Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Physiological activity of the extract from Dolwoe (Gynostemma pentaphyllum Makino) leaves tea by different ethanol concentrations

에탄올 농도에 따른 돌외 잎 차 추출물의 생리활성

  • Kim, Kyeoung Cheol (Major in Plant Resource and Environment, SARI, Jeju National University) ;
  • Kim, Ju-Sung (Major in Plant Resource and Environment, SARI, Jeju National University)
  • 김경철 (제주대학교 식물자원환경전공) ;
  • 김주성 (제주대학교 식물자원환경전공)
  • Received : 2018.10.30
  • Accepted : 2018.11.30
  • Published : 2019.03.31
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Abstract

The objective of this study was to compare the effects of different concentration of ethanol extraction on the total phenol and flavonoid contents and physiological activities. The total phenol content of the extracts ranged from 35.54 to 71.52 mg GAE/g. An increase in the ethanol concentration of the solvent led to an increase in the phenol content, with the highest content being found in the 80 and 99.5% ethanol extract. The same trend was observed for flavonoid content. DPPH, nitric oxide, superoxide, hydroxyl radical scavenging activity and TEAC, FRAP and ORAC were measured by antioxidant assay. Radical scavenging activity of aqueous ethanol extracts was better than that of water and 99.5% ethanol extracts. TEAC and FRAP were highly dependent on ethanol concentration and ORAC showed high activity in 40 ~ 80% ethanol extract. Antioxidant activity of Dolwoe leaves tea showed different results among the assay systems. In most experiments, the activities of water and 99.5% ethanol extracts was relatively low. ${\alpha}$-Glucosidase inhibitory activity and microorganism inhibitory activity were highest in the 80% and 99.5% ethanol extracts. Therefore, it was considered that extraction with 80% ethanol was appropriate when considering the antioxidative and physiological activities of Dolwoe leaves tea. Based on these results, it can be used as a basic data for the development of food of Dolwoe leaves tea.

본 연구는 페놀 및 플라보노이드의 총 함량과 생리 활성에 대한 다양한 농도의 에탄올 추출 효과를 비교하기 위해 수행하였다. 추출물의 총 페놀 함량은 35.54 ~ 71.52 mg GAE / g 범위였다. 용매의 에탄올 농도가 증가함에 따라 페놀 함량이 높았고 80%와 99.5%의 에탄올 추출물에서 최고 함량이 확인되었다. 추출물의 총 플라보노이드 함량은 페놀 함량의 경향과 유사하였다. 항산화 실험으로 DPPH, nitric oxide, superoxide, hydroxyl radical 소거능과 TEAC, FRAP, ORAC를 측정하였다. Radical 소거능은 물이나 99.5% 에탄올 추출물 보다 에탄올 수용액 추출물의 활성이 우수하였다. TEAC와 FRAP은 에탄올 농도 의존적인 경향으로 높은 값이 나타났고 ORAC는 40 ~ 80% 에탄올 추출물이 높은 활성을 보였다. 돌외 잎 차의 항산화능을 검토한 결과 측정 방식에 따라 다른 경향이 확인되었다. 그리고 대부분의 실험에서 물 또는 99.5% 에탄올 추출물의 활성이 상대적으로 낮았다. ${\alpha}$-Glucosidase 저해활성은 80%, 99.5% 에탄올 추출물에서 가장 높은 활성이 확인되었고, 미생물 저해활성 또한 동일한 결과를 보였다. 따라서 차의 항산화 활성과 생리활성을 복합적으로 고려할 때 80% 에탄올로 추출하는 것이 적절한 것으로 사료된다. 이러한 결과를 토대로 돌외 차의 식품개발에 있어 기초자료로 활용될 수 있다고 생각된다.

Keywords

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