  23. Lee DG, Kim KB, Choi SK. Quality characteristics of two-spotted cricket (Gryllus bimaculatus), brown stock by high pressure cooking. Culi. Sci. & Hos. Res. 23: 163-174 (2017c). https://doi.org/10.20878/cshr.2017.23.4.016016016
  24. Lee YK, Jeon HR, Jeon BD, Ryu S, Lee SC. Investigations of body composition, nutritional intake and dietary supplementation status before and after weight loss before match in bodybuilders. Korean J. Phy. Edu. 47: 467-474 (2008)
  25. Lee HS, Ryu HJ, Song HJ and Lee SO. Enzymatic preparation and antioxidant activities of protein hydrolysates from Protaetia brevitarsis Larvae. J. Korean Soc. Food Sci. Nutr. 46: 1164-1170 (2017a) https://doi.org/10.3746/jkfn.2017.46.10.1164
  26. Lee KH, Yoon YT, Park YI, Lee HJ and Jeong NY. Quality evaluation of acorn mook prepared with mealworm (Tenebrio molitor) powder. J. Korean Soc. Food Sci. Nutr. 30: 1042-1047 (2017b)
  27. MFDS (Ministry of Food and Drug Safety). Korea food code. Available from: http://www.nifds.go.kr/brd/m_21/view.do?seq=7819&srchFr=&srchTo=&srchWord=&srchTp=&itm_seq_1=0&itm_seq_2=0&multi_itm_seq=0&company_cd=&company_nm=&page=99. Accessed Feb. 19, 2016.
  28. Mishyna M, Martinez JJI, Chen J, Benjamin, O. Extraction, characterization and functional properties of soluble proteins from edible grasshopper (Schistocerca gregaria) and honey bee (Apis mellifera). Food Res. Int. 116: 697-706 (2019) https://doi.org/10.1016/j.foodres.2018.08.098
  29. Oh HG, Lee HY, Kim JH, Kang YR, Moon DI, Seo MY, Back HI, Kim SY, Oh MR, Park SH, Kim MG, Jeon JY, Shin SJ, Ryu KS, Chae SW, Kim O, Park JK. Effects of male silkworm pupa powder on the erectiledys function by chronic hanolconsumption in rats. Lab. Anim. Res. 28: 83-90 (2012) https://doi.org/10.5625/lar.2012.28.2.83
  30. Park KH, Kim GY. Quality and characteristics of manufacturing sunsik with edible insect (Mealworm). Culi. Sci. Hos. Res. 24: 13-23 (2018)
  31. Premalatha M, Abbasi T, Abbasi T, Abbasi SA. Energy-efficient food production to reduce global warming and eco degradation: The use of edible insects. Renew. Sust. Energ. Rev. 15: 4357-4360 (2011) https://doi.org/10.1016/j.rser.2011.07.115
  32. Rural Development Administration. Food composition table. 9th revision edition. Iksan, Chonbuk, Republic of Korea (2015)
  33. Shimomura Y, Murakami T, Nakai N, Nagasaki M, Harris R. Exercise promotes BCAA Catabolism: Effects of BCAA supplementation on skeletal muscle during exercise. J. Nutr. 6: 1583-1587 (2004).
  34. UN. World population prospects: The 2012 Revision. UN, New York, NY, USA. (2013)
  35. Van Huis A, Van Itterbeeck J, Klunder H, Mertens E, Halloran A, Muir G, Vantomme P. Edible Insects: Future prospects for food and feed security (No. 171) Food and Agriculture Organization of the United Nations (2013)
  36. Wagenmakers AJM, Coakley JH, Edwards RHT. Metabolism of branched-chain amino acids and ammonia during exercise clues from McArdle's disease. Int. J. Sport. Med. 11: 103-113 (1990) https://doi.org/10.1055/s-2007-1024771
  37. Yoo JM, Hwang JS, Goo TW and Yun EY. Comparative analysis of nutritional and harmful components in Korean and Chinese mealworms (Tenebrio molitor). J. Korean Soc. Food Sci. Nutr. 42: 249-254 (2013) https://doi.org/10.3746/JKFN.2013.42.2.249
  38. Yu MH, Lee HS, Cho HR, Lee SO. Enzymatic preparation and antioxidant activities of protein hydrolysates from Tenebrio molitor Larvae (mealworm). J. Korean Soc. Food Sci. Nutr. 46: 435-441 (2017) https://doi.org/10.3746/jkfn.2017.46.4.435