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Antioxidant activity and improvement effect of Acer tegmentosum Maxim of dietary fatty liver in rat fed on a high-fat diet

벌나무(Acer tegmentosum Maxim) 추출물의 항산화 활성 및 고지방식이를 급이한 흰쥐의 지방간 개선 효과

  • Lee, Soo-Jung (Institute of Agriculture and Life Sciences, Gyeongsang National University) ;
  • Cho, Hang Hee (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
  • Song, Yuno (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
  • Jang, Sun-Hee (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
  • Cho, Jae-Hyeon (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
  • 이수정 (경상대학교 농업생명과학연구원) ;
  • 조항희 (경상대학교 수의학과 동물의학연구원) ;
  • 송윤오 (경상대학교 수의학과 동물의학연구원) ;
  • 장선희 (경상대학교 수의학과 동물의학연구원) ;
  • 조재현 (경상대학교 수의학과 동물의학연구원)
  • Received : 2018.12.04
  • Accepted : 2019.03.11
  • Published : 2019.06.30

Abstract

The effects on the radical scavenging activities and hepatic lipid levels in rats fed a high-fat diet (HFD) in the 70% ethanolic extract from Acer tegmentosum Maxim (ATM) were evaluated. Total phenol content of ATM was 168.60 mg catechin/g in the 70% ethanolic extract of Acer tegmentosum. The DPPH and ABTS radical scavenging activities were 18.32 mM TE/g and 32.25 mM TE/g, respectively. Food efficiency ratio was lower significantly in supplemented group with 150 mg/kg BW/day during 5 weeks (HFD+ATM) compared to HFD. Total cholesterol and triglyceride contents in liver tissue of HFD+ATM were lower significantly compared to those of the HFD. Supplementation of ATM significantly decreased lipid peroxide contents and increased radical scavenging activity in the liver tissue compared with that of HFD group. Moreover, the hepatocytes of HFD rats showed a typical fatty liver morphology showing the presence of cytoplasmic lipid droplets, whereas administration of ATM attenuated the number and the size of lipid droplets. In the liver tissue of ATM administrated HFD group, the mRNA levels of SREBP-1c, $PPAR{\gamma}$, and FAS were decreased. Therefore, these results suggest that Acer tegmentosum extracts could have antioxidant activities and the hypolipidemic effects in liver tissue by its phenolic compounds.

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Fig. 1. Effect of Acer tegmentosum extract for the ALT and ASTactivities in serum of rats fed ahigh-fat diet. Different letters insuperscript following values indicate statistical significance (P<0.05) as determined by Duncan'smultiple range test.

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Fig. 2. Effect of Acer tegmentosum extract for the total cholesteroland triglyceride level in liver tissues of rats fed a high-fat diet. Different letters in superscript fol-lowing values indicate statistical significance (P<0.05) as determinedby Duncan's multiple range test.

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Fig. 3. Representative photographs showing liver tissue histology in rats fed a high-fat diet.

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Fig. 4. Effect of ATM on hepatic mRNA expresssion of lipogenesisrelated genes in high fat diet-fed rats. Optical density analysis was performed to quantify the levels of mRNA expression with GAPDH as loading control. Different letters in superscript following values indicate statistical significance (P< 0.05) as determined by Duncan's multiple range test.

Table 1. Radical scavenging activities, total phenol and flavonoids contents in 70% ethanol extract from Acer tegmentosum

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Table 2. Effect of Acer tegmentosum extract for food intake, body weight gain and ood efficiency ratio in rats fed a high-fat diet

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Table 3. Effect of Acer tegmentosum extract on lipid peroxide contents and DPPH radical scavenging activity in liver tissue of rats fed a high-fat diet

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Acknowledgement

Supported by : 한국연구재단

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