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유산균을 이용한 흰목이 버섯의 발효를 통한 항비만과 항당뇨 효과

Anti-obesity and Anti-diabetes Effects of the Fermented White Jelly Fungus (Tremella fuciformis Berk) Using Lactobacillus rhamnosus BHN-LAB 76

  • 이준형 (비에이치앤바이오 기업부설 생물산업소재개발연구소) ;
  • 김병혁 (비에이치앤바이오 기업부설 생물산업소재개발연구소) ;
  • 윤여초 (비에이치앤바이오 기업부설 생물산업소재개발연구소) ;
  • 김중규 (비에이치앤바이오 기업부설 생물산업소재개발연구소) ;
  • 박예은 (비에이치앤바이오 기업부설 생물산업소재개발연구소) ;
  • 박혜숙 (비에이치앤바이오 기업부설 생물산업소재개발연구소) ;
  • 황학수 (교촌 F&B) ;
  • 권인숙 (안동대학교 식품영양학과) ;
  • 권기석 (안동대학교 원예.생약융합학부) ;
  • 이중복 (비에이치앤바이오 기업부설 생물산업소재개발연구소)
  • Lee, Jun-Hyeong (Institute for Development of Bioindustrial Materials, BHNBIO Co., LTD.) ;
  • Kim, Byung-Hyuk (Institute for Development of Bioindustrial Materials, BHNBIO Co., LTD.) ;
  • Yoon, Yeo-Cho (Institute for Development of Bioindustrial Materials, BHNBIO Co., LTD.) ;
  • Kim, Jung-Gyu (Institute for Development of Bioindustrial Materials, BHNBIO Co., LTD.) ;
  • Park, Ye-Eun (Institute for Development of Bioindustrial Materials, BHNBIO Co., LTD.) ;
  • Park, Hye-Suk (Institute for Development of Bioindustrial Materials, BHNBIO Co., LTD.) ;
  • Hwang, Hak-Soo (Kyochon F&B Co., LTD.) ;
  • Kwun, In-Sook (Department of Food Science and Nutrition, Andong National University) ;
  • Kwon, Gi-Seok (Divistion of Horticulture & Medicinal Plant, Andong National University) ;
  • Lee, Jung-Bok (Institute for Development of Bioindustrial Materials, BHNBIO Co., LTD.)
  • 투고 : 2018.11.27
  • 심사 : 2019.04.19
  • 발행 : 2019.04.30

초록

흰목이 버섯(Tremella fuciformis Berk)은 고혈압, 당뇨병, 비만, 폐질환, 미용에 효과가 있으며, 고대 중국에서는 불로장생약이라고 알려져 있다. 본 연구에서는 생물전환공정을 통해 발효된 흰목이 버섯 추출물의 항비만 및 당뇨병 효과를 조사하였다. 우리는 비만 유도 시킨 C57BL / 6N 마우스에 High Fats Diet (HFD), 5% non-fermented Tremella fuciformis (TF), 2.5% fermented Tremilla fuciformis (FTF), 5% FTF를 8주간 투여하였다. 경구당 부하검사는 7주간 식이섭취 후 실시하였으며, 8주간 식이섭취 후 섭취량, 식이섭취효율, 체중, 간, 부고환 지방량 및 혈청 인슐린 수치 및 HOMA-IR을 분석하였다. 또한, 혈청 총콜레스테롤 함량 및 중성지방 함량을 분석하였다. FTF (실험)군과 TF (실험)군 및 HFD (실험)군과 비교한 결과 5% FTF (실험)군이 체중, 조직 중량, 중성지방 함량 및 HOMA-IR을 감소시키는 것을 확인하였다. 이 결과를 통해 발효 흰목이 버섯은 항비만 효과를 확인하였다. 마지막으로, 본 연구는 발효 흰목이 버섯을 이용한 비만 치료의 기초자료로 사용하고자 한다.

White jelly fungus (Tremella fuciformis Berk) is effective for hypertension, diabetes, obesity, lung disease, beauty, and has been known as an elixir of life in ancient Chinese dynasty. In this study, we investigated the anti-obesity and diabetic effects of the fermented white jelly fungus extract by the bioconversion process. We fed an obesity-inducing mouse with 5% non-fermented Tremella fuciformis (TF), 2.5% fermented Tremella fuciformis (FTF), and 5% FTF containing High Fats Diet (HFD) and HFD for 8 weeks, respectively. The oral glucose tolerance test (OGTT) was performed analysis after 7 weeks of feeding and the dietary intake, food efficiency ratio, body weight, liver, epididymal fat weight, and serum insulin level were measured after 8 weeks of feeding. Also, HOMA-IR was analyzed. The concentrations of serum total cholesterol, triglycerides test was analyzed. The FTF compare with 5% TF and HFD confirmed that 5% FTF reduced body weight, tissue weight, triglycerides concentration, HOMA-IR, respectively. As a result, we confirmed that the fermented white jelly fungus has the anti-obesity effect. Finally, this study can be used a basic data for obesity treatment using fermented white jelly fungus.

키워드

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Fig. 1. Liver and epididymal fat weight of mice fed experimental diets supplemented with fermented White jelly fungus (Tremella fuciformis Berk) at 1% of diets for 8 weeks.

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Fig. 2. AUC (Area under the curve) calculation after glucose treatment in mice fed experimental diets supplemented with fermented White jelly fungus (Tremella fuciformis Berk) at 1% of diets for 8 weeks.

Table 1. Composition of experimental diets

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Table 2. Effect of fermented white jelly fungus (Tremella fuciformis Berk) powder on final body weight, food intake and food efficiency ratio

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Table 3. The lipid profiles of each experimental group after 8 weeks

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Table 4. Effects of the fermented white jelly fungus (Tremella fuciformis Berk) on serum glucose and insulin levels in mouse

SMGHBM_2019_v29n4_470_t0004.png 이미지

참고문헌

  1. Ahmed, F. E. 2003. Genetically modified probiotics in foods. Trends Biotechnol. 21, 491-497. https://doi.org/10.1016/j.tibtech.2003.09.006
  2. Cheng, H. H., Hou, W. C. and Lu, M. L. 2002. Interactions of lipid metabolism and intestinal physiology with Tremella fuciformis Berk edible mushroom in rats fed a high-cholesterol diet with or without Nebacitin. J. Agric. Food Chem. 50, 7438-7443. https://doi.org/10.1021/jf020648q
  3. Cheung, P. C. 1996. The hypocholesterolemic effect of two edible mushrooms: Auricularia auricula (tree-ear) and Tremella fuciformis (white jelly-leaf) in hypercholesterolemic $rats^1$. Nutr. Res. 16, 1721-1725. https://doi.org/10.1016/0271-5317(96)00191-1
  4. Cho, E. J., Hwang, H. J., Kim, S. W., Oh, J. Y., Baek, Y. M., Choi, J. W., Bae, S. H. and Yun, J. W. 2007. Hypoglycemic effects of exopolysaccharides produced by mycelial cultures of two different mushrooms Tremella fuciformis and Phellinus baumii in ob/ob mice. Appl. Microbiol. Biotechnol. 75, 1257-1265. https://doi.org/10.1007/s00253-007-0972-2
  5. Hanniffy, S., Wiedermann, U., Repa, A., Mercenier, A., Daniel, C., Fioramonti, J., Tlaskolova, H., Kozakova, H., Israelsen, H. and Madsen, S. 2004. Potential and opportunities for use of recombinant lactic acid bacteria in human health. Adv. Appl. Microbiol. 56, 1-64. https://doi.org/10.1016/S0065-2164(04)56001-X
  6. Hotamisligil, G. S., Peraldi, P., Budavari, A., Ellis, R., White, M. F. and Spiegelman, B. M. 1996. IRS-1-mediated inhibition of insulin receptor tyrosine kinase activity in TNF-${\alpha}$-and obesity-induced insulin resistance. Science 271, 665-670. https://doi.org/10.1126/science.271.5249.665
  7. Jung, H. K., Sim, M. O., Jang, J. H., Kim, T. M., An, B. K., Kim, M. S. and Jung, W. S. 2016. Anti-obesity effects of Peucedanum japonicum Thunberg L. on 3T3-L1 cells and high-fat diet-induced obese mice. Kor. J. Plant Res. 29, 1-10. https://doi.org/10.7732/kjpr.2016.29.1.001
  8. Kahn, B. B. and Flier, J. S. 2000. Obesity and insulin resistance. J. Clin. Invest. 106, 473-481. https://doi.org/10.1172/JCI10842
  9. Kahn, S. E., Hull, R. L. and Utzschneider, K. M. 2006. Mechanisms linking obesity to insulin resistance and type 2 diabetes. Nature 444, 840. https://doi.org/10.1038/nature05482
  10. Kalliomaki, M., Salminen, S., Arvilommi, H., Kero, P., Koskinen, P. and Isolauri, E. 2001. Probiotics in primary prevention of atopic disease: a randomised placebo-controlled trial. Lancet 357, 1076-1079. https://doi.org/10.1016/S0140-6736(00)04259-8
  11. Kim, T. H., Jo, S. H., Kim, M. J., Yu, Y. B., Jang, M. H. and Park, K. M. 2012. Comparative study on nutritional contents of Auricularia spp. J. Mushrooms 10, 29-36.
  12. Kim, Y. H., Han, K. I., Jeon, M., Hwang, S. G., Jung, E. G., Kwon, H. J. and Han, M. D. 2014. Antihyperlipidemic activities of a chemically engineered sulfated mushroom ${\beta}$-glucan on high fat dietary-induced hyperlipidemia in sprague-dawley rats. Life Sci. 24, 1209-1216. https://doi.org/10.5352/JLS.2014.24.11.1209
  13. Ko, M. S., Lee, S. J. and Kang, S. M. 2009. Effect of Tremella fuciformis Berk on anti-stress activities during long-term and short-term in mice. KSBB J. 24, 131-139.
  14. Lee, D. h., Park, J. S. and Lee, J. S. 2005. Quality and angiotensin I-converting enzyme inhibitor activity of korean traditional rice wine brewed by antihypertensive pholiota adiposa and S. cerevisiae. J. Mushrooms 3, 128-128.
  15. Lee, H. 2014. Methods for the assessment of insulin resistance. J. Kor. Diabetes 15, 7-11. https://doi.org/10.4093/jkd.2014.15.1.7
  16. Li, X., Wen, M. and Wang, K. 2011. Effect of Tremella fuciformis ferment substance on the growth performance and lipid metabolism of finishing pigs. J. Anim. Feed Sci. 20, 546-555. https://doi.org/10.22358/jafs/66211/2011
  17. Miyazawa, K., He, F., Yoda, K. and Hiramatsu, M. 2012. Potent effects of, and mechanisms for, modification of crosstalk between macrophages and adipocytes by lactobacilli. Curr. Top. Microbiol. Immunol. 56, 847-854. https://doi.org/10.1111/j.1348-0421.2012.00512.x
  18. Mokdad, A. H., Bowman, B. A., Ford, E. S., Vinicor, F., Marks, J. S. and Koplan, J. P. 2001. The continuing epidemics of obesity and diabetes in the united states. JAMA 286, 1195-1200. https://doi.org/10.1001/jama.286.10.1195
  19. Panduru, M., Panduru, N., Salavastru, C. and Tiplica, G. S. 2015. Probiotics and primary prevention of atopic dermatitis: a meta-analysis of randomized controlled studies. J. Eur. Acad. Dermatol. Venereol. 29, 232-242. https://doi.org/10.1111/jdv.12496
  20. Park, H. S. 2001. Pharmacological therapy of obesity. Kor. J. Obes. 10, 118-127.
  21. Park, M. J. 2005. Recent advances in regulating energy homeostasis and obesity. Kor. J. Pediatr. 48, 126-137.
  22. Perera, P. K. and Li, Y. 2011. Mushrooms as a functional food mediator in preventing and ameliorating diabetes. Funct. Food Health Dis. 1, 161-171. https://doi.org/10.31989/ffhd.v1i4.133
  23. Steppan, C. M. and Lazar, M. A. 2002. Resistin and obesity-associated insulin resistance. Trends Endocrinol. Metab. 13, 18-23. https://doi.org/10.1016/S1043-2760(01)00522-7
  24. Ukai, S., Hirose, K., Kiho, T. and Hara, C. 1974. Polysaccharides in Fungi. I. Purification and characterization of acidic heteroglycans from aqueous extract of Tremella Fuciformis Berk. Chem. Pharm. Bull. 22, 1102-1107. https://doi.org/10.1248/cpb.22.1102
  25. Ukai, S., Hirose, K., Kiho, T., Hara, C., Irikura, T., Kanechika, T. and Hasegawa, Y. 1972. Antitumor activity on sarcoma 180 of the polysaccharides from Tremella fuciformis Berk. Chem. Pharm. Bull. 20, 2293-2294. https://doi.org/10.1248/cpb.20.2293
  26. Woo, S. E., Kwon, J. H., Yang, S. Y., Park, H. J. and Kim, K. 2008. Development of egg yolk antibody specific to the pancreatic lipase domain for anti-obesity. Kor. J. Microbiol. Biotechnol. 36, 299-306.
  27. Wu, Y. J., Wei, Z. X., Zhang, F. M., Linhardt, R. J., Sun, P. L. and Zhang, A. Q. 2018. Structure, bioactivities and applications of the polysaccharides from Tremella fuciformis mushroom: A review. Int. J. Biol. Macromol. 121, 1005-1010. https://doi.org/10.1016/j.ijbiomac.2018.10.117
  28. Yang, J. O. 2014. The current status and prospect of probiotic research and industrialization. Food Sci. Ind. 47, 29-44.