Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Antimelanogenic effect and whitening of crude polysaccharide fraction extracted from Perilla frutescens Britton var. acuta Kudo

자소엽(Perilla frutescens Britton var. acuta Kudo) 조다당의 멜라닌 생성 저해 및 미백효과

  • Cho, Eun-Ji (Department of Food Science and Technology, Kongju National University) ;
  • Byun, Eui-Hong (Department of Food Science and Technology, Kongju National University)
  • 조은지 (공주대학교 식품공학과) ;
  • 변의홍 (공주대학교 식품공학과)
  • Received : 2018.10.17
  • Accepted : 2019.01.22
  • Published : 2019.02.28
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Abstract

In this study, the inhibitory effects of crude polysaccharide fractions separated from Perilla frutescens Britton var. acuta Kudo (PCP) on melanin synthesis and tyrosinase activity were observed. B16F10 melanoma cells were treated with 125 and $250{\mu}g/mL$ of PCP for 24 hours. Using these optimal concentrations, inhibition of melanin synthesis inhibition was measured, and PCP treatment significantly reduced melanin synthesis induced by 3-isobutyl-1-methylxanthine (IBMX). In addition, western blotting analysis on B16F10 melanoma cells showed that PCP inhibited tyrosinase, microphthalmia-associated transcriptipn factor, tyrosinase related protein-1, and tyrosinase related protein-2 expression. Therefore, these results indicate that PCP may have potential inhibitory activity against melanin synthesis and may be a natural ingredient useful for the development of whitening materials in cosmetics and functional foods.

본 연구에서 자소엽 조다당 추출물의 미백 활성을 평가하기 위하여 tyrosinase 억제활성과 melanocyte 세포에서 멜라닌 함량 및 멜라닌 관련 단백질 발현 억제능을 수행하였다. 자소엽 조다당 추출물은 tyrosinase 억제능을 관찰한 결과 자소엽 조다당 추출물의 농도가 증가할수록 활성이 유의적으로 증가하는 것으로 나타났다. 이러한 미백 효과가 melanoma 세포인 B16F10 세포 내에서도 일어나는지 확인하기 위하여 세포내 멜라닌 함량 및 tyrosinase 억제능을 살펴보았다. 자소엽 조다당 추출물은 B16F10 세포에 대하여 세포독성이 없는 농도에서 진행 하였으며, 세포내 멜라닌 함량 및 tyrosinase 활성 저해를 보여주었다. 이는 자소엽 조다당 추출물이 세포 내 MITF 발현을 억제시킴으로서 tyrosinase, TRP-1 및 TRP-2 단백질 발현을 억제함에 따라 미백효과를 확인하였다. 이러한 결과로 자소엽 조다당 추출물의 미백 활성을 확인 할 수 있었으며, 미백 산업 분야 및 식품의 기능성 소재로 이용한다면 천연 미백 소재로써 활용될 수 있을 것으로 판단된다.

Keywords

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Fig. 1. Tyrosinase inhibition activity of crude polysaccharide fraction separated from Perilla frutescens Britton var. acuta Kudo (PCP).

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Fig. 2. Cell viability of IBMX and crude polysaccharide fraction separated from Perilla frutescens Britton var. acuta Kudo (PCP) in B16F10 melanoma cells.

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Fig. 3. The difference of melanin octents in B16F10 mouse melanoma cells treated with crude polysaccharide fraction separated from Perilla frutescens Britton var. acuta Kudo (PCP).

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Fig. 4. Effect of crude polysaccharide fraction separated from Perilla frutescens Britton var. acuta Kudo (PCP) on cellular melanin contents and tyrosinase activity in B16F10 cells.

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Fig. 5. Inhibitory effect of fractions from crude polysaccharide fraction separated from Perilla frutescens Britton var. acuta Kudo (PCP) on TRP-1, TRP-2, MITF, Tyrosine.

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