The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Effect on Colony Growth Inhibition of Soil-Borne Fungal Pathogens by Available Chlorine Content in Sodium Hypochlorite

  • Lee, Sung-Hee (Bureau of Research & Development, Chungcheongbuk-do Agricultural Research and Extension Services) ;
  • Shin, Hyunman (Bureau of Research & Development, Chungcheongbuk-do Agricultural Research and Extension Services) ;
  • Kim, Ju-Hyoung (Bureau of Research & Development, Chungcheongbuk-do Agricultural Research and Extension Services) ;
  • Ryu, Kyoung-Yul (Department of Agro-food Safety and Crop Protection, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Kim, Heung Tae (Department of Plant Medicine, Chungbuk National University) ;
  • Cha, Byeongjin (Department of Plant Medicine, Chungbuk National University) ;
  • Cha, Jae-Soon (Department of Plant Medicine, Chungbuk National University)
  • Received : 2018.07.04
  • Accepted : 2018.12.09
  • Published : 2019.04.01
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Abstract

Our study investigated the available chlorine content, contact time and difference among strains of each pathogen for sodium hypochlorite (NaOCl) to control chemically against soil-borne fungal pathogens, such as Phytophthora rot by Phytophthora cactorum, violet root rot by Helicobasidium mompa, and white root rot by Rosellinia necatrix, causing die-back symptom on apple trees. As a result, the colony growth of Phytophthora cactorum was inhibited completely by soaking over 5 s in 31.25 ml/l available chlorine content of NaOCl. Those of H. mompa and R. necatrix were inhibited entirely by soaking over 160 s in 62.5 and 125 ml/l available chlorine content in NaOCl, respectively. Also, inhibition effect on available chlorine in NaOCl among strains of each soil-borne pathogen showed no significant difference and was similar to or better than that of fungicides.

Keywords

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Fig. 1. Representative colony growth inhibition of soil-borne fungal pathogens causing apple tree dieback by available chlorine content in NaOCl on PDA. Each treated available chlorine content in NaOCl was 12.5 (left), 31.25, 62.5 and 125 ml/l on plate (A), (B) and (C), clockwise. The center agar plug on all plates was a control. Test plate A, B and C were for Phytophthora cactorum KACC40166, Helicobasidium mompa KACC40836 and Rosellinia necatrix KACC40168 isolate, respectively.

Table 1. Colony inhibition rate for a typical isolate of soil-borne fungal pathogens by available chlorine content of NaOCl on PDA

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Table 2. Colony inhibition rate for a typical isolate of soil-borne fungal pathogens by soaking time from the determined available chlorine content of NaOCl

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Table 3. Colony inhibition rate of 6 isolates for Phytophthora cactorum by available chlorine content of NaOCl and a fungicide

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Table 4. Colony inhibition rate of 3 isolates for Helicobasidium mompa and Helicobasidium sp. by available chlorine content of NaOCl and 2 fungicides

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Table 5. Colony inhibition rate of 6 isolates for Rosellinia necatrix and Rosellinia sp. by available chlorine content of NaOCl and 3 fungicides

E1PPBG_2019_v35n2_156_t0010.png 이미지

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