Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Pseudomonas syringae pv. tomato DC3000 Improves Escherichia coli O157:H7 Survival in Tomato Plants

  • Namgung, Min (Applied Biology Program, Division of Bioresource Sciences, Kangwon National University) ;
  • Lim, Yeon-Jeong (Applied Biology Program, Division of Bioresource Sciences, Kangwon National University) ;
  • Kang, Min Kyu (Applied Biology Program, Division of Bioresource Sciences, Kangwon National University) ;
  • Oh, Chang-Sik (Department of Horticultural Biotechnology, Kyung Hee University) ;
  • Park, Duck Hwan (Applied Biology Program, Division of Bioresource Sciences, Kangwon National University)
  • Received : 2019.07.29
  • Accepted : 2019.10.04
  • Published : 2019.12.28
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Abstract

Recently, outbreaks of food-borne diseases linked to fresh produce have been an emerging public health concern worldwide. Previous research has shown that when human pathogens co-exist with plant pathogens, they have improved growth and survival rates. In this study, we have assessed whether Escherichia coli O157:H7 benefits from the existence of a phytopathogenic bacterium and the underlying mechanisms were further investigated. When Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) and E. coli O157:H7 were co-inoculated by either dipping or infiltration methods, the populations of E. coli O157:H7 increased; however, no effect was observed when type three secretion system (T3SS) mutants were used instead, suggesting that E. coli O157:H7 benefits from the presence of Pst DC3000. In addition, this study confirmed that the E. coli O157:H7 populations increased when they occupied the tomato leaf intercellular space; this colonization of the interior of the leaves was possible due to the suppression of the PAMP-triggered immunity (PTI) by Pst DC3000, in particular with the AvrPto effector. In conclusion, our data support a plausible model that E. coli O157:H7 benefits from the presence of Pst DC3000 via AvrPto suppression of the PTI resistance.

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References

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