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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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MiR-371 promotes proliferation and metastasis in hepatocellular carcinoma by targeting PTEN

  • Wang, Hao (Eastern Hepatobiliary Surgery Hospital (EHBH), Second Military Medical University) ;
  • Zhao, Yi (Eastern Hepatobiliary Surgery Hospital (EHBH), Second Military Medical University) ;
  • Chen, Tingsong (The Seventh People's Hospital of Shanghai) ;
  • Liu, Guofang (Eastern Hepatobiliary Surgery Hospital (EHBH), Second Military Medical University) ;
  • He, Nan (Guangdong Ascendas Genomics Technology Co., Ltd.) ;
  • Hu, Heping (Eastern Hepatobiliary Surgery Hospital (EHBH), Second Military Medical University)
  • Received : 2018.07.19
  • Accepted : 2018.09.19
  • Published : 2019.05.31
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Abstract

Hepatocellular carcinoma (HCC) is the leading cause of cancer-related mortality worldwide. MiR-371 has recently emerged as an important regulator in tumorigenesis, and may serve as a biomarker for malignant tumors. We transfected miR-371 or its inhibitor in two human HCC cell lines, then used 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, soft agar colony formation, and transwell migration assays to evaluate the effects on cell proliferation, migration, and invasion. We found that miR-371 was positively correlated with HCC metastasis and poor prognosis in the inflicted patients, and the high expression of miR-371 was promoted, whereas a low level of miR-371 depressed cell proliferation and invasion. We found PTEN to be a direct target of miR-371. The overexpression or knockdown of PTEN exhibited the opposite effects from those of miR-371 on cell proliferation and migration. Our study demonstrates that miR-371 promotes proliferation and metastasis in HCC by targeting PTEN.

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References

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