Antimicrobial Effects of Tea Tree (Melaleuca alternifolia) Extracts

티트리 (Melaleuca alternifolia) 추출물의 항균 활성

  • Ji, Keunho (Basic Science Research Institute, Pukyong National University)
  • 지근호 (부경대학교 기초과학연구소)
  • Received : 2019.02.25
  • Accepted : 2019.05.03
  • Published : 2019.05.31


Melaleuca alternifolia contains terpineol-4, cineol, sesquiterpenes etc., and has a germicidal effect and skin moisturizing effect. It also has the characteristics of relieving acne inflammation, treating dandruff, relieving pain, and relieving depression. In this study, an antimicrobial substance extracted from tea tree using an organic solvent (methanol, dichloromethane, ethyl acetate) and hydrothermal extraction method. And confirmed the antimicrobial activity of each extract. In order to verify the antimicrobial activity, nine pathogenic bacteria (Bacillus cereus, Bacillus subtilis, Listeria monocytogenes, Enterococcus faecalis, Staphylococcus aureus, Klebsiella pneumoniae, Escherichia coli, Salmonella typhimurium, Pseudomonas aeruginosa and Vibrio parahaemolyticus) were used. The antimicrobial activity of each extracts were confirmed by the commonly used disc diffusion method. The results showed that the fraction extracts of ethyl acetate and methanol had antimicrobial effects against V. parahaemolyticus and S. aureus. Using these results, we confirmed the antimicrobial activity of each fraction extracts and hot water extracts against V. parahaemolyticus. After the treat of samples, we confirmed at over 99.9 % of antimicrobial activity. In case of antifungal activities, we confirmed of preservation effect during over 45 hours. Based on the results of this research, further studies will be conducted to confirm the possibility of development as a new antimicrobial agent.

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Fig. 1. The procedure for each extraction from Melaleuca alternifolia.

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Fig. 2. The antimicrobial activities of M. alternifolia extracts. C; DMSO control, D; dichloromethane extract, E; ethyl acetate extract, H; water layer

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Fig. 3. The antimicrobial activities of M. alternifolia extracts. B; MeOH control, 1; 99 % MeOH extract, 2; 70 % MeOH extract

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Fig. 4. The antimicrobial activities of M. alternifolia extracts. B; DMSO control, 1; MeOHext- DMSOdis, 2; MeOHext-H2Odis, 3; Hot water extract

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Fig. 5. The antifungal activities of M. alternifolia extracts against A. niger . A; 24hr, B; 45hr, C; 70hr, D; 95hr, E; 145hr, (1); Control, (2); 70 % MeOH extract, (3); 99 % MeOH extract, (4); MeOHext-DMSOdis, (5); Hot water extract

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Fig. 6. The antifungal activities of M. alternifolia extracts against C . globosum . A; 24hr, B; 45hr, C; 70hr, D; 95hr, E; 145hr, (1); Control, (2); 70 % MeOH extract, (3); 99 % MeOH extract, (4); MeOHext-DMSOdis, (5); Hot water extract

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Fig. 7. The antifungal activities of M. alternifolia extracts against P. pinophilum . A; 24hr, B; 45hr, C; 70hr, D; 95hr, E; 145hr, (1); Control, (2); 70 % MeOH extract, (3); 99 % MeOH extract, (4); MeOHext-DMSOdis, (5); Hot water extract

Table 1. Growth conditions of pathogenic bacteria

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Table 2. Antimicrobial effects of M. alternifolia various extracts against V. parahaemolyticus.

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Supported by : 한국연구재단


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