Journal of Animal Reproduction and Biotechnology (한국동물생명공학회지)

The Korean Society of Animal Reproduction and Biotechnology (한국동물생명공학회)
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Establishment of In-Vitro Culture System for Enhancing Production of Somatic Cell Nuclear Transfer (SCNT) Blastocysts with High Performance in the Colony Formation and Formation of Colonies Derived from SCNT Blastocysts in Pigs

  • Han, Na Rae (Department of Animal Life Science, Kangwon National University) ;
  • Baek, Song (Department of Animal Life Science, Kangwon National University) ;
  • Lee, Yongjin (College of Veterinary Medicine, Kangwon National University) ;
  • Lee, Joohyeong (Institute of Veterinary Medicine, Kangwon National University) ;
  • Yun, Jung Im (Institute of Animal Resources, Kangwon National University) ;
  • Lee, Eunsong (College of Veterinary Medicine, Kangwon National University) ;
  • Lee, Seung Tae (Department of Animal Life Science, Kangwon National University)
  • Received : 2019.06.25
  • Accepted : 2019.06.27
  • Published : 2019.06.30
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Abstract

Although somatic cell nuclear transfer (SCNT)-derived embryonic stem cells (ESCs) in pigs have great potential, their use is limited because the establishment efficiency of ESCs is extremely low. Accordingly, we tried to develop in-vitro culture system stimulating production of SCNT blastocysts with high performance in the colony formation and formation of colonies derived from SCNT blastocysts for enhancing production efficiency of porcine ESCs. For these, SCNT blastocysts produced in various types of embryo culture medium were cultured in different ESC culture medium and optimal culture medium was determined by comparing colony formation efficiency. As the results, ICM of porcine SCNT blastocysts produced through sequential culture of porcine SCNT embryos in the modified porcine zygote medium (PZM)-5 and the PZM-5F showed the best formation efficiency of colonies in α-MEM-based medium. In conclusion, appropriate combination of the embryo culture medium and ESC culture medium will greatly contribute to successful establishment of ESCs derived from SCNT embryos.

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References

  1. Baek S, Han NR, Yun JI, Hwang JY, Kim M, Park CK, Lee E, Lee ST. 2017. Effects of Culture Dimensions on Maintenance of Porcine Inner Cell Mass-Derived Cell Self-Renewal. Mol Cells. 40:117-122. https://doi.org/10.14348/molcells.2017.2223
  2. Bogliotti YS, Wu J, Vilarino M, Okamura D, Soto DA, Zhong C, Sakurai M, Sampaio RV, Suzuki K, Izpisua Belmonte JC, Ross PJ. 2018. Efficient derivation of stable primed pluripotent embryonic stem cells from bovine blastocysts. Proc Natl Acad Sci USA. 115:2090-2095. https://doi.org/10.1073/pnas.1716161115
  3. Cha HJ, Yun JI, Han NR, Kim HY, Baek S, Lee SH, Lee J, Lee E, Park CK, Lee ST. 2018. Generation of embryonic stem-like cells from in vivo-derived porcine blastocysts at a low concentration of basic fibroblast growth factor. Reprod Domest Anim. 53:176-185. https://doi.org/10.1111/rda.13088
  4. Cheong SA, Kim E, Kwak SS, Jeon Y, Hyun SH. 2015. Improvement in the blastocyst quality and efficiency of putative embryonic stem cell line derivation from porcine embryos produced in vitro using a novel culturing system. Mol Med Rep. 12:2140-2148. https://doi.org/10.3892/mmr.2015.3634
  5. Fischer K, Kraner-Scheiber S, Petersen B, Rieblinger B, Buermann A, Flisikowska T, Flisikowski K, Christan S, Edlinger M, Baars W, Kurome M, Zakhartchenko V, Kessler B, Plotzki E, Szczerbal I, Switonski M, Denner J, Wolf E, Schwinzer R, Niemann H, Kind A, Schnieke A. 2016. Efficient production of multi-modified pigs for xenotransplantation by 'combineering', gene stacking and gene editing. Sci Rep. 6:29081. https://doi.org/10.1038/srep29081
  6. Hall VJ, Christensen J, Gao Y, Schmidt MH, Hyttel P. 2009. Porcine pluripotency cell signaling develops from the inner cell mass to the epiblast during early development. Dev Dyn. 238:2014-2024. https://doi.org/10.1002/dvdy.22027
  7. Hall VJ and Hyttel P. 2014. Breaking down pluripotency in the porcine embryo reveals both a premature and reticent stem cell state in the inner cell mass and unique expression profiles of the naive and primed stem cell states. Stem Cells Dev. 23:2030-2045. https://doi.org/10.1089/scd.2013.0502
  8. Hou DR, Jin Y, Nie XW, Zhang ML, Ta N, Zhao LH, Yang N, Chen Y, Wu ZQ, Jiang HB, Li YR, Sun QY, Dai YF, Li RF. 2016. Derivation of Porcine Embryonic Stem-Like Cells from In Vitro-Produced Blastocyst-Stage Embryos. Sci Rep. 6:25838. https://doi.org/10.1038/srep25838
  9. Jung SK, Kim HJ, Kim CL, Lee JH, You JY, Lee ES, Lim JM, Yun SJ, Song JY, Cha SH. 2014. Enhancing effects of serum-rich and cytokine-supplemented culture conditions on developing blastocysts and deriving porcine parthenogenetic embryonic stem cells. J Vet Sci. 15:519-528. https://doi.org/10.4142/jvs.2014.15.4.519
  10. Lee SG, Park JK, Choi KH, Son HY, Lee CK. 2015. Embryo Aggregation Promotes Derivation Efficiency of Outgrowths from Porcine Blastocysts. Asian-Australas J Anim Sci. 28:1565-1572. https://doi.org/10.5713/ajas.15.0462
  11. Lee Y, Lee H, Park B, Elahi F, Lee J, Lee ST, Park CK, Hyun SH, Lee E. 2016. Alpha-linolenic acid treatment during oocyte maturation enhances embryonic development by influencing mitogen-activated protein kinase activity and intraoocyte glutathione content in pigs. J Anim Sci. 94:3255-3263. https://doi.org/10.2527/jas.2016-0384
  12. Mujibi FD, Okoth E, Cheruiyot EK, Onzere C, Bishop RP, Fevre EM, Thomas L, Masembe C, Plastow G, Rothschild M. 2018. Genetic diversity, breed composition and admixture of Kenyan domestic pigs. PLoS One. 13:e0190080. https://doi.org/10.1371/journal.pone.0190080
  13. Perleberg C, Kind A, Schnieke A. 2018. Genetically engineered pigs as models for human disease. Dis Model Mech. 11:dmm030783. https://doi.org/10.1242/dmm.030783
  14. Rubessa M, Polkoff K, Bionaz M, Monaco E, Milner DJ, Holllister SJ, Goldwasser MS, Wheeler MB. 2017. Use of Pig as a Model for Mesenchymal Stem Cell Therapies for Bone Regeneration. Anim Biotechnol. 28:275-287. https://doi.org/10.1080/10495398.2017.1279169
  15. Singh VK, Thrall KD, Hauer-Jensen M. 2016. Minipigs as models in drug discovery. Expert Opin Drug Discov. 11:1131-1134. https://doi.org/10.1080/17460441.2016.1223039
  16. Siriboon C, Lin YH, Kere M, Chen CD, Chen LR, Chen CH, Tu CF, Lo NW, Ju JC. 2015. Putative porcine embryonic stem cell lines derived from aggregated four-celled cloned embryos produced by oocyte bisection cloning. PLoS One. 10:e0118165. https://doi.org/10.1371/journal.pone.0118165
  17. Son HY, Kim JE, Lee SG, Kim HS, Lee E, Park JK, Ka H, Kim HJ, Lee CK. 2009. Efficient Derivation and Long Term Maintenance of Pluripotent Porcine Embryonic Stem-like Cells. Asian-Australas J Anim Sci. 22:26-34. https://doi.org/10.5713/ajas.2009.80343
  18. Telugu BP, Ezashi T, Sinha S, Alexenko AP, Spate L, Prather RS, Roberts RM. 2011. Leukemia inhibitory factor (LIF)-dependent, pluripotent stem cells established from inner cell mass of porcine embryos. J Biol Chem. 286:28948-28953. https://doi.org/10.1074/jbc.M111.229468
  19. Vackova I, Ungrova A, Lopes F. 2007. Putative embryonic stem cell lines from pig embryos. J Reprod Dev. 53:1137-1149. https://doi.org/10.1262/jrd.19108
  20. Walters EM and Prather RS. 2013. Advancing swine models for human health and diseases. Mo Med. 110:212-215.
  21. Wang WH, Day BN, Wu GM. 2003. How does polyspermy happen in mammalian oocytes? Microsc Res Tech. 61:335-341. https://doi.org/10.1002/jemt.10346
  22. Whyte JJ and Prather RS. 2011. Genetic modifications of pigs for medicine and agriculture. Mol Reprod Dev. 78:879-891. https://doi.org/10.1002/mrd.21333
  23. Xue B, Li Y, He Y, Wei R, Sun R, Yin Z, Bou G, Liu Z. 2016. Porcine Pluripotent Stem Cells Derived from IVF Embryos Contribute to Chimeric Development In Vivo. PLoS One. 11:e0151737. https://doi.org/10.1371/journal.pone.0151737
  24. Yamanaka K, Sugimura S, Wakai T, Kawahara M, Sato E. 2009. Difference in sensitivity to culture condition between in vitro fertilized and somatic cell nuclear transfer embryos in pigs. J Reprod Dev. 55:299-304. https://doi.org/10.1262/jrd.20174
  25. Yoshioka K, Suzuki C, Tanaka A, Anas IM, Iwamura S. 2002. Birth of piglets derived from porcine zygotes cultured in a chemically defined medium. Biol Reprod. 66:112-119. https://doi.org/10.1095/biolreprod66.1.112