Asian-Australasian Journal of Animal Sciences

  • 제32권7호
  • /
  • Pages.996-1006
  • /
  • 2019
  • /
  • 1011-2367(pISSN)
  • /
  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
권호 표지
DOI QR코드

DOI QR Code

Dynamics of fungal community during silage fermentation of elephant grass (Pennisetum purpureum) produced in northern Vietnam

  • Vu, Viet Ha (Key Laboratory of Bio-resource and Bio-control of the Ministry of Education, College of Life Sciences, Sichuan University) ;
  • Li, Xiyang (Key Laboratory of Bio-resource and Bio-control of the Ministry of Education, College of Life Sciences, Sichuan University) ;
  • Wang, Mengyuan (Key Laboratory of Bio-resource and Bio-control of the Ministry of Education, College of Life Sciences, Sichuan University) ;
  • Liu, Rongmei (Key Laboratory of Bio-resource and Bio-control of the Ministry of Education, College of Life Sciences, Sichuan University) ;
  • Zhang, Guojian (Key Laboratory of Bio-resource and Bio-control of the Ministry of Education, College of Life Sciences, Sichuan University) ;
  • Liu, Wei (Key Laboratory of Bio-resource and Bio-control of the Ministry of Education, College of Life Sciences, Sichuan University) ;
  • Xia, Baixue (Key Laboratory of Bio-resource and Bio-control of the Ministry of Education, College of Life Sciences, Sichuan University) ;
  • Sun, Qun (Key Laboratory of Bio-resource and Bio-control of the Ministry of Education, College of Life Sciences, Sichuan University)
  • 투고 : 2018.09.14
  • 심사 : 2019.01.08
  • 발행 : 2019.07.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objective: This study aimed to gain deeper insights into the dynamic changes in spoilage fungi populations during fermentation and the influence of traditional additives on silage quality. Methods: Elephant grass (Pennisetum purpureum) was prepared without any additive (control), and with the addition of 0.5% salt, and 0.5% salt-0.2% sugar mixture. The fungal community was then determined using a classic culturing method and high-throughput sequencing at 0, 5, 15, and 60 days after ensiling. Results: The results showed that the fungal community of elephant grass silage varied significantly between the natural fermentation without any additive and the two additive groups. The diversity and relative abundance of spoilage molds in the control group were much higher than those in the two treatment groups (p<0.05). Three species of yeasts (Candida sp., Pichia sp., Trichosporon sp.) and four spoilage molds (Fusarium sp., Aspergillus sp., Muco sp. and Penicillin sp.) were the predominant fungi in elephant grass during natural fermentation from 0 to 60 days, which were found to be significantly decreased in salt and sugar additive groups (p<0.05). Meanwhile, the diversity and relative abundance of undesirable molds in the 0.5%-salt additive group were the lowest among all groups. Conclusion: Adding salt and sugar, particularly 0.5% salt, is a promising effective approach to reduce the amount of undesirable fungi thus, improving the silage quality of elephant grass in northern Vietnam.

키워드

참고문헌

  1. Tran TMT, Nguyen MT, Nguyen HV, Nishino N. Effects of wilting and lactic acid bacteria inoculation on fermentation and microbial community of elephant grass silage produced in Vietnam. Grassl Sci 2018;64:151-5. https://doi.org/10.1111/grs.12187
  2. Ranjit NK, Kung Jr L. The effect of Lactobacillus buchneri, Lactobacillus plantarum, or a chemical preservative on the fermentation and aerobic stability of corn silage. J Dairy Sci 2000;83:526-35. https://doi.org/10.3168/jds.S0022-0302(00)74912-5
  3. Sun ZH, Liu SM, Tayo GO, et al. Effects of cellulase or lactic acid bacteria on silage fermentation and in vitro gas production of several morphological fractions of maize stover. Anim Feed Sci Technol 2009;152:219-31. https://doi.org/10.1016/j.anifeedsci.2009.04.013
  4. O'Brien M, Egan D, O'Kiely P. Morphological and molecular characterisation of Penicillium roqueforti and P. paneum isolated from baled grass silage. Mycol Res 2008;112:921-32. https://doi.org/10.1016/j.mycres.2008.01.023
  5. Kasmaei KM, Rustas BO, Sporndly R, Uden P. Prediction models of silage fermentation products on crop composition under strict anaerobic conditions: a meta-analysis. J Dairy Sci 2013;96:6644-9. https://doi.org/10.3168/jds.2013-6858
  6. Duniere L, Sindou J, Chaucheyrasdurand F, Chevallier I, Thevenotsergentet D. Silage processing and strategies to prevent persistence of undesirable microorganisms. Anim Feed Sci Technol 2013;182:1-15. https://doi.org/10.1016/j.anifeedsci.2013.04.006
  7. Danner H, Holzer M, Mayrhuber E, Braun R. Acetic acid increases stability of silage under aerobic conditions. Appl Environ Microbiol 2003;69:562-7. https://dx.doi.org/10.1128%2FAEM.69.1.562-567.2003 https://doi.org/10.1128/AEM.69.1.562-567.2003
  8. Lowes KF, Shearman CA, Payne J, et al. Prevention of yeast spoilage in feed and food by the yeast mycocin HMK. Appl Environ Microbiol 2000;66:1066-76. https://dx.doi.org/10.1128/AEM.66.3.1066-1076.2000
  9. Roige MB, Aranguren SM, Riccio MB, Pereyra S, Soraci AL, Tapia MO. Mycobiota and mycotoxins in fermented feed, wheat grains and corn grains in Southeastern Buenos Aires Province, Argentina. Rev Iberoam Micol 2009;26:233-7. https://doi.org/10.1016/j.riam.2009.03.003
  10. Morgavi DP, Riley RT. An historical overview of field disease outbreaks known or suspected to be caused by consumption of feeds contaminated with Fusarium toxins. Anim Feed Sci Technol 2007;137:201-12. https://doi.org/10.1016/j.anifeedsci.2007.06.002
  11. Herrmann C, Heiermann M, Idler C. Effects of ensiling, silage additives and storage period on methane formation of biogas crops. Bioresour Technol 2011;102:5153-61. https://doi.org/10.1016/j.biortech.2011.01.012
  12. Chai L, Li HJ, Zhang JF, et al. Expression of aquaporin BnPIP-like from rapeseed (Brassica napus L.) enhances salt resistance in yeast (Pichia pastoris). Int J Agric Biol 2016;18:1256-62. https://doi.org/10.17957/IJAB/15.0240
  13. Aksu T, Baytok E, Karsli MA, Muruz H. Effects of formic acid, molasses and inoculant additives on corn silage composition, organic matter digestibility and microbial protein synthesis in sheep. Small Rumin Res 2006;61:29-33. https://doi.org/10.1016/j.smallrumres.2004.12.013
  14. Pitt JL, Hocking AD. Fungi and food spoilage. New York, USA: Chapman Hall; 1997.
  15. Bolsen KK, Ashbell G, Weinberg ZG. Silage fermentation and silage additives - review. Asian-Australas J Anim Sci 1996;9:483-93. https://doi.org/10.5713/ajas.1996.483
  16. McLaughlin AM, Erickson PS, Hussey JS, Reid ED, Tanguay JA. NaCl addition to the top layer of corn ensiled in bunker silos. Prof Anim Sci 2002;18:90-2. https://doi.org/10.15232/S1080-7446(15)31490-X
  17. Zheng ML, Niu DZ, Jiang D, Zuo SS, Xu CC. Dynamics of microbial community during ensiling direct-cut alfalfa with and without LAB inoculant and sugar. J Appl Microbiol 2017;122:1456-70. https://doi.org/10.1111/jam.13456
  18. Grazia L, Suzzi G, Romano P. Isolation and identification of moulds in maize silage. Inf Agrar 1990;46:57-9.
  19. Santos MC, Golt C, Joerger RD, Mechor GD, Mourao GB, Kung Jr L. Identification of the major yeasts isolated from high moisture corn and corn silages in the United States using genetic and biochemical methods. J Dairy Sci 2017;100:1151-60. https://doi.org/10.3168/jds.2016-11450
  20. Pedroso ADF, Nussio LG, Paziani SDF, et al. Fermentation and epiphytic microflora dynamics in sugar cane silage. Sci Agric 2005;62:427-32. http://dx.doi.org/10.1590/S0103-90162005000500003
  21. Driehuis F, Elferink SJWHO, Spoelstra SF. Anaerobic lactic acid degradation during ensilage of whole crop maize inoculated with Lactobacillus buchneri inhibits yeast growth and improves aerobic stability. J Appl Microbiol 1999;87:583-94. https://doi.org/10.1046/j.1365-2672.1999.00856.x
  22. Orsi RB, Correa B, Possi CR, et al. Mycoflora and occurrence of fumonisins in freshly harvested and stored hybrid maize. J Stored Prod Res 2000;36:75-87. https://doi.org/10.1016/S0022-474X(99)00029-6
  23. Panagou EZ, Skandamis PN, Nychas GJE. Modelling the combined effect of temperature, pH and aw on the growth rate of Monascus ruber, a heat-resistant fungus isolated from green table olives. J Appl Microbiol 2003;94:146-56. https://doi.org/10.1046/j.1365-2672.2003.01818.x
  24. Merry RJ, Davies DR. Propionibacteria and their role in the biological control of aerobic spoilage in silage. Dairy Sci Technol 1999;79:149-64. https://doi.org/10.1051/lait:1999112
  25. Zheng ZH, Liu JH, Yuan XF, et al. Effect of dairy manure to switchgrass co-digestion ratio on methane production and the bacterial community in batch anaerobic digestion. Appl Energy 2015;151:249-57. https://doi.org/10.1016/j.apenergy.2015.04.078
  26. Myllykoski J, Lindstrom M, Ketotimonen R, et al. Type C bovine botulism outbreak due to carcass contaminated non-acidified silage. Epidemiol Infect 2009;137:284-93. https://doi.org/10.1017/S0950268808000939
  27. Liu J, Kondo S, Sekine J, Okubo M, Asahida Y. The nutritive values of grass, corn and rice silage feed to sheep at diffrent levels. J Fac Agric Hokkaido University 1986;63:125-35.
  28. Knicky M, Sporndly R. The ensiling capability of a mixture of sodium benzoate, potassium sorbate, and sodium nitrite. J Dairy Sci 2011;94:824-31. https://doi.org/10.3168/jds.2010-3364
  29. Aschenbach JR, Gabel G. Effect and absorption of histamine in sheep rumen: significance of acidotic epithelial damage. J Anim Sci 2000;78:464-70. https://doi.org/10.2527/2000.782464x
  30. GMP. Certification Scheme Animal Feed Sector, 2006.Version Marzo 2008. Appendix 1: product standards (including residue standards). The Hague: Productschap Diervoeder. 1-39.

피인용 문헌

  1. Effect of using inoculant on elephant grass silage with additives vol.42, 2020, https://doi.org/10.4025/actascianimsci.v42i1.50533
  2. Yeast population dynamics on air exposure in total mixed ration silage with sweet potato residue vol.91, pp.1, 2020, https://doi.org/10.1111/asj.13397
  3. Characterisation of fungal contamination sources for use in quality management of cheese production farms in Korea vol.33, pp.6, 2019, https://doi.org/10.5713/ajas.19.0553
  4. Using molecular microbial ecology to define differential responses to the inoculation of barley silage vol.100, pp.4, 2019, https://doi.org/10.1139/cjas-2019-0214