Journal of Environmental Health Sciences (한국환경보건학회지)

Korean Society of Environmental Health (한국환경보건학회)
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Antioxidative Effect of Typha orientalis L. Extract on the Oxidative Stress Induced by Cytotoxicity of Cadmium Sulfate

황산카드뮴독성의 산화적 손상에 대한 부들 추출물의 항산화 효과

  • Yoon, Ki Chul (Department of Digestive Internal Medicine, Sanbon Hospital, School of Medicine, Wonkwang University) ;
  • Sohn, Young Woo (Department of Digestive Internal Medicine, Sanbon Hospital, School of Medicine, Wonkwang University)
  • 윤기철 (원광대학교 의과대학 산본병원 소화기내과) ;
  • 손영우 (원광대학교 의과대학 산본병원 소화기내과)
  • Received : 2019.01.31
  • Accepted : 2019.02.20
  • Published : 2019.02.28
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Abstract

Objectives: This study was carried out to analyze the cytotoxicity of cadmium sulfate ($CdSO_4$) and the antioxidative effect of Typha orientalis L. (TO) extract on the oxidative stress induced by cytotoxicity of $CdSO_4$ in the cultured NIH3T3 fibroblasts. Methods: For this study, the cell viability and the antioxidative effects such as the inhibitory activity of lipid peroxidation (LP) and superoxide dismutase (SOD)-like activity and xanthine oxidase (XO)-inhibitory activity were assessed. Results: The cadmium sulfate significantly decreased cell viability in dose-dependently, and $XTT_{50}$ value was measured at $47.4{\mu}M$ of $CdSO_4$. The cytotoxicity of $CdSO_4$ was determined as highly toxic by Borenfreund and Puerner's toxic criteria. The butylated hydroxytoluene (BHT) as antioxidant significantly increased cell viability injured by $CdSO_4$-induced cytotoxicity in these cultures. In the protective effect of TO extract on $CdSO_4$-induced cytotoxicity, TO extract remarkably increased the inhibitory ability of LP and XO as well as SOD-like ability. Conclusions: From the above results, it is suggested that the oxidative stress is involved in the cytotoxicity of $CdSO_4$, and TO extract effectively protected $CdSO_4$-induced cytotoxicity by antioxidative effects. The natural component like TO extract may be a putative therapeutic agent for treatment of the toxicity induced by heavy metallic compound like $CdSO_4$ correlated with the oxidative stress.

Keywords

References

  1. Jang S, Lee AR, Choi GY, Kim HK. Monitoring of heavy metal contents incommercial herbal medicines cultivated herbal medicines in the Seoul and Daegu areas. J Environ Health Sci. 2015; 41(1): 30-39.
  2. Kim TY, Jekal SJ. Antioxidative effect of Chelidonium majus extract on cultured NIH3T3 fibroblasts injured by cadmium chloride of toxicant. Kor J Clin Lab Sci. 2016; 48(1): 1-7. https://doi.org/10.15324/kjcls.2016.48.1.1
  3. Jung JY, Joe DY, Park SH, Seo YM. Protective effect of Rumex crispus L. extract on cultured NIH3T3 fibroblasts damaged by cadmium of environmental pollutant. J Kor Soc People Plants Environ. 2014; 17(1): 15-21. https://doi.org/10.11628/ksppe.2014.17.1.015
  4. Jin T, Nordberg GT. cadmium toxicity in kidney cells. resistance induced by short term pretreatment in vitro and in vivo. Acta Pharmacol et Toxicol. 1983; 58(3): 137-143.
  5. Son YW, Rim YS, Yu YW, Jung I. Effect of persimon leaves extract on the cytotoxicity induced by cadmium of hair dye component. J Invest Cosm. 2012; 8(1); 9-15. https://doi.org/10.15810/jic.2012.8.1.002
  6. Choi MK, Chung YT. A study on the cytotoxic effect of cadmium, copper, mercury and chromium on cultured mouse fibroblasts. J Wonkang Med Sci. 1990; 6(1): 77-91.
  7. Son YW, Choi YS. Protection of chenopodium album var. centrorubrum extract by oxidative effect in cultured human skin fibroblasts damaged by cadmium. J Kor Soc People Plants Environ. 2012; 15(3): 155-161.
  8. Son YW, Rim YS, Seo YM. Protective effect of NMDA receptor antagonist on the neurotoxicity induced by lead as an environmental pollutant. J Kor Soc Occup Environ Hyg. 2017; 27(3); 193-200. https://doi.org/10.15269/JKSOEH.2017.27.3.193
  9. Jung JY, Oh SK, Park SH, Yoon MY, Yu YW, Rim YS, Jung IJ. Antioxidative effect of Ajuga multiflora BUNGE extract on chromium trioxide, dermatitis inducer in cultured NIH3T3 fibroblasts. J Invest Cosm. 2014; 10(1): 193-199.
  10. Li YL, Gan GP, Zhang HZ, Wu HZ, Li CL, Hung YP, et al. A flavonoid glycoside isolated from Smilax china L. rhizome in vitro anticancer effects on human cancer cell lines. J Ethnopharmacol. 2007; 113(1): 115-124. https://doi.org/10.1016/j.jep.2007.05.016
  11. Krizkova L, Nagy M, Polonyi J, Dobias J, Belicova A, Grancai D, Krajcovic J. Phenolic acids inhibit chloroplast mutagenesis in Euglena gracillis. Mutat Res. 2000; 469(1): 107-114. https://doi.org/10.1016/S1383-5718(00)00059-0
  12. Fujisawa S, Kadoma Y. Anti-and pro-oxidant effects of oxidized quercetin, curcumin-related compounds with thiols or ascorbate as measured by the induction priod method. In Vitro. 2006; 20(1): 39-44.
  13. Gates MA, Tworoger SS, Hecht JL, De Vivo I, Rosner B, Hankinson SE. A prospective study of dietary flavonoid intake and incidence of epithelial ovarian cancer. Int J Cancer. 2007; 121(10): 2225-2232. https://doi.org/10.1002/ijc.22790
  14. Jung JY, Jung IJ, Jekal SJ. The protective effect of Lonicerae flos extract on cultured C6 glioma cells damaged by aluminum of dementia induced. Kor J Clin Lab Sci. 2017; 49(3): 271-278. https://doi.org/10.15324/kjcls.2017.49.3.271
  15. Tsou TC, Lai HJ, Yang JL. Effects of mannitol or catalase on the generation of reactive oxygen species leading to DNA damaged by chromium (VI) reduction with ascorbate. Hem Res Toxicol. 1999; 12(10); 1002-1009. https://doi.org/10.1021/tx9802264
  16. Seo YM, Kim NS. Effect of superoxide dismitase on oxidative stress of reactive oxygen species in cultured human skin melanocyte. J Kor Soc Occp Environ Hyg. 2009; 19(3): 261-269.
  17. Lim JA, Dh HJ, Back SH. Antiaging ability of methanol extract from Euonymus alatus. J Cosm & Public Health. 2007; 3(2): 41-45.
  18. Han MR, Kim NW, Lee YS. Anti-wrinkle antioxidative effects of ethanol extracts of Inula flos, Chrysanthemi flos and Carthami flos. J Invest Cosm. 2013; 9(4): 361-369.
  19. Kim SJ, Yu YW, Lee JK. Cytotoxicity and protective effect of Portulaca oleracea L. extract on cultured neuroglioma cells damaged by alumimum of dementia-induced agent. J Kor People Plants Environ. 2013; 16(5): 251-256. https://doi.org/10.11628/ksppe.2013.16.5.251
  20. Mosmann, T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Meth. 1983; 65(1-2): 55-63. https://doi.org/10.1016/0022-1759(83)90303-4
  21. A.O.A.C. Official method of analysis. 18th ed., Association of official analytical chemists. Washington DC USA. 2005; 45(3): 21-21.
  22. Nieva Moreno MI, Isla MI, Sampietro AR, Vattuone MA. Comparison of the three radical-scavenging activity of propolis from several regions of Argentina. J Ethmopharmacol 2000; 71(5): 109-114. https://doi.org/10.1016/S0378-8741(99)00189-0
  23. Kikuzaki H, Nakatani N. Antioxidant effects of some ginger constituents. J Food Sci, 1993, 58(6): 1410-1420.
  24. Marklund S, Marklund G. Involement of superoxide anion radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem. 1974; 47(3): 468-474.
  25. Stirpe F, Corte ED. The regulation of rat liver xanthine oxidase. J Biol & Chem. 1969; 244(23): 3855-3861. https://doi.org/10.1016/S0021-9258(17)36428-1
  26. Kong MR, Lee YS. Physiological activity of Equisetum arvense fertile stems extracts as beauty materials. J Invest Cosm. 2014; 10(2): 119-127.
  27. Jo EH, Lee JW, Park SY, Kim PP, Choi KH, Eom IC. Pre-validation of colony forming efficiency assay for assessing the cytotoxicity of nanomaterials. J Environ Health Sci. 2015; 41(1): 17-23.
  28. Kim JK, Seo JK, Kim TS, Park GH. Risk assessment for non-cancer effects of volatile organic compounds in childrens'products. J Environ Health Sci. 2014; 40(3): 178-186.
  29. Borenfreund, E., and J.A. Puerner. A simple quantitative procedure using monolayer culture for cytotoxicity assay (HTD/NR-90). J Tiss Cult Meth. 1984; 9(1): 7-9. https://doi.org/10.1007/BF01666038
  30. Ma J, Luo XD, Protiva P, Tang H, Ma C, Basile MJ, et al. Bioactive novel polyphenols from the fruit of Manikara zapota (Sapodolla). J Nat Rrod. 2003; 66(7): 983-986.
  31. Lee SH, Seo YM. Alleviating effects of Euphorbiae humifusae L. extract on the neurotoxicity induced by lead. Kor J Clin Lab Sci. 2018; 50(4): 501-510. https://doi.org/10.15324/kjcls.2018.50.4.501
  32. Lee SH, Jung IJ, Jang HS. The antioxidative effect of Eclipta prostrata L. extract on cultured NIH3T3 fibroblasts injured by manganese-induced cytotoxicity. Biomed Sci Lett. 2018; 24(4): 1-8. https://doi.org/10.15616/BSL.2018.24.1.1
  33. Wang L, Tu YC, Lian TW, Hung JT, Yen JH, Wu MJ. Distingtive antioxidant and antiinflammatory effects of flavonoids. J Agric Food Chem. 2006; 54(26); 9798-9804. https://doi.org/10.1021/jf0620719