DOI QR코드

DOI QR Code

A Study on Single-dose Toxicity and Repeated-Dose Toxicity of Drosera Rotundifolia L. Pharmacopuncture in Rodent Models

원엽모고채 약침의 설치류에 대한 단회 및 반복 독성연구

  • Lee, Yong Eun (Department of Acupuncture, Moxibustion and Acupoint, College of Korean Medicine, Daegu Haany University) ;
  • Lee, Jung Hee (Department of Acupuncture, Moxibustion and Acupoint, College of Korean Medicine, Daegu Haany University) ;
  • Lee, Yun Kyu (Department of Acupuncture, Moxibustion and Acupoint, College of Korean Medicine, Daegu Haany University) ;
  • Lee, Bong Hyo (Department of Acupuncture, Moxibustion and Acupoint, College of Korean Medicine, Daegu Haany University) ;
  • Kim, Jae Soo (Department of Acupuncture, Moxibustion and Acupoint, College of Korean Medicine, Daegu Haany University) ;
  • Lee, Hyun-Jong (Department of Acupuncture, Moxibustion and Acupoint, College of Korean Medicine, Daegu Haany University)
  • 이용은 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 이정희 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 이윤규 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 이봉효 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 김재수 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 이현종 (대구한의대학교 한의과대학 침구경혈학교실)
  • Received : 2019.05.17
  • Accepted : 2019.06.17
  • Published : 2019.06.27

Abstract

Objectives : This study was carried out in order to toxicity and safety of Drosera Rotundifolia L. pharmacopuncture in BALB/c mice. Methods : In order to investigate toxicity and safety of Drosera Rotundifolia L. pharmacopuncture, BALB/c mice were chosen in these studies and injected to ST36. In 50% Lethal Dose (LD 50) study, mice were divided into 8 groups. Doses of pharmacopuncture at 300, 600, 1,200, 2,400, 3,600, 4,800, 6,000, 7,200 mg/kg were given to the each group. we observed mortality and toxic signs for 7 days after injection. In single-dose toxicity, mice were divided into 4 groups. Doses of pharmacopuncture, 300, 600, 1,200 mg/kg, were administered to the experimental group. And a dose of normal saline, 0.2 ml was administered to the control group. We observed mortality and toxic signs for 14 days after injection. In repeated-dose toxicity for 4 weeks, mice were divided in 4 groups. Doses of pharmacopuncture, 150, 300, 600 mg/kg, were administered to the experimental group. And a dose of normal saline, 0.2 ml, was administered to the control group. We observed mortality, toxic signs, body weight, hematological values et al. Results : The LD 50 of Drosera Rotundifolia L. pharmacopuncture was 4,800 mg/kg. In single-dose toxicity, no deaths, no toxic signs occurred in any of groups. In repeated-dose toxicity for 4 weeks, no deaths, no toxic signs occurred in any of groups. Conclusions : No Observed Adverse Effect Level (NOAEL) of Drosera Rotundifolia L. pharmacopuncture injection for 4 weeks was more than 600 mg/kg.

References

  1. Korean Acupuncture & Moxibustion Society Textbook Compilation Committee. The Acupuncture and Moxibustion Medicine. 2nd ed. Gyeonggi:Jipmoondang. 2012: 230-1.
  2. Sin DC, Song HS. Cuttent Situation of Translational Research on Acupunctue, Moxibustion and Pharmacopuncture. Journal of Acupuncture Research. 2016; 33(2): 109-16. https://doi.org/10.13045/acupunct.2016021 https://doi.org/10.13045/acupunct.2016021
  3. Ministry of Food & Drug Safety. A Flow Chart on Toxicity Test of Medicine(2015)[Internet]. Cheongwon:Ministry of Food & Drug Safety. [citied 2015 November 11]. available from: URL:https://www.mfds.go.kr/brd/m_210/view.do?seq=12245
  4. Kam HM, Lim CS, Kwon KR, Lee KH. Study of A 13-weeks, Repeated, Intramuscular Dose, Toxicity Test of Sweet Bee Venom in Sprague-Dawley Rats. Journal of Pharmacopuncture. 2014; 17(2): 73-9. https://doi.org/10.3831/kpi.2014.17.019 https://doi.org/10.3831/KPI.2014.17.019
  5. Lee HY, Kim SC, Cho SH. Assessment of The Toxicity and The Stability of Saeng Mak San by Using Repeated Intravenous Injections in Sprague-Dawley Rats. Journal of Pharmacopuncture. 2016; 19(3): 231-8. DOI : 10.3831/KPI.2016.19.024. https://doi.org/10.3831/KPI.2016.19.024
  6. Lee JH, Ha IH, Kim MR, Kim MJ, Lee JW, Lee IH, et al. Multiple-dose intramuscular Toxiticy Study of Shinbaro Pharmacopuncture in Sprague-Dawley Rats Over A 4-week Period. Journal of Acupuncture Research. 2015; 32(4): 77-89. https://doi.org/10.13045/acupunct.2015063 https://doi.org/10.13045/acupunct.2015063
  7. Lim SC, Kim JS, Lee BH, Lee HJ, Lee H, Lee YK. Thirteen Weeks Repeated-dose Toxicity Study on Aconitum ciliare Decaisne Pharmacopuncture Solution in Mice. Korean Journal of Acupuncture. 2018; 35(3): 139-48. https://doi.org/10.14406/acu.2018.022 https://doi.org/10.14406/acu.2018.022
  8. Lee HS, Lee YK, Lee BH, Kim JS, Lim SC, Lee HJ. An Experimental Study on Single-dose and 4 Weeks Repeated-dose Toxicity of Aconitum ciliare Decaisne Pharmacopuncture. Korean Journal of Acupuncture. 2017; 34(4): 241-50. https://doi.org/10.14406/acu.2017.027 https://doi.org/10.14406/acu.2017.027
  9. Yu JS, Sun SS, Lee KW, Kwon KR. Intravenous Toxicity Study of Water-soluble Ginseng Pharmacopuncture in SD rats. Journal of Pharmacopuncutre. 2015; 18(4): 38-44. https://doi.org/10.3831/kpi.2015.18.036 https://doi.org/10.3831/KPI.2015.18.036
  10. Lee MS, Jung C. Single Intramuscular Dose Toxicity Study of CA-YAKCHIM in Sprague-Dawley Rats. Journal of Korea Immuno-yakchim Society. 2014; 3(1): 19-26.
  11. Lee SJ, Jeong HH, Lee JC, Cha EH, Park MY, Song BG, et al. A Study on Single Dose Toxicity of Intravenous Injection of Mecasin Herbal Acupuncture. Journal of Acupuncture Research. 2016; 33(1): 1-7. https://doi.org/10.13045/acupunct.2016001 https://doi.org/10.13045/acupunct.2016001
  12. Son IH, Lee SM, Kim DH, Jeong HH, Cho SH, Lee EY, et al. A Pilot Study on Single-dose Toxicity Testing of Scolopendrid Pharmacopuncture in Sprague-Dawley Rats. Journal of Pharmacopuncture. 2014; 17(2): 57-66. https://doi.org/10.3831/kpi.2014.17.017 https://doi.org/10.3831/KPI.2014.17.017
  13. Lee HG, Kim SC, Jung DJ, Choi YM, Sin MS, Choi SW et al. Single Intramuscular-dose Toxicity of Water Soluble Carthmi-Flos Herbal Acupuncture(WCF) in Sprague-Dawley Rats. Journal of Pharmacopuncture. 2014; 17(1): 27-34. https://doi.org/10.3831/kpi.2014.17.004 https://doi.org/10.3831/KPI.2014.17.004
  14. Moon GS. Ingredient and Use of Medicinal Herb. Seoul:Ilweoseogak. 1996: 321-2.
  15. The Compilation Committee. Chinese Medicinal Herb Pictorial Book. Seoul : Yeogang. 1994:1154.
  16. Fukushima K, Nagai K, Hoshi Y, Masumoto S, Mikami I, Takahashi Y, et al. Drosera Rotundifolia And Drosera Tokaiensis Supress The Activation of HMC-1 Human Mast Cells. Journal of Ethnopharmacology. 2009; 125(1): 90-6. https://doi.org/10.1016/j.jep.2009.06.009 https://doi.org/10.1016/j.jep.2009.06.009
  17. Melzig M.F, Pertz H.H, Krenn L. Anti-inflammatory And Spasmolytic Activity of Extracts From Droserae Herba. Phytomedicine. 2001; 8(3): 225-9. https://doi.org/10.1078/0944-7113-00031 https://doi.org/10.1078/0944-7113-00031
  18. Oh SJ, Kim JS, Lee YK, Lee HJ. Analgesic Effects of Drosera Rotundifolia L. Pharmacopuncture at Taegye(KI3) Acupoint on Formalin-induced Pain. Journal of Acupuncture Research. 2016; 33(1): 37-46. https://doi.org/10.13045/acupunct.2016004 https://doi.org/10.13045/acupunct.2016004
  19. Koo ST, Kim SK, Kim EH, Kim JH, Youn DH, Lee BH, et al. Acupuncture Point Locations for Experimental Animal Studies in Rats and Mice. Journal of Acupuncture Reserach. 2010; 27(3): 67-78.
  20. Lee H, Cha SS, Lee MY, Chang HC, Lee JJ. Acute Toxicity of Leuconostoc Citreum GR1 Isolated from Kimchi in Mice. Korean Journal of Food Preservation. 2013; 20(1): 121-6. https://doi.org/10.11002/kjfp.2013.20.1.121 https://doi.org/10.11002/kjfp.2013.20.1.121
  21. Vaghasiya Y.K., Shukla V.J., Chanda S.V. Acute Oral Toxicity Study of Pluchea arguta Boiss Extract in Mice. Journal of Pharmacology & Toxicology. 2011; 6(2): 113-3. https://doi.org/10.3923/jpt.2011.113.123 https://doi.org/10.3923/jpt.2011.113.123
  22. Kang JH, Heo DS, Yoon IJ, Oh MS. An Analysis of Tendencies of Studies on Herbal Acupuncture-Focusing on Domestic Theses Since 2001 About Anti-inflammation, Pain Relief And Anti-obesity Effects, Including Safety. The Journal of Korean Medicine. 2007; 28(2): 93-113.
  23. Sin DC, Song HS. Cuttent Situation of Translational Research on Acupunctue, Moxibustion and Pharmacopuncture. Journal of Acupuncture Research. 2016; 33(2): 109-16. https://doi.org/10.13045/acupunct.2016021 https://doi.org/10.13045/acupunct.2016021
  24. Ren YS, Zhang P, Yan D, Wang JB, Du XX, Xiao XH. A Strategy for The Dectiontion of Quality Fluctuation of A Chinese Herbal Injection Based on Chemical Fingerprinting Combined with Biological Fingerprinting. Journal of Pharmaceutical and Biomedical Analysis. 2011; 56(2): 436-42. https://doi.org/10.1016/j.jpba.2011.05.006 https://doi.org/10.1016/j.jpba.2011.05.006
  25. Joo HJ. Researches on Pharmacopuncture. Korea Institute of Oriental medicine. 1995; 5: 193-210.
  26. Lee SJ, Jeong HH, Lee JC, Cha EH, Park MY, Song BG, et al. A Study on Single Dose Toxicity of Intravenous Injection of Mecasin Herbal Acupuncture. Journal of Acupuncture Research. 2016; 33(1): 1-7. https://doi.org/10.13045/acupunct.2016001 https://doi.org/10.13045/acupunct.2016001
  27. Lee JH, Chung HJ, Lee IH, Lee JW, Kim EJ, Kim MJ. Study on Single-dose Intramuscular Toxicity of Shinbaro Pharmacopuncture in Sprague-Dawley Rats and Beagle Dogs. Journal of Korean Medicine Rehabilitation. 2015; 25(3): 1-9. https://doi.org/10.18325/jkmr.2015.25.3.1 https://doi.org/10.18325/jkmr.2015.25.3.1
  28. Baek SH, Lee IH, Kim MJ, Kim EJ, Ha IH, Lee JH, et al. Component Analysis and Toxicity Study of Combined Cultuvated Wild Genseng Pharmacopuncture. The Journal of Internal Korean Medcine. 2015; 36(2): 189-99.
  29. Park YC, Cho MH. A New Way in Deciding NOAEL Based on The Findings From GLP-Toxicity Test. Toxicol Res. 2011; 27(3): 133-5. https://doi.org/10.5487/tr.2011.27.3.133 https://doi.org/10.5487/TR.2011.27.3.133
  30. Kang SY, Park YC, Park YK. Thirteen-weel Repeated-dose Oral Toxicity Study of KOB03, A Polyherbal Medicine For Allergic Rhinitis, In Rats. Korean Jorunal Herbology. 2013; 28(1): 15-21. https://doi.org/10.6116/kjh.2013.28.1.15
  31. Kacaniova M, Durechova D, Vukovic N, Kantor A, Petrova J, Hleba L et at. Antimicrobial Activity of Drosera Rotundifolia L. Animal Science and Biotechnologies. 2017; 47(2): 366-9.
  32. Trevan JW. The Error of Determination of Toxicity. London: Production Royal Society. 1927; 101B: 483-514. https://doi.org/10.1098/rspb.1927.0030
  33. Huh IH. Toxicology. Seoul:Shin-il. 1993: 37.
  34. Kwon KR, Chu CS, Park HS, Kim MK, Cha BC, Lee E. Experimental Studies of Quantitative Evaluation Using HPLC and Safety of Sweet Bee Venom. Journal of Pharmacopuncture. 2007; 10(2): 81-6. https://doi.org/10.3831/kpi.2006.9.1.155 https://doi.org/10.3831/KPI.2007.10.2.081
  35. Kim SK, Kim KC, Lee BM. Mathematical and Statistical Characterization of LD_(50) Estimation. Toxicological Research. 2014; 20(4): 321-4.
  36. Seoul National University Physiology. Phsiology 7th. Seoul: Medical Moonhwasa. 2006: 269.
  37. Kim EJ, Kan DG, Lee AS, Choi DH, Cho KH, Kim SY, et al. Study on Kidney Tocixity of BDR-29 for Treatment Vascular Diseases in Rat. The Korean Journal of Oriental medical prescription. 2006; 16(2): 163-9.