대한임상검사과학회지 (Korean Journal of Clinical Laboratory Science)

  • 제51권2호
  • /
  • Pages.177-184
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  • 2019
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  • 1738-3544(pISSN)
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  • 2288-1662(eISSN)
대한임상검사과학회 (Korean Society for Clinical Laboratory Science)
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마이코박테리움을 신속하게 배양할 수 있는 성장 인자에 관한 연구

Study on the Growth Factors for Rapidly Cultivating Mycobacterium spp.

  • 하성일 (가톨릭대학교 서울성모병원 진단검사의학과) ;
  • 박강균 (가톨릭대학교 서울성모병원 진단검사의학과) ;
  • 석현수 (가톨릭대학교 서울성모병원 진단검사의학과) ;
  • 신정섭 (가톨릭대학교 서울성모병원 진단검사의학과) ;
  • 신동필 (가톨릭대학교 서울성모병원 진단검사의학과) ;
  • 권민오 (가톨릭대학교 서울성모병원 진단검사의학과) ;
  • 박연준 (가톨릭대학교 의과대학 진단검사의학과)
  • Ha, Sung-Il (Department of Laboratory Medicine, The Catholic University of Korea, Seoul Saint Mary's Hospital) ;
  • Park, Kang-Gyun (Department of Laboratory Medicine, The Catholic University of Korea, Seoul Saint Mary's Hospital) ;
  • Suk, Hyun-Soo (Department of Laboratory Medicine, The Catholic University of Korea, Seoul Saint Mary's Hospital) ;
  • Shin, Jeong-Seob (Department of Laboratory Medicine, The Catholic University of Korea, Seoul Saint Mary's Hospital) ;
  • Shin, Dong-Pil (Department of Laboratory Medicine, The Catholic University of Korea, Seoul Saint Mary's Hospital) ;
  • Kwon, Min-O (Department of Laboratory Medicine, The Catholic University of Korea, Seoul Saint Mary's Hospital) ;
  • Park, Yeon-Joon (Department of Laboratory Medicine, The Catholic University of Korea, College of Medicine)
  • 투고 : 2019.02.01
  • 심사 : 2019.03.12
  • 발행 : 2019.06.30
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초록

Mycobacterium은 느리게 성장한다. 따라서 고체배지는 8주, 액체배지는 6주 동안 사용하여야 한다. 이 연구의 목적은 Mycobacterium을 빠르게 성장시킬 수 있는 성장 인자를 찾고, 신속한 동정을 위한 고체배지를 개발하는 데 도움을 주는 것이다. $Difco^{TM}$ Mycobacteria 7H11 agar (Becton, Dickinson and Company)에 activated charcoal, defibrinated sheep blood, L-ascorbic acid를 첨가하여 10종의 Mycobacteria 가지고 Mycobacterium 성장 인자 3가지를 평가하였다. 집락의 검출 시간 및 판독 용이성을 현재의 방법과 비교하였다. 빠르게 성장하는 Mycobacterium 있어 새로운 배지와 기존의 배지에서 검출 시간의 차이는 새로운 배지가 더 빠르다는 것을 확인시켜 주었다. M. kansasii와 M. intracelluare는 7H11 배지보다 7H11 C 배지에서 더 빠르게 자라는 것으로 확인되었다. MTB는 7H11 C 배지에서 다른 배지보다 빠르게 성장하였다. 이 연구는 2 두 가지 성장 인자가 빠르게 성장하는 Mycobacteria과 느리게 성장하는 Mycobacteria에 영향을 주는 것으로 확인되었다. 7H11 C 배지는 색상 대비로 인하여 10종의 모든 Mycobacterium에서 기존배지보다 더 뛰어난 판독 용이성을 보여 주었다. 특히, MTB가 성장했을 경우 집락의 크기가 다른 배지에서 보다 커서 시각화가 용이하였다.

Mycobacteria grow slowly. Therefore, a solid medium should be used for eight weeks and a liquid medium for six weeks. The purpose of this study was to find the growth factors that can grow Mycobacterium rapidly and to help develop a solid medium for rapid identification. Three types of Mycobacterium growth factors were evaluated with 10 Mycobacteria by adding activated charcoal, defibrinated sheep blood, and L-ascorbic acid to $Difco^{TM}$ Mycobacteria 7H11 agar (Becton, Dickinson and Company, Sparks, MD, USA). The time to detection and the distinguishability of a colony were compared with that of the current method. In the rapidly growing Mycobacterium, the difference in detection time between the new media and conventional media confirmed that the new media was faster. M. kansasii and M. intracelluare grew faster in 7H11 C than in 7H11 medium. MTB grew faster than the other media in 7H11 C. This study confirmed that the two growth factors affect fast-growing Mycobacteria and slow-growing Mycobacteria. 7H11 C showed better distinguishability than the conventional media in all 10 Mycobacterium due to the color contrast. In particular, when the MTB was grown, the size of the colonies was larger than with other media, so visualization was easy.

키워드

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